关键词: 卵巢上皮癌, IL-8亚型, 生物学功能

Abstract:

Background and purpose: Ovarian cancer progression is a multistep process. Recent research shows that IL-8 level of ovarian cancer patients is significantly higher than that of patients with benign tumors and normal people, the growth and metastasis of ovarian cancer may be associated with over-expression of IL-8, but there is no report about the function of the main subtypes of IL-8 in ovarian cancer. The study was to investigate the influence of IL-8 three subtypes on ovarian cancer SKOV3 cell proliferation, growth, migrating and invasive capability, and the underlying mechanism. Methods: After confirming obtaining SKOV3 cell with three hypotypes IL-8(1-77), IL-8(5-77), IL-8(9-77) over-expression respectively, MTT colorimetric method was used to detect the growth cure of different cells; The flow cytometry was used to compare the cell cycle of different cells; The cell proliferation capability of different cells was tested by the cell body colony formation; Transwell closet was used to compare different cell body’s external migration and invasive capability. Results: IL-8(1-77)-pwpi-SKOV3 and IL-8(5-77)-pwpi-SKOV3 cells in the ratio of G2 phase were significantly higher than that of SKOV3 cells (P=0.017 and 0.020). The cloning formation rate of IL-8(1-77)-pwpi-SKOV3 and IL-8 (5-77) -pwpi-SKOV3 cells were significantly higher than that of SKOV3 cells (P=0.000 and 0.001), IL-8 (9-77)-pwpi-SKOV3 cells was significantly lower than SKOV3 cells (P=0.013); the migration ability in vitro of different groups of cells had no statistically significant differences (P>0.05), but the invasion ability in vitro of IL-8( 1-77 )-pwpi-SKOV3 and IL-8 (5-77) -pwpi-SKOV3 cells were significantly stronger than that of SKOV3 cells(P=0.000 and 0.002), IL-8 (9-77) -pwpi-SKOV3 cells was significantly weaker than that of SKOV3 cells(P=0.067). Conclusion: The subtypes of IL-8 (1-77) and IL-8 (5-77) can promote ovarian cancer SKOV3 cell growth and proliferation, and enhance their invasive ability, while IL-8(9-77) inhibits proliferation of ovarian cancer SKOV3 cells.

Key words: Ovarian cancer, IL-8 subtype, Biological functions