肠炎和肠炎相关结直肠癌miRNA表达检测及生物信息学分析

殷　媛; 王　成; 戴　欣

doi:10.19401/j.cnki.1007-3639.2016.11.006

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肠炎和肠炎相关结直肠癌miRNA表达检测及生物信息学分析

更新时间：2025-12-31

- 肠炎和肠炎相关结直肠癌miRNA表达检测及生物信息学分析
- Investigation of key miRNAs and their target genes in inflammatory bowel diseases and colitisassociated colorectal cancers using miRNA profiling and bioinformatic tools
- 中国癌症杂志 2016年26卷第11期页码：916-921
- 作者机构：
  
  1. 江南大学附属医院肿瘤研究所,江苏,无锡,214062
  2. 南京军区南京总医院检验科,江苏,南京,210002
  3. 美世大学药学院，制药科学系，癌症纳米医学实验室，佐治亚州,亚特兰大,30341
- 作者简介：
- 基金信息：
  
  国家自然科学基金(81301784，81272299，81000867)。
- DOI：10.19401/j.cnki.1007-3639.2016.11.006
  中图分类号：
- 网络出版：2017-01-22，
  
  纸质出版：2016-01-22
- 稿件说明：
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殷　媛,王　成,戴　欣,等. 肠炎和肠炎相关结直肠癌miRNA表达检测及生物信息学分析[J]. 中国癌症杂志, 2016, 26(11): 916-921.

殷　媛, 王　成, 戴　欣. Investigation of key miRNAs and their target genes in inflammatory bowel diseases and colitisassociated colorectal cancers using miRNA profiling and bioinformatic tools[J]. China Oncology, 2016, 26(11): 916-921.
殷　媛,王　成,戴　欣,等. 肠炎和肠炎相关结直肠癌miRNA表达检测及生物信息学分析[J]. 中国癌症杂志, 2016, 26(11): 916-921. DOI： 10.19401/j.cnki.1007-3639.2016.11.006.

殷　媛, 王　成, 戴　欣. Investigation of key miRNAs and their target genes in inflammatory bowel diseases and colitisassociated colorectal cancers using miRNA profiling and bioinformatic tools[J]. China Oncology, 2016, 26(11): 916-921. DOI： 10.19401/j.cnki.1007-3639.2016.11.006.

摘要

背景与目的：炎症性肠病(inflammatory bowel diseases，IBD)为一组慢性肠道疾病，包括溃疡性结肠炎(ulcerative colitis，UC)与克罗恩病(Crohn’s disease，CD)。肠炎相关性结直肠癌(colitis-associated colorectal cancers，CAC)是由IBD癌化形成的一种恶性肿瘤。该研究通过检测UC、CD和CAC组织中相关微小核糖核酸(miRNA)的水平，初步探讨其作为肠炎癌转化分子标志物的可能性，并对在肠炎和CAC中显著变化的一组miRNAs进行靶基因归集和生物信息学分析，为以miRNAs为靶点的基因治疗提供理论和实验基础。方法：采用实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction，RTFQ-PCR)技术，检测13例UC组织、3例CD患者组织、12例CAC组织及8例正常肠组织中16种miRNAs的表达。通过生物信息学对显著变化的一组miRNA进行靶基因分析，将文献中已报导的所有靶基因进行汇总，并利用DAVID数据库对靶基因进行功能富集分析(GO-analysis)和信号转导通路富集分析(KEGG-analysis，BIOCARTA-analysis)。结果：炎症相关miR-146a和癌症相关miR-27a、miR-29a、miR-20a、miR-21在UC、CD和CAC中的表达都显著高于正常结肠组织，且这一组miRNA的靶基因都富集在癌症相关通路、免疫信号相关通路和炎癌转换相关通路上。结论：miR-146a、miR-27a、miR-29a、miR-20a和miR-21可能是参与肠炎向结直肠癌转化的一组miRNA。

Abstract

Background and purpose: Inflammatory bowel diseases (IBD) are a group of chronic intestinal diseases

including ulcerative colitis (UC) and Crohn’s disease (CD). This study identified differentially expressed miRNAs in UC

CD and colitis-associated colorectal cancers (CAC) to explore their potential as novel molecular biomarkers. Methods: Tissue samples were taken from 13 UC patients

3 CD patients

12 CAC patients

and 8 ageand gender-matched healthy controls. The miRNA expressions were detected by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) assay. Known targets of deregulated miRNAs were utilized using miRWalk 2.0 database

and subsequent bioinformatics analysis of these target genes was performed by DAVID software (GO-analysis

KEGG-analysis and BIOCARTA-analysis). Results: The data showed that miR-146a

miR-27a

miR-29a

miR-20a and miR-21 were upregulated in UC

CD and CAC tissues compared with normal control. Moreover

the target genes of these miRNAs were enriched in several key signal transduction pathways including cancer-related pathway and immunity-associated pathway. Conclusion: miR-146a

miR-27a

miR-29a

miR-20a and miR-21 may play important roles in the switching from IBD to CAC.

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