Background and purpose: XB130 protein plays an important role in proliferation and invasiveness of tumor cells. However

there is little research on

the role of XB130 protein in hepatocellular carcinoma (HCC) and the effect of XB130 is still unclear. This study investigated the role of XB130 gene in the proliferation of HCC cell and its potential mechanism. Methods: The protein expressions of XB130 in HCC cell lines

Huh7

HepG2 and SNU449

and liver cell line HL7702 were detected by Western blot. Huh7 cells were transfected with XB130-siRNA. Then cell viability was measured using cell counting kit-8 (CCK-8)

and cell cycle was examined by flow cytometry. Protein expressions of p-AKT

p-GSK3β

cyclin D1 and p-Rb were detected by Western blot

while mRNA expression levels of E2F/DP1 target genes (cyclin E1

c-Myc and PCNA) were measured by reverse transcription-polymerase chain reaction (RT-PCR). Results: The relative protein expressions of XB130 in Huh7

HepG2

SNU449 and HL7702 cells were 0.66±0.10

0.78±0.11

0.83±0.08 and 0.32±0.06

respectively. The difference between HCC cell lines and HL7702 cell line was statistically significant (P0.01). The transfection efficacy of XB130-siRNA was confirmed to be highly effective in Huh7 cells

and the viability of XB130-siRNA transfected Huh7 cells declined 72 h after transfection (P0.001). The ratio of Huh7 cells in G

0

/G

1

phase was increased

while the ratio in S or G

2

/M was decreased 48 h after XB130-siRNA transfection (P0.01). In addition

compared with negative control

protein expressions of p-AKT

p-GSK3β

cyclin D1 and p-Rb

and mRNA expression levels of cyclin E1

c-Myc and PCNA were all decreased in XB130-siRNA transfected Huh7 cells (P0.001). Conclusion: XB130 promotes the proliferation of HCC cells by regulating cell cycle-related proteins and downstream transcription factors.