miR-6775-3p在乳腺癌细胞中的表达及其对乳腺癌细胞生物学行为的影响

齐　正; 薛　云; 李宗良; 张小冲; 雷秋香; 张　提; 景立红; 刘登湘

doi:10.19401/j.cnki.1007-3639.2020.12.003

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miR-6775-3p在乳腺癌细胞中的表达及其对乳腺癌细胞生物学行为的影响

更新时间：2026-01-27

- miR-6775-3p在乳腺癌细胞中的表达及其对乳腺癌细胞生物学行为的影响
- Expression of miR-6775-3p in breast cancer cells and its effect on biological behavior of breast cancer cells
- 中国癌症杂志 2020年30卷第12期页码：984-990
- 作者机构：
  
  1. 邢台市人民医院检验科,河北,邢台,054031
  2. 邢台市第三医院检验科,河北,邢台,054031
  3. 邢台市人民医院核医学科,河北,邢台,054031
  4. 邢台市人民医院美容科,河北,邢台,054031
  5. 巨鹿县卫生学校内科,河北,邢台,055250
- 作者简介：
- 基金信息：
  
  河北省重点研发计划项目（182777122D）。
- DOI：10.19401/j.cnki.1007-3639.2020.12.003
  中图分类号：
- 网络出版：2021-01-08，
  
  纸质出版：2021-01-08
- 稿件说明：
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齐　正, 薛　云, 李宗良, 张小冲, 雷秋香, 张　提, 景立红, 刘登湘 . miR-6775-3p在乳腺癌细胞中的表达及其对乳腺癌细胞生物学行为的影响[J]. 中国癌症杂志, 2020, 30(12): 984-990.

QI Zheng, XUE Yun, LI Zongliang, et al. Expression of miR-6775-3p in breast cancer cells and its effect on biological behavior of breast cancer cells[J]. China Oncology, 2020, 30(12): 984-990.
齐　正, 薛　云, 李宗良, 张小冲, 雷秋香, 张　提, 景立红, 刘登湘 . miR-6775-3p在乳腺癌细胞中的表达及其对乳腺癌细胞生物学行为的影响[J]. 中国癌症杂志, 2020, 30(12): 984-990. DOI： 10.19401/j.cnki.1007-3639.2020.12.003.

QI Zheng, XUE Yun, LI Zongliang, et al. Expression of miR-6775-3p in breast cancer cells and its effect on biological behavior of breast cancer cells[J]. China Oncology, 2020, 30(12): 984-990. DOI： 10.19401/j.cnki.1007-3639.2020.12.003.

摘要

背景与目的：微小RNA（microRNA，miRNA）与肿瘤的发生、发展过程密切相关。探讨miR-6775-3p在乳腺癌细胞系中的表达及其对乳腺癌细胞生物学行为的影响。方法：通过实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction，RTFQ-PCR）检测4种乳腺癌细胞系MDA-MB-231、MDA-MB-453、MDA-MB-468和BT-549中miR-6775-3p的表达水平，选取miR-6775-3p表达水平最低的乳腺癌细胞系过表达miR-6775-3p后，采用细胞计数试剂盒（cell counting kit-8，CCK-8）法检测细胞的增殖情况，同时采用transwell迁移和侵袭实验分别检测细胞迁移和侵袭能力的变化。通过RTFQ-PCR和蛋白［质］印迹法（Western blot）检测miR-6775-3p过表达的乳腺癌细胞系中细胞周期蛋白依赖性蛋白激酶4（cyclin-dependent protein kinase 4，CDK4）和CDK6，以及侵袭转移标志物基质金属蛋白酶（matrix metalloproteinase，MMP）17和MMP24 mRNA以及蛋白的表达变化。结果：RTFQ-PCR结果显示，乳腺癌细胞系MDA-MB-453中miR-6775-3p的表达最低，在MDA-MB-453细胞中转染miR-6775-3p mimics后，miR-6775-3p的表达水平明显升高（P0.001）。CCK-8实验结果显示，MDA-MB-453细胞过表达miR-6775-3p后，细胞的增殖能力明显降低（P0.01）。Transwell迁移和侵袭实验结果显示，MDA-MB-453细胞过表达miR-6775-3p后，细胞的迁移（P0.001）和侵袭能力（P0.01）明显降低。RTFQ-PCR和Western blot实验结果显示，CDK4、CDK6及MMP17、MMP24的mRNA表达和蛋白水平均显著降低（P0.01）。结论：miR-6775-3p可能抑制乳腺癌细胞的增殖、迁移和侵袭能力。

Abstract

Background and purpose: MicroRNA (miRNA) is closely related to the occurrence and development of tumors. This study aimed to investigate the expression of miR-6775-3p in breast cancer cells and its effect on the biological characteristics of breast cancer cells. Methods: Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) was used to detect the expression level of miR-6775-3p in four breast cancer cell lines MDA-MB-231

MDA-MB-453

MDA-MB-468 and BT-549. After miR-6775-3p was over-expressed in breast cancer cells with the lowest expression level of miR-6775-3p

the proliferation of cells was detected by cell counting kit-8 (CCK-8) assay

and the migration and invasion ability of cells were detected by transwell migration and invasion assay. To further explore the molecular mechanism of miR-6775-3p in breast cancer

the expressions of cyclin-dependent protein kinase 4 (CDK4)

CDK6 and invasion and metastasis markers matrix metalloproteinase 17 (MMP17) and MMP24 in miR-6775-3p overexpressing breast cancer cells were detected by RTFQ-PCR and Western blot. Results: RTFQ-PCR results showed that the expression of miR-6775-3p was the lowest in breast cancer cell line MDA-MB-453

and the expression level of miR-6775-3p was significantly increased after transfection of miR-6775-3p mimics in MDA-MB-453 cells (P0.001). CCK-8 assay results showed that the cell proliferation activity was significantly decreased after miR-6775-3p was overexpressed in MDA-MB-453 cells (P0.01). Transwell migration and invasion assay results showed that the cell migration (P0.001) and invasion abilities (P0.01) were significantly decreased after miR-6775-3p was over-expressed in MDA-MB-453 cells. RTFQ-PCR and Western blot results showed that the expressions of cyclin-dependent protein kinases CDK4 and CDK6

as well as the mRNA and protein levels of cell invasion and metastasis markers MMP17 and MMP24 were significantly decreased (P0.01). Conclusion: miR-6775-3p may inhibit the proliferation

migration and invasion of breast cancer cells.

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