Expression of lncRNA PCGEM1 and AR co-localization in prostate cancer and tis significance

朱竹先; 余　晨; 邱忠民

doi:10.3969/j.issn.1007-3969.2016.04.006

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Expression of lncRNA PCGEM1 and AR co-localization in prostate cancer and tis significance

更新时间：2025-12-31

- Expression of lncRNA PCGEM1 and AR co-localization in prostate cancer and tis significance
- China Oncology Vol. 26, Issue 4, Pages: 320-325(2016)
- 作者机构：
  
  1. 同济大学附属同济医院肾内科,上海,200065
  2. 同济大学附属同济医院呼吸内科,上海,200065
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1007-3969.2016.04.006
  CLC：
- Published Online：16 June 2016，
  
  Published：16 June 2016
- 稿件说明：
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朱竹先,余　晨,邱忠民,等. LncRNA PCGEM1和雄激素受体在前列腺癌中的共定位表达及临床意义[J]. 中国癌症杂志, 2016, 26(4): 320-325.

朱竹先, 余　晨, 邱忠民. Expression of lncRNA PCGEM1 and AR co-localization in prostate cancer and tis significance[J]. China Oncology, 2016, 26(4): 320-325.
朱竹先,余　晨,邱忠民,等. LncRNA PCGEM1和雄激素受体在前列腺癌中的共定位表达及临床意义[J]. 中国癌症杂志, 2016, 26(4): 320-325. DOI： 10.3969/j.issn.1007-3969.2016.04.006.

朱竹先, 余　晨, 邱忠民. Expression of lncRNA PCGEM1 and AR co-localization in prostate cancer and tis significance[J]. China Oncology, 2016, 26(4): 320-325. DOI： 10.3969/j.issn.1007-3969.2016.04.006.

摘要

背景与目的：长链非编码RNA(long non-coding RNA，lncRNA)可参与肿瘤调控，有研究提示lncRNA PCGEM1可能影响雄激素受体(androgen receptor，AR)通路。本研究拟检测前列腺癌lncRNA PCGEM1和AR的表达情况，探讨其在前列腺癌中的表达及意义。方法：构建RNA核酸探针，应用荧光原位杂交(fluorescencein situ hybridization，FISH)技术检测前列腺癌lncRNA PCGEM1的表达情况，并使用荧光免疫组织化学技术检测前列腺癌AR的表达；采用RNA-pull down技术检测PCGEM1和AR的共同作用。结果：依赖AR的LNCaP细胞的PCGEM1表达水平显著高于非AR依赖性的PC3和DU145细胞，差异有统计学意义(P0.01)。前列腺癌组织中PCGEM1的表达与AR表达程度显著相关。与良性前列腺肿瘤相比，转移性肿瘤中的PCGEM1细胞核内定位显著不同，雄激素去势显著影响PCGEM1的表达及细胞内定位。而共定位检测发现，PCGEM1与AR之间存在一定程度的共定位现象。结论：本研究通过FISH研究发现，PCGEM1表达在雄激素去势条件下显著上调，而在转移性前列腺癌中，PCGEM1和AR的共定位表达表明，lncRNA PCGEM1和AR相互作用可能共同参与调控前列腺癌的恶性进展及转移。

Abstract

Background and purpose: Long non-coding RNA (lncRNA) could be an important player incancer biology. Recent studies showed that lncRNA PCGEM1 might be important in the regulation of androgen receptor (AR) signaling pathway. We tried to observe the expressions of lncRNA PCGEM1 and AR in prostate cancer

and investigate their role and significance in prostate cancer genesis and progress. Methods: The expression of lncRNA PCGEM1 was observed in prostate cancer by fluorescence in situ hybridization (FISH) technique. Then detection of AR was performed by immunofluorescence histochemistry methods. Their co-effective role was checked by RNA pull-down technique. Results: Compared with the AR-independent cell line such as PC3 or DU145

AR-dependent cell line such as LNCaP showed much higher expression of lncRNA PCGEM1 (P0.01). PCGEM1 and AR could be co-localized in most of these prostate cancer samples

especially in the metastasis samples. Moreover

androgen deprivation promoted the translocation of PCGEM1 into nucleus. RNA pull-down results also proved the co-effective role of PCGEM1 and AR. Conclusion: This study showed that lncRNA PCGEM1 was highly expressed in metastatic prostate cancer. It was related to the progress and malignant behavior of the prostate cancer. Its co-localization with AR may play an important role in prostate cancer genesis and progress.

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