Background and purpose: Breast cancer as one of the most common malignant tumor among women in China

it accounts for 12.2% of all newly diagnosed breast cancers and 9.6% of all deaths from breast cancer worldwide. The aim of this study was to investigate the relationship between single nucleotide polymorphisms(SNPs) in 2q35 rs13387042 and 8q24 rs13281615 and the risk of breast cancer in Han premenopausal women of Northern China. Methods: 280 patients with breast cancer and 287 healthy controls in premenopausal state were genotyped for SNP 2q35 rs13387042 and 8q24 rs13281615 by the SNaPshot method

and compared the different genotypes and alleles with relation to breast cancer risk. Results: Differences of 2q35 rs13387042 genotype frequencies between breast cancer and control were significantly different (P=0.017). No statistically significant difference of 8q24 rs13281615 genotype frequencies between breast cancers and controls was found (P=0.967). The results of logistic regression showed that the carriers of GA genotype and GA+ AA genotype increased risk for breast cancer compared to the carriers with 2q35 rs13387042 GG genotype(OR=1.793

95%CI: 1.177-2.733

P=0.007；OR=1.691

95%CI: 1.122-2.550

P=0.012)

but not the carriers of AA genotype; Compared with G allele

A allele significantly increased the risk of breast cancer(OR=1.505

95%CI: 1.033-2.193

P=0.033). The carriers of AG genotype or GG genotype or AG+GG genotype did not confer risk for breast cancer compared to the carriers with 8q24 rs13281615 AA genotype(OR=0.992

95%CI: 0.660-1.490

P=0.968; OR=1.047

95%CI: 0.642-1.708

P=0.853; OR=1.007

95%CI: 0.682-1.487

P=0.971); Compared with A allele

G allele did not increase the risk of breast cancer(OR=1.021

95%CI: 0.809-1.288

P=0.863). Conclusion: This experiment verified that 2q35 rs13387042 polymorphism site increased risk of breast cancer susceptibility among Han premenopausal women of Northern China. There was not any significant association between 8q24 rs13281615 polymorphism site and breast cancer susceptibility among Han premenopausal women of Northern China under the current sampling scale.