S100 calcium binding protein A4 expression and significance in gastric cancer

张天彪; 宿文辉; 赵滢

doi:10.3969/j.issn.1007-3969.2015.06.004

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S100 calcium binding protein A4 expression and significance in gastric cancer

更新时间：2025-12-31

- S100 calcium binding protein A4 expression and significance in gastric cancer
- China Oncology Vol. 25, Issue 6, Pages: 424-432(2015)
- 作者机构：
  
  1. 中国医科大学基础医学院生物化学与分子生物学教研室,辽宁,沈阳,110122
  2. 中国医科大学附属盛京医院胃肠营养外科,辽宁,沈阳,110004
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2015.06.004
  CLC：
- Published Online：14 August 2015，
  
  Published：14 August 2015
- 稿件说明：
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张天彪,宿文辉,赵滢,等. S100钙结合蛋白A4在胃癌组织中的表达及意义[J]. 中国癌症杂志, 2015, 25(6): 424-432.

张天彪, 宿文辉, 赵滢. S100 calcium binding protein A4 expression and significance in gastric cancer[J]. China Oncology, 2015, 25(6): 424-432.
张天彪,宿文辉,赵滢,等. S100钙结合蛋白A4在胃癌组织中的表达及意义[J]. 中国癌症杂志, 2015, 25(6): 424-432. DOI： 10.3969/j.issn.1007-3969.2015.06.004.

张天彪, 宿文辉, 赵滢. S100 calcium binding protein A4 expression and significance in gastric cancer[J]. China Oncology, 2015, 25(6): 424-432. DOI： 10.3969/j.issn.1007-3969.2015.06.004.

摘要

背景与目的：本研究探讨S100钙结合蛋白A4(S100 calcium-binding protein A4，S100A4)在胃癌旁正常组织、慢性胃炎、肠上皮化生、腺瘤样异型增生和胃癌的组织标本中的表达及与胃癌临床病理特征及预后的关系。方法：利用HE染色对所取胃组织标本进行病理组织学诊断；采用免疫组织化学方法检测标本S100A4蛋白的表达；实时定量聚合酶链反应(quantitative real-time pdymerase chain reaction，qRT-PCR)法检测S100A4mRNA表达；蛋白[质]印迹法(Western blot)检测S100A4蛋白的表达；采用Kaplan-Meier分析累积生存率。结果：S100A4蛋白和mRNA的表达按以下顺序逐渐增加：胃癌旁正常组织慢性胃炎肠上皮化生腺瘤样异型增生胃癌。S100A4表达水平与肿瘤大小、浸润深度、淋巴管浸润、淋巴结转移和肿瘤TNM分期有关，但与患者的年龄和性别无关。在较大直径、较深浸润、淋巴结转移的胃癌和肠型癌中可发现S100A4基因的过表达。采用Kaplan-Meier单因素分析显示，弱或中度S100A4基因表达的患者与不表达的患者相比有较低的累积生存率。结论：在胃癌旁正常组织-慢性胃炎-肠上皮化生-腺瘤样异型增生-胃癌过程中，S100A4 mRNA和蛋白表达逐渐增高；S100A4表达与胃癌肿瘤大小、侵袭深度、淋巴结转移和不良预后呈正相关；S100A4过表达参与胃癌发生和演进过程，可以作为反映胃癌恶性程度的生物学指标。

Abstract

Background and purpose: This study investigated the relationship between (S100 calcium-binding protein A4

S100A4) in chronic gastritis

intestinal metaplasia

dysplasia adenomatous

normal tissue tissue samples and expression in gastric cancer and clinical characteristics. Methods: HE staining of the use of gastric specimens taken for histopathological diagnosis; using immunohistochemistry to detect the expression of tissue S100A4 protein; qRT-PCR was used to detect mRNA expression of S100A4 gene; Western Blot detection of S100A4 gene encoding protein. Kaplan- Meier survival curves were used to distinguish and compare survival. Results: S100A4 protein and mRNA expression gradually increased in the following order: normal tissuegastritisintestinal metaplasiadysplasiacarcinoma. S100A4 levels with tumor size

depth of invasion

lymphatic invasion

lymph node metastasis and lymph node metastasis (TNM) staging

but with cancer patients independent of age and gender. Intestinal type (IT) cancer showed higher than the diffuse type (DT) of cancer S100A4 expression. S100A4 gene overexpression in a larger diameter

deeper invasion

lymph node metastasis of gastric and IT cancer was found. Kaplan-Meier method using univariate analysis showed weak expression of S100A4 gene is not expressed in patients with moderate or S100A4 gene have lower survival rates compared. Conclusion: Upregulated S100A4 gene may play an important role in the malignant transformation of epithelial cells in the stomach

and gastric cancer growth

invasion

metastasis and prognosis were positively correlated. Thus

S100A4 may be considered as a potential marker to show violations of gastric cancer. S100A4 expression in diffuse unique cancer can be used to distinguish between cancer and diffuse intestinal cancer

and can be used as a molecular characteristic distinguish two types of cancer.

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