肿瘤类器官诊治平台的质量控制标准中国专家共识（2022年版）

中国抗癌协会肿瘤多学科诊疗专业委员会; 中国抗癌协会肿瘤内分泌专业委员会

doi:10.19401/j.cnki.1007-3639.2022.07.010

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肿瘤类器官诊治平台的质量控制标准中国专家共识（2022年版）

指南与共识 | 更新时间：2025-12-31

- 肿瘤类器官诊治平台的质量控制标准中国专家共识（2022年版）
- Chinese experts consensus on quality control standards for tumor organoids diagnosis and treatment platform (2022 version)
- 中国癌症杂志 2022年32卷第7期页码：657-668
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金(82073129);重庆市卫生适宜技术推广项目(2021jstg001)
- DOI：10.19401/j.cnki.1007-3639.2022.07.010
  中图分类号： R73-3
- 收稿：2022-06-10，
  
  纸质出版：2022-07-30
- 稿件说明：
移动端阅览
中国抗癌协会肿瘤多学科诊疗专业委员会, 中国抗癌协会肿瘤内分泌专业委员会. 肿瘤类器官诊治平台的质量控制标准中国专家共识（2022年版）[J]. 中国癌症杂志, 2022,32(7):657-668.

Society of Cancer Multidisciplinary Diagnosis and Treatment, China Anti-Cancer Association The, Society of Cancer Endocrinology, China Anti-Cancer Association The. Chinese experts consensus on quality control standards for tumor organoids diagnosis and treatment platform (2022 version)[J]. China Oncology, 2022, 32(7): 657-668.
中国抗癌协会肿瘤多学科诊疗专业委员会, 中国抗癌协会肿瘤内分泌专业委员会. 肿瘤类器官诊治平台的质量控制标准中国专家共识（2022年版）[J]. 中国癌症杂志, 2022,32(7):657-668. DOI： 10.19401/j.cnki.1007-3639.2022.07.010.

Society of Cancer Multidisciplinary Diagnosis and Treatment, China Anti-Cancer Association The, Society of Cancer Endocrinology, China Anti-Cancer Association The. Chinese experts consensus on quality control standards for tumor organoids diagnosis and treatment platform (2022 version)[J]. China Oncology, 2022, 32(7): 657-668. DOI： 10.19401/j.cnki.1007-3639.2022.07.010.

摘要

近年来

类器官技术的发展为开发新的、更接近真实病理生理学状态的人类癌症模型开辟了新途径。源自患者的肿瘤类器官保留了原始肿瘤的组织病理学特征、遗传特征和分子生物学特征等表型

不仅可作为识别和测试新型抗癌药物的优良模型

也可通过肿瘤类器官的药物敏感性检测反馈患者的临床反应

为肿瘤患者的个体化精准治疗提供可靠的依据。然而

与传统的组织样本库相比

活体类器官生物库的建立需要更严格的质量控制和更高水平的技术标准。随着类器官研究的不断深入

肿瘤类器官已广泛覆盖多种实体瘤

类器官技术亦在2017年被评为生命科学领域年度技术

但国内对肿瘤类器官构建、鉴定及药物敏感性检测尚无质量控制标准

同时亦缺乏肿瘤类器官用于精准治疗的专家共识。因此

根据全球范围内多个团队基于患者来源的肿瘤类器官的药物敏感性检测在肿瘤精准治疗中已发表的研究数据

中国抗癌协会肿瘤多学科诊疗专业委员会、中国抗癌协会肿瘤内分泌专业委员会针对肿瘤类器官药物敏感性检测的临床应用与质量控制制订了《肿瘤类器官诊治平台的质量控制标准中国专家共识（2022年版）》

希望通过本共识

提高中国临床工作者及医学研究者对于类器官药物敏感性检测的认识

规范类器官诊治平台的质量控制

指导类器官药物敏感性检测在国内肿瘤精准治疗方案选择中的临床应用。本共识将根据未来开展的基于类器官的各项研究成果定期更新。

Abstract

Recently

advances in organoid technology have opened new avenues for the development of novel models of human cancer. Patient-derived tumor organoids retain phenotypes such as histopathological

genetic and molecular features of the original tumor. The generation of cancer

more closer to the real pathophysiological status organoids from primary patient material enables a range of therapeutic agents to be tested in the resulting organoid cultures that not only can be an excellent and innovative platform for drug discovery

but also through sensitivity test of the drug candidates can predict patient response to therapy

thereby holding great promise for personalized medicine. However

compared to traditional biobanks

living biobanks from patient-derived organoids requires stricter quality control and higher level of technical standards. With further research of organoids

a variety of solid tumors have been covered. Organoid technology was named technology of the year in life sciences in 2017. There is no quality control standard for the construction

identification and drug-screening applications of patient-derived cancer organoids in China

and there is also a lack of experts consensus for drug sensitivity test to guide precise tumor treatment. Therefore

based on the advances of patient-derived tumor organoids at home and abroad in the precision medicine

the Multidisciplinary Diagnosis and Treatment Professional Committee of China Anti-cancer Association and the Tumor Endocrinology Professional Committee of China Anti-cancer Association have formulated “Chinese experts consensus on quality control standards for tumor organoids diagnosis and treatment platform (2022 version)”

which improves the understanding of Chinese clinicians and medical researchers on organoids’ drug sensitivity test

standardizes the quality of tumor organoids diagnosis and treatment platform

and guide its clinical application in the domestic precision medicine. This consensus will be regularly updated based on the further research on organoids.

关键词

Keywords

references

SUNG H , FERLAY J , SIEGEL R L , et al . Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J ] . CA Cancer J Clin , 2021 , 71 ( 3 ): 209 - 249 . DOI: 10.3322/caac.21660 http://doi.org/10.3322/caac.21660 https://onlinelibrary.wiley.com/doi/10.3322/caac.21660 https://onlinelibrary.wiley.com/doi/10.3322/caac.21660

BALANI S , NGUYEN L V , EAVES C J . Modeling the process of human tumorigenesis [J ] . Nat Commun , 2017 , 8 : 15422 . DOI: 10.1038/ncomms15422 http://doi.org/10.1038/ncomms15422 http://www.nature.com/articles/ncomms15422 http://www.nature.com/articles/ncomms15422

BEN-DAVID U , HA G , TSENG Y Y , et al . Patient-derived xenografts undergo mouse-specific tumor evolution [J ] . Nat Genet , 2017 , 49 ( 11 ): 1567 - 1575 . DOI: 10.1038/ng.3967 http://doi.org/10.1038/ng.3967 https://www.nature.com/articles/ng.3967 https://www.nature.com/articles/ng.3967

BYRNE A T , ALFEREZ D G , AMANT F , et al . Interrogating open issues in cancer precision medicine with patient-derived xenografts [J ] . Nat Rev Cancer , 2017 , 17 ( 4 ): 254 - 268 . DOI: 10.1038/nrc.2016.140 http://doi.org/10.1038/nrc.2016.140 https://doi.org/10.1038/nrc.2016.140 https://doi.org/10.1038/nrc.2016.140

DROST J , CLEVERS H . Organoids in cancer research [J ] . Nat Rev Cancer , 2018 , 18 ( 7 ): 407 - 418 . DOI: 10.1038/s41568-018-0007-6 http://doi.org/10.1038/s41568-018-0007-6 https://doi.org/10.1038/s41568-018-0007-6 https://doi.org/10.1038/s41568-018-0007-6

LI M , IZPISUA BELMONTE J C . Organoids-preclinical models of human disease [J ] . N Engl J Med , 2019 , 380 ( 6 ): 569 - 579 . DOI: 10.1056/NEJMra1806175 http://doi.org/10.1056/NEJMra1806175 http://www.nejm.org/doi/10.1056/NEJMra1806175 http://www.nejm.org/doi/10.1056/NEJMra1806175

PUSCHHOF J , PLEGUEZUELOS-MANZANO C , MARTINEZ-SILGADO A , et al . Intestinal organoid cocultures with microbes [J ] . Nat Protoc , 2021 , 16 ( 10 ): 4633 - 4649 . DOI: 10.1038/s41596-021-00589-z http://doi.org/10.1038/s41596-021-00589-z https://doi.org/10.1038/s41596-021-00589-z https://doi.org/10.1038/s41596-021-00589-z

SUGIMOTO S , KOBAYASHI E , FUJII M , et al . An organoid-based organ-repurposing approach to treat short bowel syndrome [J ] . Nature , 2021 , 592 ( 7852 ): 99 - 104 .

HENDRIKS D , ARTEGIANI B , HU H L , et al . Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver [J ] . Nat Protoc , 2021 , 16 ( 1 ): 182 - 217 . DOI: 10.1038/s41596-020-00411-2 http://doi.org/10.1038/s41596-020-00411-2 https://doi.org/10.1038/s41596-020-00411-2 https://doi.org/10.1038/s41596-020-00411-2

ROCK J R , ONAITIS M W , RAWLINS E L , et al . Basal cells as stem cells of the mouse trachea and human airway epithelium [J ] . Proc Natl Acad Sci USA , 2009 , 106 ( 31 ): 12771 - 12775 . DOI: 10.1073/pnas.0906850106 http://doi.org/10.1073/pnas.0906850106 https://pnas.org/doi/full/10.1073/pnas.0906850106 https://pnas.org/doi/full/10.1073/pnas.0906850106

VAN DE WETERING M , FRANCIES H E , FRANCIS J M , et al . Prospective derivation of a living organoid biobank of colorectal cancer patients [J ] . Cell , 2015 , 161 ( 4 ): 933 - 945 . DOI: 10.1016/j.cell.2015.03.053 http://doi.org/10.1016/j.cell.2015.03.053 https://linkinghub.elsevier.com/retrieve/pii/S0092867415003736 https://linkinghub.elsevier.com/retrieve/pii/S0092867415003736

FUJII M , SHIMOKAWA M , DATE S , et al . A colorectal tumor organoid library demonstrates progressive loss of niche factor requirements during tumorigenesis [J ] . Cell Stem Cell , 2016 , 18 ( 6 ): 827 - 838 . DOI: 10.1016/j.stem.2016.04.003 http://doi.org/10.1016/j.stem.2016.04.003 https://linkinghub.elsevier.com/retrieve/pii/S1934590916300467 https://linkinghub.elsevier.com/retrieve/pii/S1934590916300467

WEEBER F , VAN DE WETERING M , HOOGSTRAAT M , et al . Preserved genetic diversity in organoids cultured from biopsies of human colorectal cancer metastases [J ] . Proc Natl Acad Sci USA , 2015 , 112 ( 43 ): 13308 - 13311 . DOI: 10.1073/pnas.1516689112 http://doi.org/10.1073/pnas.1516689112 https://pnas.org/doi/full/10.1073/pnas.1516689112 https://pnas.org/doi/full/10.1073/pnas.1516689112

BOJ S F , HWANG C I , BAKER L A , et al . Organoid models of human and mouse ductal pancreatic cancer [J ] . Cell , 2015 , 160 ( 1/2 ): 324 - 338 . DOI: 10.1016/j.cell.2014.12.021 http://doi.org/10.1016/j.cell.2014.12.021 https://linkinghub.elsevier.com/retrieve/pii/S009286741401592X https://linkinghub.elsevier.com/retrieve/pii/S009286741401592X

BARTFELD S , BAYRAM T , VAN DE WETERING M , et al . In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection [J ] . Gastroenterology , 2015 , 148 ( 1 ): 126 - 136 .e6. DOI: 10.1053/j.gastro.2014.09.042 http://doi.org/10.1053/j.gastro.2014.09.042 https://linkinghub.elsevier.com/retrieve/pii/S0016508514012037 https://linkinghub.elsevier.com/retrieve/pii/S0016508514012037

BROUTIER L , MASTROGIOVANNI G , VERSTEGEN M M , et al . Human primary liver cancer-derived organoid cultures for disease modeling and drug screening [J ] . Nat Med , 2017 , 23 ( 12 ): 1424 - 1435 . DOI: 10.1038/nm.4438 http://doi.org/10.1038/nm.4438 https://www.nature.com/articles/nm.4438 https://www.nature.com/articles/nm.4438

SACHS N , DE LIGT J , KOPPER O , et al . A living biobank of breast cancer organoids captures disease heterogeneity [J ] . Cell , 2018 , 172 ( 1/2 ): 373 - 386 .e10. DOI: 10.1016/j.cell.2017.11.010 http://doi.org/10.1016/j.cell.2017.11.010 https://linkinghub.elsevier.com/retrieve/pii/S0092867417313193 https://linkinghub.elsevier.com/retrieve/pii/S0092867417313193

GAO D , VELA I , SBONER A , et al . Organoid cultures derived from patients with advanced prostate cancer [J ] . Cell , 2014 , 159 ( 1 ): 176 - 187 . DOI: 10.1016/j.cell.2014.08.016 http://doi.org/10.1016/j.cell.2014.08.016 https://linkinghub.elsevier.com/retrieve/pii/S0092867414010472 https://linkinghub.elsevier.com/retrieve/pii/S0092867414010472

DRIEHUIS E , KRETZSCHMAR K , CLEVERS H . Establishment of patient-derived cancer organoids for drug-screening applications [J ] . Nat Protoc , 2020 , 15 ( 10 ): 3380 - 3409 . DOI: 10.1038/s41596-020-0379-4 http://doi.org/10.1038/s41596-020-0379-4 https://doi.org/10.1038/s41596-020-0379-4 https://doi.org/10.1038/s41596-020-0379-4

OOFT S N , WEEBER F , DIJKSTRA K K , et al . Patient-derived organoids can predict response to chemotherapy in metastatic colorectal cancer patients [J ] . Sci Transl Med , 2019 , 11 ( 513 ): eaay2574.

VLACHOGIANNIS G , HEDAYAT S , VATSIOU A , et al . Patient-derived organoids model treatment response of metastatic gastrointestinal cancers [J ] . Science , 2018 , 359 ( 6378 ): 920 - 926 . DOI: 10.1126/science.aao2774 http://doi.org/10.1126/science.aao2774 https://www.science.org/doi/10.1126/science.aao2774 https://www.science.org/doi/10.1126/science.aao2774

SEPPÄLÄ T T , ZIMMERMAN J W , SURI R , et al . Precision medicine in pancreatic cancer: patient derived organoid pharmacotyping is a predictive biomarker of clinical treatment response [J ] . Clin Cancer Res , 2022 : 4165 .

CHEN P , ZHANG X , DING R B , et al . Patient-derived organoids can guide personalized-therapies for patients with advanced breast cancer [J ] . Adv Sci (Weinh) , 2021 , 8 ( 22 ): e2101176.

YAO Y , XU X Y , YANG L F , et al . Patient-derived organoids predict chemoradiation responses of locally advanced rectal cancer [J ] . Cell Stem Cell , 2020 , 26 ( 1 ): 17 - 26 .e6. DOI: 10.1016/j.stem.2019.10.010 http://doi.org/10.1016/j.stem.2019.10.010 https://linkinghub.elsevier.com/retrieve/pii/S193459091930431X https://linkinghub.elsevier.com/retrieve/pii/S193459091930431X

NOZAKI K , MOCHIZUKI W , MATSUMOTO Y , et al . Co-culture with intestinal epithelial organoids allows efficient expansion and motility analysis of intraepithelial lymphocytes [J ] . J Gastroenterol , 2016 , 51 ( 3 ): 206 - 213 . DOI: 10.1007/s00535-016-1170-8 http://doi.org/10.1007/s00535-016-1170-8 https://link.springer.com/10.1007/s00535-016-1170-8 https://link.springer.com/10.1007/s00535-016-1170-8

CATTANEO C M , DIJKSTRA K K , FANCHI L F , et al . Tumor organoid-T-cell coculture systems [J ] . Nat Protoc , 2020 , 15 ( 1 ): 15 - 39 . DOI: 10.1038/s41596-019-0232-9 http://doi.org/10.1038/s41596-019-0232-9 https://doi.org/10.1038/s41596-019-0232-9 https://doi.org/10.1038/s41596-019-0232-9

PAULI C , HOPKINS B D , PRANDI D , et al . Personalized in vitro and in vivo cancer models to guide precision medicine [J ] . Cancer Discov , 2017 , 7 ( 5 ): 462 - 477 . DOI: 10.1158/2159-8290.CD-16-1154 http://doi.org/10.1158/2159-8290.CD-16-1154 https://aacrjournals.org/cancerdiscovery/article/7/5/462/6239/Personalized-In-Vitro-and-In-Vivo-Cancer-Models-to https://aacrjournals.org/cancerdiscovery/article/7/5/462/6239/Personalized-In-Vitro-and-In-Vivo-Cancer-Models-to

KOPPER O , DE WITTE C J , LÕHMUSSAAR K , et al . An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity [J ] . Nat Med , 2019 , 25 ( 5 ): 838 - 849 . DOI: 10.1038/s41591-019-0422-6 http://doi.org/10.1038/s41591-019-0422-6 https://doi.org/10.1038/s41591-019-0422-6 https://doi.org/10.1038/s41591-019-0422-6

LINKOUS A , BALAMATSIAS D , SNUDERL M , et al . Modeling patient-derived glioblastoma with cerebral organoids [J ] . Cell Rep , 2019 , 26 ( 12 ): 3203 - 3211 .e5. DOI: 10.1016/j.celrep.2019.02.063 http://doi.org/10.1016/j.celrep.2019.02.063 https://linkinghub.elsevier.com/retrieve/pii/S2211124719302426 https://linkinghub.elsevier.com/retrieve/pii/S2211124719302426

UBINK I , BOLHAQUEIRO A C F , ELIAS S G , et al . Organoids from colorectal peritoneal metastases as a platform for improving hyperthermic intraperitoneal chemotherapy [J ] . Br J Surg , 2019 , 106 ( 10 ): 1404 - 1414 . DOI: 10.1002/bjs.11206 http://doi.org/10.1002/bjs.11206 https://academic.oup.com/bjs/article/106/10/1404-1414/6120874 https://academic.oup.com/bjs/article/106/10/1404-1414/6120874

CHEN H , GOTIMER K , DE SOUZA C , et al . Short-term organoid culture for drug sensitivity testing of high-grade serous carcinoma [J ] . Gynecol Oncol , 2020 , 157 ( 3 ): 783 - 792 . DOI: 10.1016/j.ygyno.2020.03.026 http://doi.org/10.1016/j.ygyno.2020.03.026 https://linkinghub.elsevier.com/retrieve/pii/S0090825820302511 https://linkinghub.elsevier.com/retrieve/pii/S0090825820302511

DRIEHUIS E , KOLDERS S , SPELIER S , et al . Oral mucosal organoids as a potential platform for personalized cancer therapy [J ] . Cancer Discov , 2019 , 9 ( 7 ): 852 - 871 . DOI: 10.1158/2159-8290.CD-18-1522 http://doi.org/10.1158/2159-8290.CD-18-1522 https://aacrjournals.org/cancerdiscovery/article/9/7/852/42021/Oral-Mucosal-Organoids-as-a-Potential-Platform-for https://aacrjournals.org/cancerdiscovery/article/9/7/852/42021/Oral-Mucosal-Organoids-as-a-Potential-Platform-for

LÕHMUSSAAR K , OKA R , ESPEJO VALLE-INCLAN J , et al . Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer [J ] . Cell Stem Cell , 2021 , 28 ( 8 ): 1380 - 1396 .e6. DOI: 10.1016/j.stem.2021.03.012 http://doi.org/10.1016/j.stem.2021.03.012 https://linkinghub.elsevier.com/retrieve/pii/S1934590921001181 https://linkinghub.elsevier.com/retrieve/pii/S1934590921001181

DROST J , KARTHAUS W R , GAO D , et al . Organoid culture systems for prostate epithelial and cancer tissue [J ] . Nat Protoc , 2016 , 11 ( 2 ): 347 - 358 . DOI: 10.1038/nprot.2016.006 http://doi.org/10.1038/nprot.2016.006 https://doi.org/10.1038/nprot.2016.006 https://doi.org/10.1038/nprot.2016.006

MARSEE A , ROOS F J M , VERSTEGEN M M A , et al . Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids [J ] . Cell Stem Cell , 2021 , 28 ( 5 ): 816 - 832 . DOI: 10.1016/j.stem.2021.04.005 http://doi.org/10.1016/j.stem.2021.04.005 https://linkinghub.elsevier.com/retrieve/pii/S1934590921001624 https://linkinghub.elsevier.com/retrieve/pii/S1934590921001624

VOTANOPOULOS K I , FORSYTHE S , SIVAKUMAR H , et al . Model of patient-specific immune-enhanced organoids for immunotherapy screening: feasibility study [J ] . Ann Surg Oncol , 2020 , 27 ( 6 ): 1956 - 1967 . DOI: 10.1245/s10434-019-08143-8 http://doi.org/10.1245/s10434-019-08143-8 https://doi.org/10.1245/s10434-019-08143-8 https://doi.org/10.1245/s10434-019-08143-8

OU L L , WANG H S , HUANG H , et al . Preclinical platforms to study therapeutic efficacy of human γδ T cells [J ] . Clin Transl Med , 2022 , 12 ( 6 ): e814.

MAGILL S T , VASUDEVAN H N , SEO K , et al . Multiplatform genomic profiling and magnetic resonance imaging identify mechanisms underlying intratumor heterogeneity in meningioma [J ] . Nat Commun , 2020 , 11 ( 1 ): 4803.

NYS C , LEE Y L , ROOSE H , et al . Exploring stem cell biology in pituitary tumors and derived organoids [J ] . Endocr Relat Cancer , 2022 , 29 ( 7 ): 427 - 450 . DOI: 10.1530/ERC-21-0374 http://doi.org/10.1530/ERC-21-0374 https://erc.bioscientifica.com/view/journals/erc/29/7/ERC-21-0374.xml https://erc.bioscientifica.com/view/journals/erc/29/7/ERC-21-0374.xml

WENSINK G E , ELIAS S G , MULLENDERS J , et al . Patient-derived organoids as a predictive biomarker for treatment response in cancer patients [J ] . NPJ Precis Oncol , 2021 , 5 ( 1 ): 30 .

BANDA M , MCKIM K L , MYERS M B , et al . Outgrowth of erlotinib-resistant subpopulations recapitulated in patient-derived lung tumor spheroids and organoids [J ] . PLoS One , 2020 , 15 ( 9 ): e0238862.

DE WITTE C J , ESPEJO VALLE-INCLAN J , HAMI N , et al . Patient-derived ovarian cancer organoids mimic clinical response and exhibit heterogeneous inter- and intrapatient drug responses [J ] . Cell Rep , 2020 , 31 ( 11 ): 107762.

LEE S H , HU W H , MATULAY J T , et al . Tumor evolution and drug response in patient-derived organoid models of bladder cancer [J ] . Cell , 2018 , 173 ( 2 ): 515 - 528 .e17. DOI: 10.1016/j.cell.2018.03.017 http://doi.org/10.1016/j.cell.2018.03.017 https://linkinghub.elsevier.com/retrieve/pii/S0092867418302976 https://linkinghub.elsevier.com/retrieve/pii/S0092867418302976

SHARICK J T , WALSH C M , SPRACKLING C M , et al . Metabolic heterogeneity in patient tumor-derived organoids by primary site and drug treatment [J ] . Front Oncol , 2020 , 10 : 553 . DOI: 10.3389/fonc.2020.00553 http://doi.org/10.3389/fonc.2020.00553 https://www.frontiersin.org/article/10.3389/fonc.2020.00553/full https://www.frontiersin.org/article/10.3389/fonc.2020.00553/full

UKAI S , HONMA R , SAKAMOTO N , et al . Molecular biological analysis of 5-FU-resistant gastric cancer organoids; KHDRBS3 contributes to the attainment of features of cancer stem cell [J ] . Oncogene , 2020 , 39 ( 50 ): 7265 - 7278 . DOI: 10.1038/s41388-020-01492-9 http://doi.org/10.1038/s41388-020-01492-9 https://doi.org/10.1038/s41388-020-01492-9 https://doi.org/10.1038/s41388-020-01492-9

DRAIN A P , ZAHIR N , NORTHEY J J , et al . Matrix compliance permits NF-κB activation to drive therapy resistance in breast cancer [J ] . J Exp Med , 2021 , 218 ( 5 ): e20191360.

CHEN B S , ALVARADO D M , ITICOVICI M , et al . Interferon-induced IDO1 mediates radiation resistance and is a therapeutic target in colorectal cancer [J ] . Cancer Immunol Res , 2020 , 8 ( 4 ): 451 - 464 . DOI: 10.1158/2326-6066.CIR-19-0282 http://doi.org/10.1158/2326-6066.CIR-19-0282 https://aacrjournals.org/cancerimmunolres/article/8/4/451/467174/Interferon-Induced-IDO1-Mediates-Radiation https://aacrjournals.org/cancerimmunolres/article/8/4/451/467174/Interferon-Induced-IDO1-Mediates-Radiation

PUCA L , BAREJA R , PRANDI D , et al . Patient derived organoids to model rare prostate cancer phenotypes [J ] . Nat Commun , 2018 , 9 ( 1 ): 2404.

BOLLINGER J , MAY E , MATHEWS D , et al . Patients’ perspectives on the derivation and use of organoids [J ] . Stem Cell Reports , 2021 , 16 ( 8 ): 1874 - 1883 . DOI: 10.1016/j.stemcr.2021.07.004 http://doi.org/10.1016/j.stemcr.2021.07.004 https://linkinghub.elsevier.com/retrieve/pii/S2213671121003295 https://linkinghub.elsevier.com/retrieve/pii/S2213671121003295

BREDENOORD A L , CLEVERS H , KNOBLICH J A . Human tissues in a dish: the research and ethical implications of organoid technology [J ] . Science , 2017 , 355 ( 6322 ): eaaf9414.

BOERS S N , VAN DELDEN J J M , BREDENOORD A L . Broad consent is consent for governance [J ] . Am J Bioeth , 2015 , 15 ( 9 ): 53 - 55 .

SCHUTGENS F , CLEVERS H . Human organoids: tools for understanding biology and treating diseases [J ] . Annu Rev Pathol , 2020 , 15 : 211 - 234 . DOI: 10.1146/annurev-pathmechdis-012419-032611 http://doi.org/10.1146/annurev-pathmechdis-012419-032611 https://www.annualreviews.org/doi/10.1146/annurev-pathmechdis-012419-032611 https://www.annualreviews.org/doi/10.1146/annurev-pathmechdis-012419-032611

BOERS S N , VAN DELDEN J J , CLEVERS H , et al . Organoid biobanking: identifying the ethics: organoids revive old and raise new ethical challenges for basic research and therapeutic use [J ] . EMBO Rep , 2016 , 17 ( 7 ): 938 - 941 . DOI: 10.15252/embr.201642613 http://doi.org/10.15252/embr.201642613 https://onlinelibrary.wiley.com/doi/10.15252/embr.201642613 https://onlinelibrary.wiley.com/doi/10.15252/embr.201642613

BALLARD D , BOYER C , ALEXANDER J . Organoids-preclinical models of human disease [J ] . N Engl J Med , 2019 , 380 ( 20 ): 1981 - 1982 . DOI: 10.1056/NEJMc1903253 http://doi.org/10.1056/NEJMc1903253 http://www.nejm.org/doi/10.1056/NEJMc1903253 http://www.nejm.org/doi/10.1056/NEJMc1903253

CHALABI M , FANCHI L F , DIJKSTRA K K , et al . Neoadjuvant immunotherapy leads to pathological responses in MMR-proficient and MMR-deficient early-stage colon cancers [J ] . Nat Med , 2020 , 26 ( 4 ): 566 - 576 . DOI: 10.1038/s41591-020-0805-8 http://doi.org/10.1038/s41591-020-0805-8 https://doi.org/10.1038/s41591-020-0805-8 https://doi.org/10.1038/s41591-020-0805-8

NARASIMHAN V , WRIGHT J A , CHURCHILL M , et al . Medium-throughput drug screening of patient-derived organoids from colorectal peritoneal metastases to direct personalized therapy [J ] . Clin Cancer Res , 2020 , 26 ( 14 ): 3662 - 3670 . DOI: 10.1158/1078-0432.CCR-20-0073 http://doi.org/10.1158/1078-0432.CCR-20-0073 https://aacrjournals.org/clincancerres/article/26/14/3662/9625/Medium-throughput-Drug-Screening-of-Patient https://aacrjournals.org/clincancerres/article/26/14/3662/9625/Medium-throughput-Drug-Screening-of-Patient

STEELE N G , CHAKRABARTI J , WANG J , et al . An organoid-based preclinical model of human gastric cancer [J ] . Cell Mol Gastroenterol Hepatol , 2019 , 7 ( 1 ): 161 - 184 . DOI: 10.1016/j.jcmgh.2018.09.008 http://doi.org/10.1016/j.jcmgh.2018.09.008 https://linkinghub.elsevier.com/retrieve/pii/S2352345X18301309 https://linkinghub.elsevier.com/retrieve/pii/S2352345X18301309

TIRIAC H , BELLEAU P , ENGLE D D , et al . Organoid profiling identifies common responders to chemotherapy in pancreatic cancer [J ] . Cancer Discov , 2018 , 8 ( 9 ): 1112 - 1129 . DOI: 10.1158/2159-8290.CD-18-0349 http://doi.org/10.1158/2159-8290.CD-18-0349 https://aacrjournals.org/cancerdiscovery/article/8/9/1112/10165/Organoid-Profiling-Identifies-Common-Responders-to https://aacrjournals.org/cancerdiscovery/article/8/9/1112/10165/Organoid-Profiling-Identifies-Common-Responders-to

LI X D , FRANCIES H E , SECRIER M , et al . Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics [J ] . Nat Commun , 2018 , 9 ( 1 ): 2983.

PHAN N , HONG J J , TOFIG B , et al . A simple high-throughput approach identifies actionable drug sensitivities in patient-derived tumor organoids [J ] . Commun Biol , 2019 , 2 : 78 . DOI: 10.1038/s42003-019-0305-x http://doi.org/10.1038/s42003-019-0305-x https://doi.org/10.1038/s42003-019-0305-x https://doi.org/10.1038/s42003-019-0305-x

MAZZOCCHI A R , RAJAN S A P , VOTANOPOULOS K I , et al . In vitro patient-derived 3D mesothelioma tumor organoids facilitate patient-centric therapeutic screening [J ] . Sci Rep , 2018 , 8 ( 1 ): 2886.

JACOB F , SALINAS R D , ZHANG D Y , et al . A patient-derived glioblastoma organoid model and biobank recapitulates inter- and intra-tumoral heterogeneity [J ] . Cell , 2020 , 180 ( 1 ): 188 - 204 .e22. DOI: 10.1016/j.cell.2019.11.036 http://doi.org/10.1016/j.cell.2019.11.036 https://linkinghub.elsevier.com/retrieve/pii/S0092867419313212 https://linkinghub.elsevier.com/retrieve/pii/S0092867419313212

WANG T , PAN W J , ZHENG H , et al . Accuracy of using a patient-derived tumor organoid culture model to predict the response to chemotherapy regimens in stage Ⅳ colorectal cancer: a blinded study [J ] . Dis Colon Rectum , 2021 , 64 ( 7 ): 833 - 850 . DOI: 10.1097/DCR.0000000000001971 http://doi.org/10.1097/DCR.0000000000001971 https://journals.lww.com/10.1097/DCR.0000000000001971 https://journals.lww.com/10.1097/DCR.0000000000001971

LANCASTER M A , RENNER M , MARTIN C A , et al . Cerebral organoids model human brain development and microcephaly [J ] . Nature , 2013 , 501 ( 7467 ): 373 - 379 . DOI: 10.1038/nature12517 http://doi.org/10.1038/nature12517 https://doi.org/10.1038/nature12517 https://doi.org/10.1038/nature12517

NISHINAKAMURA R . Human kidney organoids: progress and remaining challenges [J ] . Nat Rev Nephrol , 2019 , 15 ( 10 ): 613 - 624 . DOI: 10.1038/s41581-019-0176-x http://doi.org/10.1038/s41581-019-0176-x https://doi.org/10.1038/s41581-019-0176-x https://doi.org/10.1038/s41581-019-0176-x

LUKONIN I , SERRA D , CHALLET MEYLAN L , et al . Phenotypic landscape of intestinal organoid regeneration [J ] . Nature , 2020 , 586 ( 7828 ): 275 - 280 . DOI: 10.1038/s41586-020-2776-9 http://doi.org/10.1038/s41586-020-2776-9 https://doi.org/10.1038/s41586-020-2776-9 https://doi.org/10.1038/s41586-020-2776-9

GANESH K , WU C , O’ROURKE K P , et al . A rectal cancer organoid platform to study individual responses to chemoradiation [J ] . Nat Med , 2019 , 25 ( 10 ): 1607 - 1614 . DOI: 10.1038/s41591-019-0584-2 http://doi.org/10.1038/s41591-019-0584-2 https://doi.org/10.1038/s41591-019-0584-2 https://doi.org/10.1038/s41591-019-0584-2

SHI R S , RADULOVICH N , NG C , et al . Organoid cultures as preclinical models of non-small cell lung cancer [J ] . Clin Cancer Res , 2020 , 26 ( 5 ): 1162 - 1174 . DOI: 10.1158/1078-0432.CCR-19-1376 http://doi.org/10.1158/1078-0432.CCR-19-1376 https://aacrjournals.org/clincancerres/article/26/5/1162/83141/Organoid-Cultures-as-Preclinical-Models-of-Non https://aacrjournals.org/clincancerres/article/26/5/1162/83141/Organoid-Cultures-as-Preclinical-Models-of-Non

KIM M , MUN H , SUNG C O , et al . Patient-derived lung cancer organoids as in vitro cancer models for therapeutic screening [J ] . Nat Commun , 2019 , 10 ( 1 ): 3991.

ROSENBLUTH J M , SCHACKMANN R C J , GRAY G K , et al . Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages [J ] . Nat Commun , 2020 , 11 ( 1 ): 1711.

YAN H H N , SIU H C , LAW S , et al . A comprehensive human gastric cancer organoid biobank captures tumor subtype heterogeneity and enables therapeutic screening [J ] . Cell Stem Cell , 2018 , 23 ( 6 ): 882 - 897 .e11. DOI: 10.1016/j.stem.2018.09.016 http://doi.org/10.1016/j.stem.2018.09.016 https://linkinghub.elsevier.com/retrieve/pii/S1934590918304806 https://linkinghub.elsevier.com/retrieve/pii/S1934590918304806

YAN H H N , SIU H C , HO S L , et al . Organoid cultures of early-onset colorectal cancers reveal distinct and rare genetic profiles [J ] . Gut , 2020 , 69 ( 12 ): 2165 - 2179 . DOI: 10.1136/gutjnl-2019-320019 http://doi.org/10.1136/gutjnl-2019-320019 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2019-320019 https://gut.bmj.com/lookup/doi/10.1136/gutjnl-2019-320019

HU H L , GEHART H , ARTEGIANI B , et al . Long-term expansion of functional mouse and human hepatocytes as 3D organoids [J ] . Cell , 2018 , 175 ( 6 ): 1591 - 1606 .e19. DOI: 10.1016/j.cell.2018.11.013 http://doi.org/10.1016/j.cell.2018.11.013 https://linkinghub.elsevier.com/retrieve/pii/S0092867418315058 https://linkinghub.elsevier.com/retrieve/pii/S0092867418315058

SHIOTA J , SAMUELSON L C , RAZUMILAVA N . Hepatobiliary organoids and their applications for studies of liver health and disease: are we there yet? [J ] . Hepatology , 2021 , 74 ( 4 ): 2251 - 2263 . DOI: 10.1002/hep.31772 http://doi.org/10.1002/hep.31772 https://onlinelibrary.wiley.com/doi/10.1002/hep.31772 https://onlinelibrary.wiley.com/doi/10.1002/hep.31772

YUN J , LEE S H , KIM S Y , et al . Antitumor activity of amivantamab (JNJ-61186372), an EGFR-MET bispecific antibody, in diverse models of EGFR exon 20 insertion-driven NSCLC [J ] . Cancer Discov , 2020 , 10 ( 8 ): 1194 - 1209 . DOI: 10.1158/2159-8290.CD-20-0116 http://doi.org/10.1158/2159-8290.CD-20-0116 https://aacrjournals.org/cancerdiscovery/article/10/8/1194/2590/Antitumor-Activity-of-Amivantamab-JNJ-61186372-an https://aacrjournals.org/cancerdiscovery/article/10/8/1194/2590/Antitumor-Activity-of-Amivantamab-JNJ-61186372-an

WANG Y , JIANG T , QIN Z , et al . HER2 exon 20 insertions in non-small cell lung cancer are sensitive to the irreversible pan-HER receptor tyrosine kinase inhibitor pyrotinib [J ] . Ann Oncol , 2019 , 30 ( 3 ): 447 - 455 . DOI: 10.1093/annonc/mdy542 http://doi.org/10.1093/annonc/mdy542 https://linkinghub.elsevier.com/retrieve/pii/S0923753419310774 https://linkinghub.elsevier.com/retrieve/pii/S0923753419310774

DEKKERS J F , VAN VLIET E J , SACHS N , et al . Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids [J ] . Nat Protoc , 2021 , 16 ( 4 ): 1936 - 1965 . DOI: 10.1038/s41596-020-00474-1 http://doi.org/10.1038/s41596-020-00474-1 https://doi.org/10.1038/s41596-020-00474-1 https://doi.org/10.1038/s41596-020-00474-1

MAENHOUDT N , DEFRAYE C , BORETTO M , et al . Developing organoids from ovarian cancer as experimental and preclinical models [J ] . Stem Cell Reports , 2020 , 14 ( 4 ): 717 - 729 . DOI: 10.1016/j.stemcr.2020.03.004 http://doi.org/10.1016/j.stemcr.2020.03.004 https://linkinghub.elsevier.com/retrieve/pii/S2213671120300941 https://linkinghub.elsevier.com/retrieve/pii/S2213671120300941

HU Y W , SUI X Z , SONG F , et al . Lung cancer organoids analyzed on microwell arrays predict drug responses of patients within a week [J ] . Nat Commun , 2021 , 12 ( 1 ): 2581 . DOI: 10.1038/s41467-021-22676-1 http://doi.org/10.1038/s41467-021-22676-1 https://doi.org/10.1038/s41467-021-22676-1 https://doi.org/10.1038/s41467-021-22676-1

WANG Y C , JEON H . 3D cell cultures toward quantitative high-throughput drug screening [J ] . Trends Pharmacol Sci , 2022 , 43 ( 7 ): 569 - 581 . DOI: 10.1016/j.tips.2022.03.014 http://doi.org/10.1016/j.tips.2022.03.014 https://linkinghub.elsevier.com/retrieve/pii/S0165614722000797 https://linkinghub.elsevier.com/retrieve/pii/S0165614722000797

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