Background and purpose: Actin related protein 2/3 complex subunit 5 (ARPC5) is involved in the packaging of actin

and then affects the mobility of cells. It plays an important role in the formation of invadopodia of cancer cells. This study aimed to investigate the effect of ARPC5 gene-silence on cell proliferation

migration and invasion of lung squamous carcinoma cell line SK-MES-1. Methods: Under the induction of LipofectamineTM RNAiMAX

the recombinant of si-ARPC5 was transfected into SK-MES-1. The experiment set up 3 groups: blank (mock) group

negative control (NC) group and si-ARPC5 transfected group. Cell proliferation

migration and invasion assays were investigated by cell counting kit(CCK-8) method

scratch assay and Transwell Chambers respectively. Results: The result of qRT-PCR and Western blot displayed downregulation of ARPC5 in mRNA and protein level of SK-MES-1 cell. Proliferation rate of SK-MES-1 cells in 72 h after si-ARPC5 transfection was lower than those in mock and NC groups (t=7.993

t=8.681

P0.05). Compared to the mock and NC groups

the scratch repairing rate of the si-ARPC5 group was lower [(43.32±0.23)% vs (73.11±0.43)% and (76.58±0.88)%] (t=7.348

t=10.614

P0.05); And the number of penetrating the membrane was significantly reduced [(27±6) vs (101±11) and (92±9)] (t=10.229

t=8.391

P0.05). Conclusion: Downregulation of ARPC5 gene can inhibit the proliferation

migration

and invasion of SKMES-1 cell.