中国癌症杂志 ›› 2017, Vol. 27 ›› Issue (7): 521-526.doi: 10.19401/j.cnki.1007-3639.2017.07.001

• 论著 • 上一篇    下一篇

人胎盘部位滋养细胞肿瘤细胞系的建立及其鉴定

刘齐雨1,2,李 可1,2,胥 婧1,2,黄云柯1,2,赵晨燕3,罗建民4,李桂玲1,2, 康 玉1,2,徐丛剑1,2   

  1. 1. 复旦大学附属妇产科医院中西医结合科,上海 200011 ;
    2. 上海市女性生殖内分泌相关疾病重点实验室,上海 200011 ;
    3. 复旦大学附属妇产科医院病理科,上海 200011 ;
    4. 复旦大学生物医学影像研究中心,上海 200032
  • 出版日期:2017-07-30 发布日期:2017-08-16
  • 通信作者: 康 玉 E-mail:yukang@fudan.edu.cn
  • 基金资助:
    国家自然科学基金(81472423);上海市科委国际合作项目(14430723000)。

Establishment and identification of human placental site trophoblastic tumor cell line

LIU Qiyu1,2, LI Ke1,2, XU Jing1,2, HUANG Yunke1,2, ZHAO Chenyan3, LUO Jianmin4, LI Guiling1,2, KANG Yu1,2, XU Congjian1,2   

  1. 1. Department of Integrated Traditional Chinese and Western Medicine, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China; 2. Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai 200011, China; 3. Department of Pathology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China; 4. Center for Biomedical Imaging, Fudan University, Shanghai 200032, China
  • Published:2017-07-30 Online:2017-08-16
  • Contact: KANG Yu E-mail: yukang@fudan.edu.cn

摘要: 背景与目的:胎盘部位滋养细胞肿瘤(placental site trophoblastic tumor,PSTT)是一种特殊类型的妊娠滋养细胞肿瘤。该研究旨在建立人PSTT细胞系,鉴定其基本特性。方法:通过手术切除的PSTT组织标本原代培养,纯化并传代,建立人PSTT细胞系。鉴定方法包括:观察细胞形态,CCK-8法绘制细胞生长曲线,进行染色体核型分析,细胞与肿瘤组织短串重复序列(short tandem repeat,STR)分析,以及免疫组织化学染色。结果:建立稳定传代的人PSTT细胞系PSTT-1。PSTT-1细胞单层贴壁生长,失去接触抑制性,呈多突起的纺锤形结构。体外培养生长良好,每5 d传代1次,目前已传至30余代。染色体核型分析结果为正常女性核型:46,XX。PSTT-1与肿瘤组织STR分析结果高度匹配。PSTT-1细胞表达β-catenin、CD146、人绒毛膜促性腺激素(human chorionic gonadotrophin,hCG)、人胎盘生乳素(human placental lactogen,hPL)和Muc4。结论:该研究成功建立人PSTT细胞系PSTT-1,为PSTT的研究提供了体外实验模型。

关键词: PSTT, 细胞系, 原代培养

Abstract: Background and purpose: Placental site trophoblastic tumor (PSTT) is a special type of gestational trophoblastic tumor. It remains no general cell line for PSTT. The aim of this study was to establish and identify a human PSTT cell line. Methods: In this study, a PSTT cell line (PSTT-1) was derived from primary culture of a surgically resected PSTT tissue. We further identified the basic characteristics of PSTT-1, including cell morphology, cell growth curve, karyotype analysis, short tandem repeat (STR) profiling of PSTT-1 and its parental tumor, and immunohistochemistry analysis. Results: A human PSTT cell line named PSTT-1 was successfully established. PSTT-1 formed a monolayer growth and lost contact inhibition, and exhibited a pleomorphic morphology. PSTT-1 cells grew actively in vitro. PSTT-1 was passaged every 5 days, and maintained in culture for more than 30 passages. Karyotyping of PSTT-1 revealed normal female karyotype: 46, XX. STR profiling of PSTT-1 was nearly identical to its parental tumor. Immunohistochemistry analysis of PSTT-1 revealed expression of β-catenin, CD146, human chorionic gonadotropin (hCG), human placental lactogen (hPL) and Muc4. Conclusion: This study established a PSTT cell line, which could be useful for further research on PSTT.

Key words: Placental site trophoblastic tumor, Cell line, Primary culture