Background and purpose: Multi-drug resistance is a major reason for the chemotherapy failure of human ovarian cancer. Sorcin was found overexpression in drug resistance tumors and it may be the target of multidrug resistance reversal. The present article was aimed to study the effect and mechanism of Sorcin silencing on drug resistance of human ovarian cancer SKOV3/CDDP cell lines. Methods: The stable Sorcin silencing SKOV3/CDDP cell lines were established. MTS assay

flow cytometry was used to analyze the intra-cellular Rh-123 content

cells apoptosis and cycle. Real-time

Western blot and report gene assay were used to analyze the expression changes of genes

including MDR1

MPR1

Survivin

Bcl-2

p-Akt and NF-κB. Results: Sorcin inhibition enhanced the drug sensitivity of SKOV3/CDDP cells

increased the intra-cellular Rh-123 content and cell apoptosis

and arrested cell cycle in G2/M.The protein levels of MDR1

MPR1

Survivin

Bcl-2

p-Akt were down-regulated

the mRNA levels of MDR1 of Sh-Sorcin-1 and Sh-Corcin-2 group were decreased to 42.3% and 26.5% of untransfected control

MRP1 were decreased to 33.2% and 18.9% of untransfected control

Survivin were decreased to 36.2% and 29.6% of untransfected control

Bcl-2 were decreased to 54.6% and 46.7% of untransfected control

transcriptional activity of NF-κB were decreased to 56.3% and 38.4% of untransfected control respectively inSKOV3/CDDP cell lines after Sorcin silence. Conclusion: Sorcin silencing could reverse SKOV3/CDDP drug resistance and enhance drug sensitivity

which involves the decreased phosphorylation level of Akt and transcriptional activity of NF-κB.