内质网分子蛋白在小鼠腹水型肝癌高、低淋巴道转移株中的表达及意义

doi:10.3969/j.issn.1007-3969.2015.02.003

中国癌症杂志 ›› 2015, Vol. 25 ›› Issue (2): 95-98.doi: 10.3969/j.issn.1007-3969.2015.02.003

内质网分子蛋白在小鼠腹水型肝癌高、低淋巴道转移株中的表达及意义

张亚楠^1，2，唐建武¹

1. 大连医科大学基础医学院病理学与法医学教研室，辽宁大连 116044 ；
2. 山东中医药大学基础医学院病理学教研室，山东济南 250355

出版日期:2015-02-28 发布日期:2015-05-13
通信作者: 唐建武　E-mail:jianwutang@163.com
基金资助:
国家自然科学基金面上项目(30572098)。

The expression and significance of ERp29 in 2 mice hepatocellular carcinoma ascites syngeneic cell lines with high and low lymph node metastasis rates

ZHANG Yanan^1,2, TANG Jianwu¹

1.Department of Pathology and Forensic Medicine, Dalian Medical University, Dalian Liaoning 116044, China; 2.Department of Pathology, Shandong University of Traditional Chinese Medicine, Jinan Shandong 250355, China

Published:2015-02-28 Online:2015-05-13
Contact: TANG Jianwu E-mail: jianwutang@163.com

摘要/Abstract

摘要：
　背景与目的：内质网分子伴侣29(endoplasmic reticulum molecular chaperons 29，ERp29)是一种内质网应激蛋白，其高表达与肿瘤转移相关。本研究旨在探讨ERp29在小鼠腹水型肝癌高、低淋巴道转移株中的表达及与肝癌淋巴道转移的关系。方法：构建小鼠腹水型肝癌细胞株(高转移株Hca-F、低转移株Hca-P)淋巴道转移动物模型，通过免疫组织化学、蛋白质印迹法(Western blot)和流式细胞仪等方法检测ERp29在Hca-F、Hca-P细胞株中的表达情况。结果：免疫组织化学检测结果显示，ERp29在Hca-F、Hca-P中均呈阳性表达，定位于细胞质，部分细胞核也有表达。Western blot检测结果显示，ERp29在Hca-F、Hca-P中的表达吸光度值分别为0.97±0.03和1.17±0.03，ERp29在Hca-F细胞株中的表达(吸光度值)明显低于Hca-P，差异有统计学意义(P＜0.01)。流式细胞仪检测结果显示，ERp29在Hca-F细胞株中的相对荧光强度值(375.27±47.33)显著低于Hca-P(623.91±46.80)，差异有统计学意义(P＜0.01)。结论：ERp29在小鼠腹水型肝癌细胞株Hca-F和Hca-P均有表达，且在Hca-F中表达较低，而在Hca-P中表达较高。ERp29可能与肝癌淋巴道转移密切相关。

关键词: 　内质网分子伴侣29, 肝癌, 淋巴道转移

Abstract:
Background and purpose: ERp29 belongs to endoplasmic reticulum stress proteins and might play roles in neoplasm metastasis. This study aimed to investigate the expression of ERp29 in Hca-F and Hca-P cells and to elucidate its role in lymphatic metastasis. Methods: Immunohistochemistry, Western blot and flow cytometry analysis were used to detect the expression of ERp29 in Hca-F and Hca-P. Results: The results of immunohistochemistry suggested that ERp29 protein was located at cytoplasm of hepatic cells and some were also detected in the nucleus. The results of western blot suggested that ERp29 had positive expression in Hca-F and Hca-P cells. Its expression in Hca-F cells was apparently lower than that in Hca-P cells. And there was statically different between Hca-F and Hca-P cells (P<0.01). The relative fluorescence intensity of ERp29 protein was significantly lower in Hca-F cells (375.27±47.33) than that in Hca-F cells (623.91±46.80) by flow cytometry (P<0.01). Conclusion: The different expression of ERp29 may be related to the potential ability of tumor lymphatic metastasis in Hca-F and Hca-P cells.

Key words: Endoplasmic reticulum molecular chaperons 29, Hepatocellular carcinoma, Lymphatic metastasis

张亚楠,唐建武. 内质网分子蛋白在小鼠腹水型肝癌高、低淋巴道转移株中的表达及意义[J]. 中国癌症杂志, 2015, 25(2): 95-98.

ZHANG Yanan, TANG Jianwu. The expression and significance of ERp29 in 2 mice hepatocellular carcinoma ascites syngeneic cell lines with high and low lymph node metastasis rates[J]. China Oncology, 2015, 25(2): 95-98.

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内质网分子蛋白在小鼠腹水型肝癌高、低淋巴道转移株中的表达及意义

The expression and significance of ERp29 in 2 mice hepatocellular carcinoma ascites syngeneic cell lines with high and low lymph node metastasis rates

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