颗粒蛋白前体调控胃癌细胞增殖与衰老的效应研究

doi:10.3969/j.issn.1007-3969.2015.03.003

中国癌症杂志 ›› 2015, Vol. 25 ›› Issue (3): 173-178.doi: 10.3969/j.issn.1007-3969.2015.03.003

颗粒蛋白前体调控胃癌细胞增殖与衰老的效应研究

李媛媛¹，王红艳^2,3，吴晓燕²，宋瑞卉¹，李敬⁴

1. 潍坊医学院形态学实验室，山东潍坊261053 ；
2. 潍坊医学院病原生物学教研室，山东潍坊261053 ；
3. 山东省高校免疫学重点实验室，山东潍坊261053 ；
4. 潍坊医学院基础医学院，山东潍坊261053

出版日期:2015-03-30 发布日期:2015-05-18
通信作者: 王红艳　E-mail:sdwfwhy@163.com
基金资助:
国家自然科学基金资助项目(81201262)；山东省医药卫生科技发展计划项目(2011HZ114)。

Effects of progranulin on proliferation and senescence in gastric cancer cells

LI Yuanyuan¹, WANG Hongyan^2,3, WU Xiaoyan², SONG Ruihui¹, LI Jing⁴

1.Department of Morphology, Weifang Medical University, Weifang Shandong 261053, China; 2.Department of Microbiology, Weifang Medical University,
Weifang Shandong 261053, China; 3.Key Lab for Immunology in Universities of Shandong Province, Weifang Shandong 261053, China; 4.Basic Medical College, Weifang Medical University, Weifang Shandong 261053, China

Published:2015-03-30 Online:2015-05-18
Contact: WANG Hongyan E-mail: sdwfwhy@163.com

摘要/Abstract

摘要： 背景与目的：颗粒蛋白前体(progranulin，PGRN)是一种新型生长因子，在细胞迁移、细胞周期进展及肿瘤形成过程中发挥着重要作用。PGRN在多种恶性肿瘤细胞中高表达，不仅参与肿瘤的生长过程，还与肿瘤的发生、演变过程关系密切。本研究旨在探讨PGRN在胃癌组织中的表达，及其对胃癌BGC823细胞增殖与衰老的影响。方法：利用免疫组化方法检测胃癌组织及癌旁组织中PGRN的表达；利用实时定量聚合酶链反应(quantitative real-time polymerase chain reaction，qRT-PCR)干扰胃癌细胞株BGC823中PGRN的表达；通过四甲基偶氮唑盐(MTT)法、细胞克隆形成和细胞衰老检测实验，探讨PGRN对BGC823细胞增殖与衰老的影响。结果：PGRN在胃癌组织中高表达。PGRN表达降低后，胃癌细胞的增殖与克隆形成能力均显著降低。PGRN-siRNA细胞的克隆形成率为(25.3±3.1)%，对照组细胞的克隆形成率为(72.1±5.7)%，正常组细胞的克隆形成率为(80.3±4.0)%。两两比较，对照组与正常组间差异无统计学意义(P>0.05)，实验组与其他两组间差异均有统计学意义(P均<0.05)。干扰PGRN表达能够明显促进BGC823细胞衰老。PGRN-siRNA细胞衰老阳性率为(27.6±2.1)%，对照组细胞衰老阳性率为(3.2±1.3)%，正常组细胞衰老阳性率为(1.9±1.2)%。两两比较，对照组与正常组间差异无统计学意义(P>0.05)，实验组与其他两组间差异均有统计学意义(P均<0.05)。结论：PGRN可作为新的胃癌标志物，为临床胃癌的靶向治疗提供新的思路。

关键词: 胃肿瘤, 颗粒蛋白前体, siRNA, 细胞衰老

Abstract: Background and purpose: Progranulin (PGRN) is a novel growth factor that plays an important role in the tumorigenicity, tumor cell migration and cell cycle. Its expression in many malignant tumor cells is high. It is not only involved in tumor cell growth, but also closely related with the occurrence and evolution of tumor. This study was to investigate the expression of PGRN in gastric cancer and the effects on proliferation and senescence in gastric cancer cell line BGC823. Methods: Immunohistochemical method was used to detect the expression of PGRN in gastric cancer tissues and adjacent normal tissues; Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of PGRN in PGRN-siRNA BGC823 cells; MTT method, cell colony formation and cell senescence experiments were used to explore the effects of PGRN on proliferation and senescence in BGC823 cell. Results: PGRN protein levels were high in gastric cancer tissues; Knocking down the PGRN gene in BGC823 decreased the proliferation and clonogenic capacity, cloning efficiency in PGRN-siRNA group was (25.3±3.1)%, in the control group was (72.1±5.7)%, and in the normal cells was (80.3±4.0)%, there was no significant difference between normal group and control group, but there were significant differences among PGRN-siRNA group and the other two groups (P<0.05); Knocking down the PGRN gene in BGC823 cells could promote cell senescence. The positive rate of aging in PGRN-siRNA group was (27.6±2.1)%, in the control group was (3.2±1.3)%, and in the normal group was (1.9±1.2)%, there was no significant difference between normal group and control group. But there were significant differences among PGRN-siRNA group and the other two groups (P<0.05). Conclusion: PGRN can be used as a new marker for gastric cancer, and provide new ideas to the treatment of gastric cancer.

Key words: Gastric neoplasms, Progranulin, siRNA, Cell senescence

李媛媛,王红艳,吴晓燕, 等. 颗粒蛋白前体调控胃癌细胞增殖与衰老的效应研究[J]. 中国癌症杂志, 2015, 25(3): 173-178.

LI Yuanyuan, WANG Hongyan, WU Xiaoyan, et al. Effects of progranulin on proliferation and senescence in gastric cancer cells[J]. China Oncology, 2015, 25(3): 173-178.

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颗粒蛋白前体调控胃癌细胞增殖与衰老的效应研究

Effects of progranulin on proliferation and senescence in gastric cancer cells

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