Effect and significance of Vav3 to angiogenesis of gastric cancer cell line BGC823

檀碧波; 李勇

doi:10.3969/j.issn.1007-3969.2014.12.004

您当前的位置：

首页 >

文章列表页 >

Effect and significance of Vav3 to angiogenesis of gastric cancer cell line BGC823

更新时间：2025-12-31

- Effect and significance of Vav3 to angiogenesis of gastric cancer cell line BGC823
- China Oncology Vol. 24, Issue 12, Pages: 901-905(2014)
- 作者机构：
  
  河北医科大学第四医院外三科,河北,石家庄,050011
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2014.12.004
  CLC：
- Published Online：07 May 2015，
  
  Published：07 May 2014
- 稿件说明：
移动端阅览
檀碧波,李勇,范立侨等. Vav3对人胃癌细胞株BGC823血管生成基因的调节作用及意义[J]. 中国癌症杂志, 2014, 24(12): 901-905.

檀碧波, 李勇. Effect and significance of Vav3 to angiogenesis of gastric cancer cell line BGC823[J]. China Oncology, 2014, 24(12): 901-905.
檀碧波,李勇,范立侨等. Vav3对人胃癌细胞株BGC823血管生成基因的调节作用及意义[J]. 中国癌症杂志, 2014, 24(12): 901-905. DOI： 10.3969/j.issn.1007-3969.2014.12.004.

檀碧波, 李勇. Effect and significance of Vav3 to angiogenesis of gastric cancer cell line BGC823[J]. China Oncology, 2014, 24(12): 901-905. DOI： 10.3969/j.issn.1007-3969.2014.12.004.

摘要

背景与目的：研究发现Vav3基因在多种恶性肿瘤中表达异常，但Vav3与胃癌血管生成的关系尚不明确。本研究通过抑制内源性Vav3，观察其对胃癌细胞血管生成基因表达的影响，并探讨其机制和意义。方法：荧光定量RT-PCR及蛋白质印迹法(Western blot)检测人胃癌细胞株BGC823、胃上皮细胞株GES-1中Vav3的表达；合成针对Vav3的小干扰RNA(small interference RNA，siRNA)，并转染胃癌细胞株BGC823；MTT法检测转染前后胃癌细胞的活性；检测转染前后BGC823血管生成相关基因血管内皮生长因子-A(vascular endothelial growth factor-A，VEGF-A)、VEGF-C、VEGF-D、血管生成素-2(angiogenin-2，Ang-2)、血管生成抑制蛋白-1(vasohibin-1)表达的变化。结果：Vav3在胃癌BGC823细胞中的表达明显高于胃上皮细胞株GES-1(P0.01)；Vav3-siRNA转染BGC823细胞后，Vav3表达明显受到抑制(P均0.01)；MTT结果显示Vav3-siRNA转染后，BGC823活性明显降低(P0.05)；转染后B G C 8 2 3 中VEGF-C和Ang-2表达均较转染前降低(P均0 . 0 5 ) ，而vasohibin-1表达则升高(P均0.05)。结论：Vav3对部分胃癌细胞血管生成相关基因有调控作用，并可能通过这种作用促进肿瘤血管生成。

Abstract

Background and purpose: It was reported that Vav3 gene was expressed aberrantly in many cancers. But the relationship between Vav3 and angiogenesis genes of gastric cancer is still not clear. The purpose of this research was to investigate the effect and significance of Vav3 to angiogenesis of human gastric cancer cell line BGC823. Methods: The expressions of Vav3 in human gastric cancer cell line BGC823

gastric epithelial cell line GES-1 were tested by fluorecence quantitative RT-PCR and Western blot. Then Vav3-siRNA was synthesized and transfected into BGC823. Activity of BGC823 was measured with fluorecence quantitative RT-PCR and MTT assay. The expressions of angiogenesis related genes VEGF-A

VEGF-C

VEGF-D

angiogenin-1 (Ang-2)

vasohibin-1 were determined by qPCR and Western blot. Results: Expressions of Vav3 were detected higher in gastric cancer BGC823 cells than in GES-1 (P0.01). Expression of Vav3 was inhibited by Vav3-siRNA (P0.05). Activity of BGC823 was obviously inhibited after Vav3-siRNA transfected with MTT assay (P0.05). The expressions of VEGF-C

Ang-2 were lower in cells after transfected by Vav3-siRNA(both P0.05); expression of Vasohibin-1 increased after Vav3-siRNA transfected into BGC823 (P0.05). Conclusion: Vav3 gene can regulate some angiogenesis related genes

and Vav3 may promote angiogenesis of gastric cancer cells with this fuction.

关键词

Keywords

references

Views

1263

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Dihydromyricetin inhibits gastric cancer angiogenesis in vitro and in vivo through ERK/VEGFA/VEGFR2 signaling pathway

A study on the immunomicroenvironment characteristics of gastric cancer with neuroendocrine neoplasm and pure gastric cancer

Prospective trial on impact of oral nutritional supplements on elderly patients with nutritional risk after radical surgery for gastric cancer

Research progress and future perspectives of anticancer drug-induced interstitial lung disease/pneumonia in gastric cancer

Discovery and preliminary validation of five early gastric cancer biomarkers, including TAGLN2 and CTSD, based on serum proteomics

Related Author

温培楠

林明恕

金　冕

徐贤绸

陈龙云

ZHANG Yi

SUN Haidong

QU Xinglong

Related Institution

浙江省平阳人民医院普外科

湖北中医药大学基础医学院

Seventh People’s Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Surgical Oncology, Minhang Branch, Fudan University Shanghai Cancer Center

Department of Gastrointestinal Surgery, General Surgery, Zhongshan Hospital, Fudan University

AI问答

⁰