白介素8的3种亚型对卵巢癌SKOV<sub>3</sub>细胞生物学活性的影响

王爱萍; 王琪; 张玮; 尹巧云; 李力

doi:10.3969/j.issn.1007-3969.2013.04.002

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白介素8的3种亚型对卵巢癌SKOV₃细胞生物学活性的影响

更新时间：2025-12-31

- 白介素8的3种亚型对卵巢癌SKOV₃细胞生物学活性的影响
- The impact of three subtypes of IL-8 on biological functions of ovarian cancer SKOV₃cells
- 中国癌症杂志 2013年23卷第4期页码：248-253
- 作者机构：
  
  广西医科大学肿瘤医院妇瘤科，广西区域高发肿瘤重点实验室,广西,南宁,530021
- 作者简介：
- 基金信息：
  
  国家自然科学基金(No：30760266)
- DOI：10.3969/j.issn.1007-3969.2013.04.002
  中图分类号：
- 网络出版：2014-11-19，
  
  纸质出版：2013
- 稿件说明：
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王爱萍,王琪,张玮,尹巧云,李力. 白介素8的3种亚型对卵巢癌SKOV

王爱萍, 王琪, 张玮, et al. The impact of three subtypes of IL-8 on biological functions of ovarian cancer SKOV₃cells[J]. China Oncology, 2013, 23(4): 248-253.
王爱萍,王琪,张玮,尹巧云,李力. 白介素8的3种亚型对卵巢癌SKOV DOI： 10.3969/j.issn.1007-3969.2013.04.002.

王爱萍, 王琪, 张玮, et al. The impact of three subtypes of IL-8 on biological functions of ovarian cancer SKOV₃cells[J]. China Oncology, 2013, 23(4): 248-253. DOI： 10.3969/j.issn.1007-3969.2013.04.002.

摘要

背景与目的：卵巢癌的进展是一种多因素、多环节、多阶段的过程，近来研究表明卵巢癌患者白介素8(IL-8)水平显著高于良性肿瘤患者及健康人群，卵巢癌的生长和转移可能与过度表达的IL-8有关，但尚无报道表明IL-8主要亚型在卵巢癌中的作用。本研究探讨IL-8的3种亚型IL-8(1-77)、IL-8(5-77)和IL-8(9-77)对卵巢癌SKOV3细胞的增殖、生长、迁移和侵袭能力的影响及其作用机理。方法：确认分别获得IL-8的3种亚型稳定转染的SKOV3细胞后，应用MTT比色测定法比较各细胞的生长曲线；采用流式细胞仪比较各细胞的细胞生长周期；采用集落形成实验比较各组细胞的克隆率；运用Transwell小室比较各细胞体外迁移和侵袭能力。结果：IL-8(1-77)-pwpi-SKOV3细胞和IL-8(5-77)-pwpi-SKOV3在G2期的比率明显高于SKOV3(P=0.017，P=0.020)；IL-8(1-77)-pwpi-SKOV3和IL-8(5-77)-pwpi-SKOV3细胞克隆率明显高于SKOV3(P=0.000，P=0.001)，IL-8(9-77)-pwpi-SKOV3细胞克隆率明显低于SKOV3(P=0.013)；细胞体外迁移能力多组间差异无统计学意义(P0.05)，但细胞体外侵袭能力IL-8(1-77)-pwpi-SKOV3和IL-8(5-77)-pwpi-SKOV3明显强于SKOV3(P=0.000，P=0.002)，IL-8(9-77)-pwpi-SKOV3明显弱于SKOV3(P=0.067)。结论：IL-8(1-77)和IL-8(5-77)可能促进卵巢癌SKOV3细胞的生长和增殖，并增强其侵袭能力，而IL-8(9-77)对卵巢癌SKOV3细胞增殖可能有抑制作用。

Abstract

Background and purpose: Ovarian cancer progression is a multistep process. Recent research shows that IL-8 level of ovarian cancer patients is significantly higher than that of patients with benign tumors and normal people

the growth and metastasis of ovarian cancer may be associated with over-expression of IL-8

but there is no report about the function of the main subtypes of IL-8 in ovarian cancer. The study was to investigate the influence of IL-8 three subtypes on ovarian cancer SKOV3 cell proliferation

growth

migrating and invasive capability

and the underlying mechanism. Methods: After confirming obtaining SKOV3 cell with three hypotypes IL-8(1-77)

IL-8(5-77)

IL-8(9-77) over-expression respectively

MTT colorimetric method was used to detect the growth cure of different cells; The flow cytometry was used to compare the cell cycle of different cells; The cell proliferation capability of different cells was tested by the cell body colony formation; Transwell closet was used to compare different cell body’s external migration and invasive capability. Results: IL-8(1-77)-pwpi-SKOV3 and IL-8(5-77)-pwpi-SKOV3 cells in the ratio of G2 phase were significantly higher than that of SKOV3 cells (P=0.017 and 0.020). The cloning formation rate of IL-8(1-77)-pwpi-SKOV3 and IL-8 (5-77) -pwpi-SKOV3 cells were significantly higher than that of SKOV3 cells (P=0.000 and 0.001)

IL-8 (9-77)-pwpi-SKOV3 cells was significantly lower than SKOV3 cells (P=0.013); the migration ability in vitro of different groups of cells had no statistically significant differences (P0.05)

but the invasion ability in vitro of IL-8( 1-77 )-pwpi-SKOV3 and IL-8 (5-77) -pwpi-SKOV3 cells were significantly stronger than that of SKOV3 cells(P=0.000 and 0.002)

IL-8 (9-77) -pwpi-SKOV3 cells was significantly weaker than that of SKOV3 cells(P=0.067). Conclusion: The subtypes of IL-8 (1-77) and IL-8 (5-77) can promote ovarian cancer SKOV3 cell growth and proliferation

and enhance their invasive ability

while IL-8(9-77) inhibits proliferation of ovarian cancer SKOV3 cells.

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白介素8的3种亚型对卵巢癌SKOV3细胞生物学活性的影响

The impact of three subtypes of IL-8 on biological functions of ovarian cancer SKOV3cells

DOI：10.3969/j.issn.1007-3969.2013.04.002

摘要

Abstract

关键词

Keywords

references

白介素8的3种亚型对卵巢癌SKOV₃细胞生物学活性的影响

The impact of three subtypes of IL-8 on biological functions of ovarian cancer SKOV₃cells