The expressions of CDC4 and c-Myc in gastric cancer and their clinical signifieance

黄国全; 黎　晖; 张才全

doi:10.3969/j.issn.1007-3969.2015.12.003

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The expressions of CDC4 and c-Myc in gastric cancer and their clinical signifieance

更新时间：2025-12-31

- The expressions of CDC4 and c-Myc in gastric cancer and their clinical signifieance
- China Oncology Vol. 25, Issue 12, Pages: 933-939(2015)
- 作者机构：
  
  1. 湖北省恩施土家族苗族自治州中心医院胃肠外科,湖北,恩施,445000
  2. 重庆医科大学附属第一医院胃肠外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2015.12.003
  CLC：
- Published Online：03 February 2016，
  
  Published：03 February 2015
- 稿件说明：
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黄国全,黎　晖,张才全,等. CDC4及c-Myc在胃癌中的表达及临床意义[J]. 中国癌症杂志, 2015, 25(12): 933-939.

黄国全, 黎　晖, 张才全. The expressions of CDC4 and c-Myc in gastric cancer and their clinical signifieance[J]. China Oncology, 2015, 25(12): 933-939.
黄国全,黎　晖,张才全,等. CDC4及c-Myc在胃癌中的表达及临床意义[J]. 中国癌症杂志, 2015, 25(12): 933-939. DOI： 10.3969/j.issn.1007-3969.2015.12.003.

黄国全, 黎　晖, 张才全. The expressions of CDC4 and c-Myc in gastric cancer and their clinical signifieance[J]. China Oncology, 2015, 25(12): 933-939. DOI： 10.3969/j.issn.1007-3969.2015.12.003.

摘要

背景与目的：胃癌是全世界多发恶性肿瘤之一，其死亡率及术后复发率高。目前治疗胃癌的手段主要是手术及放化疗，但总体效果欠佳。随着经济及分子生物学的飞速发展，胃癌的早期诊断及分子靶向治疗成为全世界研究的热点。胃癌的发生、发展与抑癌基因的失活和癌基因的过表达相关。CDC4/Fbxw7为重要的抑癌基因。本实验通过研究CDC4(F框/WD-40域蛋白7，也称为FBXW7、Sel-10、Ago)及c-Myc蛋白在人胃癌组织中的表达，分析两者与胃癌临床病理特征的关系。方法：应用半定量逆转录聚合酶链反应(semiquantitative reverse transcription polymerase chain reaction，sRT-PCR)、免疫组化及蛋白［质］印迹法(Western blot)检测40例胃癌组织、癌旁组织及正常胃黏膜组织中CDC4、c-Myc的mRNA及蛋白的表达，并分析两者之间的相关性及与患者病理特征之间的关系。结果：胃癌中CDC4蛋白的表达明显低于癌旁组织及正常组织(P0.05)。而c-Myc蛋白的表达在胃癌中显著增高，阳性率同癌旁组织及正常组织比较，差异有统计学意义(P0.05)。CDC4、c-Myc蛋白及其mRNA的表达与胃癌患者的性别、年龄、肿瘤部位无关，而与肿瘤浸润深度、分化程度、TNM分期、有无淋巴结转移及有无远处转移有关(P0.05)。CDC4和c-Myc在mRNA水平和蛋白水平的表达均呈负相关(P0.05)。结论：CDC4在胃癌中表达量显著下降，可能与胃癌的浸润、分化及转移相关，其表达缺失可能导致c-Myc的过度表达。CDC4可能成为早期诊断、判断胃癌预后及浸润转移的生物学指标。

Abstract

Background and purpose: The gastric cancer is the highest incidence of malignant tumors in the world. The main treatment methods for gastric cancer are operation and chemotherapy. But the effect is not good. With the rapid development of economy and molecular biology

early diagnosis and molecular targeted therapy for gastric cancer has become a research hotspot. The oncogene overexpression and the anti-oncogene lower expression are closely related with gastric cancer. CDC4/FBXW7 is an anti-oncogene

but c-Myc is an oncogene. The previous research showed that CDC4 affected the expression of many oncogenes

such as Cyclin E. This study aimed to investigate the expression of CDC4 and c-Myc in gastric cancer and to elucidate the potential relationship between their expressions and clinical pathological characteristics. Methods: Semi-quantitative reverse transcription polymerase chain reaction (sRT-PCR)

immunohistochemistry and Western blot method were used to determine the mRNA and protein expressions of CDC4 and c-Myc in 40 specimens of gastric carcinoma tissues

corresponding adjacent tissues and normal mucosal tissues. The expressions of CDC4 and c-Myc and the clinical pathological characteristics were analyzed. Results: The protein expressions of CDC4 in gastric cancer tissues were significantly lower than those in adjacent tissues and normal mucosal tissues (P0.05)

whereas the protein expression of c-Myc in gastric cancer tissues was significantly higher than that in adjacent tissues and normal mucosal tissues (P0.05). The protein and mRNA expression of CDC4 and c-Myc were correlated with differentiation

TNM stage

lymph node metastasis

infiltration

but not with patients’ gender

age and site of cancer (P0.05). There was a significant negative correlation between CDC4 and c-Myc at the mRNA and protein expression levels (P0.05). Conclusion: The lower expression of CDC4 is correlated with differentiation

TNM stage

lymph node metastasis and infiltration. c-Myc overexpression is likely to be the CDC4 loss. It suggests that the loss of CDC4 may be a valuable marker for assessing the diagnosis and treatment and the prognosis of gastric cancer.

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