Hes1在结肠癌组织中的表达及对SW620细胞成瘤能力的影响

doi:10.3969/j.issn.1007-3969.2014.09.002

中国癌症杂志 ›› 2014, Vol. 24 ›› Issue (9): 646-651.doi: 10.3969/j.issn.1007-3969.2014.09.002

Hes1在结肠癌组织中的表达及对SW620细胞成瘤能力的影响

杨妙玲,高飞^*

暨南大学附属第一医院消化内科，广东广州 510630

出版日期:2014-09-30 发布日期:2014-11-12
通信作者: *：原工作单位为南方医科大学。高飞　E-mail:gaofeidoc@163.com
基金资助:
国家自然科学基金青年项目(No：81401973)；广东省医学科研基金(No：B2014222)；中央高校基本科研业务费专项资金(No：21614304)

Increased expression of Hes1 and its effect on tumour-formation ability in colon cancer

YANG Miao-ling, GAO Fei^*

Department of Gastroenterology, the First Affiliated Hospital of Jinan University, Guangzhou Guangdong 510630, China

Published:2014-09-30 Online:2014-11-12
Contact: GAO Fei E-mail: gaofeidoc@163.com

摘要/Abstract

摘要：

背景与目的：研究发现Hes1可促进多种肿瘤的发生、发展和侵袭转移，且Hes1过表达可能会导致结肠癌的发生。本研究探讨Hes1在结肠癌组织中的表达及其对结肠癌SW620细胞株成瘤能力的影响。方法：免疫组化和定量逆转录聚合酶链反应(quantitative reverse transcription-polymerase chain reaction，qRT-PCR)检测Hes1在结肠癌组织中的表达并分析其表达与结肠癌分化程度的关系；建立Hes1过表达的结肠癌SW620细胞株，qRT-PCR和蛋白质印迹法(Western blot)分别检测Hes1在mRNA水平和蛋白水平的表达，并在裸鼠体内检测Hes1对SW620细胞皮下成瘤能力的影响。结果：Hes1在正常结肠组织中少许表达，表达多位于结肠腺管的底部。然而Hes1在结肠癌组织中的表达显著高于癌旁正常结肠组织，且Hes1的表达水平与细胞分化程度呈负相关(P<0.05)；在结肠癌SW620细胞株中稳定转染Hes1后，Hes1被成功过表达，约380倍(P<0.05)；用1×10⁵个Hes1过表达的SW620细胞及对照细胞株分别注射至裸鼠皮下，结果Hes1过表达的SW620细胞成瘤能力增强，瘤体较对照组大，生长速度较对照组快。结论：Hes1在结肠癌组织中的表达显著高于癌旁正常结肠组织，并且随结肠癌组织分化程度降低而表达量上调；Hes1在体内促进结肠癌SW620细胞的成瘤能力。

关键词: Hes1, 结肠癌, 成瘤能力

Abstract:

Background and purpose: It is reported that Hes1 is related to the progression and metastasis of many kinds of tumor. This study was to investigate the expression of Hes1 in colon cancer and its effect on tumourformation ability of SW620 cells. Methods: The expression of Hes1 in colon cancer tissues and control normal samples was analyzed by immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (qRTPCR), and the relationship between Hes1 expression and differentiation in human colon cancer was detected. Hes1 overexpressing SW620 cell lines were established, and the expression of Hes1 mRNA and protein was detected by qRTPCR and Western blot simultaneously. We also detected the effect of Hes1 on the ability of tumour-formation in Hes1 overexpressing SW620 cells in nude mice in vivo. Results: We found that Hes1 was expressed in almost all normal tissues, particularly at the bottom of the crypts, and in all cancer tissues, including moderate and poorly differentiated cancer samples and well-differentiated cancer samples. In addition, the expression of Hes1 in poorly differentiated cancer samples was higher than that observed in well-differentiated tumor (P<0.05). After stably transfected Hes1 in SW620 colon cancer cell lines, Hes1 was overexpressed successfully (P<0.05). We injected 1×105 Hes1-overexpressing colon cancer cells and the control cells into nude mice subcutaneously, and found Hes1-overexpressing tumours exhibited significantly bigger size compared with that observed in controls. The growth of the Hes1-overexpressing tumours was found to be slightly faster than those of the controls. Conclusion: Hes1 is overexpressed in colon cancer than that in normal tissue, and Hes1 is upregulated in poorly differentiated cancer samples compared with welldifferentiated tumour samples. Hes1 has a positive influence on the ability of tumour-formation in SW620 cells in vivo.

Key words: Hes1, Colon cancer, Tumor-formation ability

杨妙玲,高飞. Hes1在结肠癌组织中的表达及对SW620细胞成瘤能力的影响[J]. 中国癌症杂志, 2014, 24(9): 646-651.

YANG Miao-ling, GAO Fei. Increased expression of Hes1 and its effect on tumour-formation ability in colon cancer[J]. China Oncology, 2014, 24(9): 646-651.

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Hes1在结肠癌组织中的表达及对SW620细胞成瘤能力的影响

Increased expression of Hes1 and its effect on tumour-formation ability in colon cancer

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