Let-7a/g/i targeted to Aurora-B in human osteosarcoma cells

周云飞; 刘家明; 陈宣银

doi:10.3969/j.issn.1007-3969.2015.12.008

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Let-7a/g/i targeted to Aurora-B in human osteosarcoma cells

更新时间：2025-12-31

- Let-7a/g/i targeted to Aurora-B in human osteosarcoma cells
- China Oncology Vol. 25, Issue 12, Pages: 966-971(2015)
- 作者机构：
  
  南昌大学第一附属医院骨科,江西,南昌,330006
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2015.12.008
  CLC：
- Published Online：03 February 2016，
  
  Published：03 February 2015
- 稿件说明：
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周云飞,刘家明,陈宣银,等. Let-7a/g/i在骨肉瘤细胞中靶向调控Aurora-B表达[J]. 中国癌症杂志, 2015, 25(12): 966-971.

周云飞, 刘家明, 陈宣银. Let-7a/g/i targeted to Aurora-B in human osteosarcoma cells[J]. China Oncology, 2015, 25(12): 966-971.
周云飞,刘家明,陈宣银,等. Let-7a/g/i在骨肉瘤细胞中靶向调控Aurora-B表达[J]. 中国癌症杂志, 2015, 25(12): 966-971. DOI： 10.3969/j.issn.1007-3969.2015.12.008.

周云飞, 刘家明, 陈宣银. Let-7a/g/i targeted to Aurora-B in human osteosarcoma cells[J]. China Oncology, 2015, 25(12): 966-971. DOI： 10.3969/j.issn.1007-3969.2015.12.008.

摘要

背景与目的：微小RNA(microRNA，miRNA)是一类具有重要调控作用的非编码小分子RNA，在多种肿瘤的发生、发展中起重要作用。该研究探讨可能在骨肉瘤细胞中调控Aurora-B激酶表达的miRNA，并为进一步探讨Aurora-B在骨肉瘤细胞恶性表型中的作用及调控机制奠定基础。方法：采用生物信息学预测软件(http://www.targetscan.org)以及荧光海肾素实验探讨可能靶向调控Aurora-B的miRNA；实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction，RTFQ-PCR)和蛋白[质]印迹法(Western blot)进一步验证骨肉瘤细胞系U2-OS和HOS细胞中调控Aurora-B表达的miRNA。结果：生物信息学预测和荧光海肾素实验显示，Aurora-B基因可能是let-7a/b/c/d/e/f/g/i的靶基因；RTFQ-PCR和Western blot检测结果显示，上调let-7a/g/i的U2-OS和HOS细胞中Aurora-B mRNA和蛋白的表达水平显著低于阴性对照组(阴性质粒转染细胞)；但是上调let-7b/c/d/e/f的U2-OS和HOS细胞中Aurora-B mRNA和蛋白的表达水平和阴性质粒转染细胞比较差异无统计学意义。结论：Let-7a/g/i可能负向调控Aurora-B在骨肉瘤细胞中的表达。

Abstract

Background and purpose: MicroRNA(miRNA) is a class of small non-coding RNA playing an important regulatory role in many tumors. This study investigated which miRNA might negatively regulate the expression of Aurora-B in osteosarcoma cells

and to lay the foundation for the further investigation of the effort and regulation of Aurora-B in osteosarcoma malignant phenotype. Methods: Bioinformatics prediction software (http://www.targetscan.org) and luciferase assays were used to investigate which miRNA might target to modulate the Aurora-B. Real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot assay were used to further verify which miRNA could negative regulate the expression of Aurora-B gene. Results: Bioinformatics prediction showed let-7 family have the possibility to modulate the expression of Aurora-B; Luciferase assays showed that Aurora-B might be the target gene of let-7a/b/c/d/e/f/g/i; RTFQ-PCR and Western blot analysis testified that both the expression levels of Aurora-B mRNA and Aurora-B protein were significantly decreased in Let-7a/g/i up-regulated U2-OS and HOS cells

compared to the cells in the negative control group; but in Let-7b/c/d/e/f up-regulated U2-OS and HOS cells

the expression levels of Aurora-B mRNA and Aurora-B protein have no significant difference

compared to the cells in the negative control group. Conclusion: Let-7a/g/i may downregulate the expression of Aurora-B in human osteosarcoma cells.

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