4种人肾细胞癌原位移植裸鼠模型建立方法的比较

doi:10.19401/j.cnki.1007-3639.2017.03.003

中国癌症杂志 ›› 2017, Vol. 27 ›› Issue (3): 177-185.doi: 10.19401/j.cnki.1007-3639.2017.03.003

4种人肾细胞癌原位移植裸鼠模型建立方法的比较

赵佩佩^1,2，陈晓静²，王巧玲^1,2，赵　雪²，赵亚男²，刘培峰²，戴慧莉^1,2

1. 上海交通大学医学院附属仁济医院肾脏科，上海200127 ；
2. 上海交通大学医学院附属仁济医院中心实验室，上海200127

出版日期:2017-03-30 发布日期:2017-04-12
通信作者: 戴慧莉　E-mail: dhl_sh@163.com
基金资助:
上海市科学技术委员会科研计划项目(15140902700)。

A comparative study of four methods of establishing orthotopic human renal cell carcinoma models in nude mice

ZHAO Peipei^1,2, CHEN Xiaojing², WANG Qiaoling^1,2, ZHAO Xue², ZHAO Yanan², LIU Peifeng², DAI Huili^1,2

1. Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; 2. Department of Central Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China

Published:2017-03-30 Online:2017-04-12
Contact: DAI Huili E-mail: dhl_sh@163.com

摘要/Abstract

摘要： 背景与目的：肾细胞癌是最常见的肾脏恶性肿瘤，起病隐匿，恶性程度高。研究旨在建立成功率高、稳定的人肾细胞癌原位动物造模方法。方法：采用人肾细胞癌细胞(786-0、ACHN)进行细胞悬液原位注射法、皮下成瘤后引瘤原代细胞悬液原位注射法、皮下成瘤后引瘤组织块原位移植肾包膜下法及皮下成瘤后引瘤组织块原位移植肾周筋膜内法建立原位移植裸鼠模型，采用PET/CT、H-E染色、免疫组织化学染色及血清生化检测等手段观察成瘤率及肿瘤生长情况，评估4种方法的建模效果。结果：人肾细胞癌皮下移植瘤裸鼠模型，ACHN组成瘤率(90%)高于786-0组(30%)；人肾细胞癌原位移植裸鼠模型，786-0原位注射组、ACHN原位注射组、ACHN皮下移植瘤引瘤后原代细胞原位注射组、ACHN组织块肾包膜下包埋组和ACHN组织块肾周筋膜内包埋组的成瘤率分别为33%、80%、90%、100%和20%，其中以ACHN皮下成瘤后引瘤组织块原位移植肾包膜下法成瘤率最高，影像学及病理组织学检查结果显示符合低分化肾细胞癌。结论：成功建立4种人肾细胞癌原位移植裸鼠模型，其中以ACHN皮下成瘤后引瘤组织块原位移植肾包膜下法成瘤效果最佳，为肾细胞癌发病机制及靶向治疗的进一步研究提供理想的动物模型。

关键词: 肾细胞癌, 裸鼠, 原位移植, PET/CT, ACHN细胞

Abstract: Background and purpose: Renal cell carcinoma is the most common form of kidney cancer, characterized by lack of early symptoms and high malignancy. This study aimed to establish orthotopic nude mice models of human renal cell carcinoma with high success rate and good repeatability. Methods: The four types of methods which were adopted to establish the orthotopic models of renal cell carcinoma were orthotopic injection of 786-0 and ACHN cell suspensions, orthotopic injection of primary cell suspensions obtained from the subcutaneous tumor tissues, renal subcutis orthotopic implantation into renal capsule and surgical subcutis orthotopic implantation into renal fascia. To gain insights into the tumorigenicity and the growth of transplantation tumors, the imageological examination (PET/CT), histological examination (H-E staining, immunohistochemistry staining) and biochemical analysis of blood were carried out. Results: In terms of the subcutaneous transplantation of human renal cell carcinoma models in nude mice, tumorigenic rate of ACHN cells (90%) was higher than that of 786-0 cells (30%). The tumorigenic incidences of 786-0 cell suspensions orthotopic injection, ACHN cell suspensions orthotopic injection, ACHN subcutis cellular suspensions orthotopic injection, ACHN subcutis orthotopic implantation into renal capsule and renal fascia were 33%, 80%, 90%, 100% and 20%, respectively. ACHN subcutis orthotopic implantation into renal capsule was the most effective approach. Imageological and histological results accorded with poorly differentiated renal cell carcinoma. Conclusion: Four orthotopic nude mice models of human renal cell carcinoma were successfully established. Among these methods, ACHN subcutis orthotopic implantation into renal capsule is the most effective approach, which provides an ideal model for the research on biological behavior of human renal cell carcinoma and its treatment.

Key words: Renal cell carcinoma, Nude mice, Orthotopic transplantation, PET/CT, ACHN cells

赵佩佩,陈晓静,王巧玲,等. 4种人肾细胞癌原位移植裸鼠模型建立方法的比较[J]. 中国癌症杂志, 2017, 27(3): 177-185.

ZHAO Peipei, CHEN Xiaojing, WANG Qiaoling, et al. A comparative study of four methods of establishing orthotopic human renal cell carcinoma models in nude mice[J]. China Oncology, 2017, 27(3): 177-185.

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4种人肾细胞癌原位移植裸鼠模型建立方法的比较

A comparative study of four methods of establishing orthotopic human renal cell carcinoma models in nude mice

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