With the increasing of incidence

differentiated thyroid cancer (DTC) has become a global concern.Nuclear medicine plays an important role in the diagnosis and treatment of DTC. With the development of new tracers

molecular imaging of nuclear medicine has shown great value in demonstrating the molecular characteristics and its expression level of DTC

and can be used to guide the precise target therapy based on molecular nuclear imaging in vivo. Here we present this review on the current status and future direction of the application of molecular nuclear imaging in evaluating the differentiated and non-differentiated phenotype characteristics in DTC.