ABCC4/MRP4和ABCC5/MRP5基因在NK/T细胞淋巴瘤的表达及与临床疗效的关系

doi:10.3969/j.issn.1007-3969.2014.01.002

中国癌症杂志 ›› 2014, Vol. 24 ›› Issue (1): 8-14.doi: 10.3969/j.issn.1007-3969.2014.01.002

ABCC4/MRP4和ABCC5/MRP5基因在NK/T细胞淋巴瘤的表达及与临床疗效的关系

李汝平¹,韩丽娟¹,张旭东¹,张明智¹,胡腾鹏¹,秦贝贝¹,文建国²

1.郑州大学第一附属医院肿瘤科，河南郑州 450052 ；
2.河南省高等学校临床医学重点学科开放实验室，河南郑州 450052

出版日期:2014-01-30 发布日期:2014-02-14
通信作者: 张明智　E-mail:mingzhi_zhang@126.com

The expression of ABCC4/MRP4 and ABCC5/MRP5 gene in the NK/T cell lymphoma and its relationship with clinical efficacy

LI Ru-ping¹,HAN Li-juan¹,ZHANG Xu-dong¹,ZHANG Ming-zhi¹,HU Teng-teng¹,QIN Bei-bei¹,WEN Jian-guo²

1.Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou Henan 450052, China;
2.Institute of Clinical Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou Henan 450052, China

Published:2014-01-30 Online:2014-02-14
Contact: ZHANG Ming-zhi E-mail: mingzhi_zhang@126.com

摘要/Abstract

摘要： 背景与目的：NK/T细胞淋巴瘤(natural killer/T cell lymphoma)疗效差，多药耐药(multidrug resistance，MDR)的产生是引起肿瘤产生耐药和化疗疗效降低及失败的原因之一，本文通过研究多药耐药相关蛋白4(ABCC4 multidrug resistance associated protein，ABCC4/MRP)基因和多药耐药相关蛋白5(ABCC5 multidrug resistance associated protein，ABCC5/MRP)基因在NK/T细胞淋巴瘤SNK-6、YTS细胞株和组织中的表达情况，结合临床疗效了解其与预后的关系。方法：应用实时荧光定量PCR(real-time PCR)技术和免疫组织化学法(immunohistochemistry，IHC)检测ABCC4/MRP4、ABCC5/MRP5基因mRNA和蛋白表达水平。结果：与正常NK细胞相比，ABCC4/MRP4、ABCC5/MRP5基因在SNK-6、YTS细胞株中高表达(P<0.05)；与鼻炎组织相比，ABCC4/MRP4、ABCC5/MRP5基因在NK/T细胞淋巴瘤患者组织中高表达(P<0.05)；ABCC4/MRP4、ABCC5/MRP5基因表达量的高低和临床疗效呈负相关(P<0.05)。结论：ABCC4/MRP4、ABCC5/MRP5基因及其表达蛋白影响NK/T细胞淋巴瘤的疗效。

Abstract: Background and purpose: Natural killer/T cell lymphoma in poor effects, the production of multidrug resistance is one of the reasons to reduce the chemotherapy effect or failure. This study aimed to discuss the multidrug resistance associated protein 4 (ABCC4/MRP4) gene and multidrug resistance associated protein 5 (ABCC5/MRP5) gene expression in NK/T cell lymphoma SNK-6, YTS cell lines and NK/T cell lymphoma tissues, and the relationship between the level of ABCC4/MRP4, ABCC5/MRP5 gene expression and the clinical efficacy. Methods: Real-time fluorescence quantitative PCR (Real time-PCR) and immunohistochemical method (IHC) were used to detect the ABCC4/MRP4, ABCC5/MRP5 gene and protein expression. Results: Compared with the normal NK cells, ABCC4/MRP4 and ABCC5/MRP5 gene in SNK-6, YTS cell lines were highly expressed (P<0.05); Compared with rhinitis tissues, the expression of ABCC4/MRP4, ABCC5/MRP5 gene was higher in the NK/T cell lymphoma tissues (P<0.05); The expression level of ABCC4/MRP4 and ABCC5/MRP5 gene was negative correlation with clinical efficacy (P<0.05). Conclusion: The expression of ABCC4/MRP4 and ABCC5/MRP5 gene affects the of clinical efficacy of NK/T cell lymphoma.

Key words: ABCC4/MRP4 gene, ABCC5/MRP5 gene, NK/T cell lymphoma, SNK-6/YTS cell lines

李汝平,韩丽娟,张旭东,张明智,胡腾鹏,秦贝贝,文建国. ABCC4/MRP4和ABCC5/MRP5基因在NK/T细胞淋巴瘤的表达及与临床疗效的关系[J]. 中国癌症杂志, 2014, 24(1): 8-14.

LIRu-ping,HANLi-juan,ZHANGXu-dong,ZHANGMing-zhi,HUTeng-teng,QINBei-bei,WENJian-guo. The expression of ABCC4/MRP4 and ABCC5/MRP5 gene in the NK/T cell lymphoma and its relationship with clinical efficacy[J]. China Oncology, 2014, 24(1): 8-14.

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ABCC4/MRP4和ABCC5/MRP5基因在NK/T细胞淋巴瘤的表达及与临床疗效的关系

The expression of ABCC4/MRP4 and ABCC5/MRP5 gene in the NK/T cell lymphoma and its relationship with clinical efficacy

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