最新刊期
卷 26 ， 期 8 ， 2016
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- 摘要：Background and purpose: Stanniocalcin 1 (STC1) has been reported to be up-regulated in various cancer tissues, and related to malignancy degree of cancer. However, the molecular mechanism of STC1 in lung cancer cells is still not clear. This experiment aimed to investigate the effects of STC1 on cell cycle and apoptosis of lung cancer A549 cells. Methods: A549 cells were transfected with validated siRNA for STC1 A549-STC1-siRNA and a negative control vector RNA A549-Vector. The gene and protein expression of cell cycle-related genes, including CyclinA, CyclinB1, CyclinD1, CyclinE, CDK2 and CDK4, as well as apoptosis-inhibiting genes Bcl-2, Bcl-xl and apoptosis-inducing genes Caspase-3, Bax, Bak and Bid, were detected by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot. The cell cycle distribution was determined with flow cytometry. Terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) was used to detect cell apoptosis. Results: After transfection with STC1-siRNA, the gene and protein expression of CyclinA, CyclinB1, CyclinD1, CyclinE, CDK2 and CDK4 decreased significantly in A549 cells (P<0.05). The proportion of cells in G0/G1 phase significantly increased, whereas the proportion of cells in S phase and G2/M phase decreased (P<0.05). The cell cycle was blocked at G0/G1 phase. Furthermore, compared with that in A549-Vector, the gene and protein expression of Bcl-2 and Bcl-xl in A549-STC1-siRNA was reduced significantly (P<0.05), while the expression of apoptosis-inducing genes Caspase-3, Bax, Bak and Bid increased obviously (P<0.05). In addition, the percentage of apoptotic cells significantly increased in A549-STC1-siRNA compared with that in A549-Vector detected by TUNEL method. Conclusion: Down-regulation of STC1 by RNAi can block the cell cycle of A549 cells, inhibit cell proliferation, and promote cell apoptosis.关键词：Stanniocalcin l;RNA interference;Cell cycle;Apoptosis2|2272|0更新时间：2025-12-31
- 摘要：Background and purpose: The study has found that tumor necrosis factor-related apoptosis inducing ligand (TRAIL) can enhance the cytotoxic effect of chemotherapeutic drugs on tumor cells. The aim of our study was to investigate the effects of the tumor TRAIL and cisplatin combined application on the growth and apoptosis of human ovarian cancer cell lines SKOV3 and OVCAR3, and the possible mechanism. Methods: Under the combined application of cisplatin and TRAIL, MTT method and flow cytometry were used to detect the proliferation and apoptosis of SKOV3 of OVCAR3 cells; the mRNA expression levels of death receptors, DR4 and DR5, were detected by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR). At the same time, the protein expression levels of DR4 and DR5 were detected by Western blot. Results: SKOV3 and OVCAR3 cells were sensitive to TRAIL protein, and with the increasing of TRAIL protein concentration, cell growth inhibitory rate up to 64%. When the combination application of TRAIL and cisplatin, the inhibition rate of the two cells reached more than 92%, and the two drugs showed high synergistic effect, compared with the single drug group (P<0.05). Flowcytometry analysis indicated that the synergistic killing effect of TRAIL and cisplatin was mainly due to the cell apoptosis. RTFQ-PCR and Western blot detection results showed that the DR4 and DR5 were up-regulated under the combined application of TRAIL and cisplatin. Conclusion: In vitro, TRAIL and cisplatin combined application can significantly inhibit the proliferation of human ovarian cancer cells and induce tumor cell apoptosis. TRAIL can obviously enhance the sensitivity of cisplatin to tumor cells. The mechanism may be related to the increased death receptor DR4 and DR5 expression level.关键词：Ovarian cancer;Tumor necrosis factor-related apoptosis inducing ligand;Cisplatin;Death receptor;Apoptosis2|2427|0更新时间：2025-12-31
- 摘要：Background and purpose: Gene fusions have been identified as recurrent oncogenic events in lung adenocarcinoma. Our purpose are to study the histologic features of anaplastic lymphoma kinase (ALK), c-ros oncogene 1 receptor tyrosine kinase (ROS1) and RET proto-oncogene fusion-positive lung adenocarcinomas and to evaluate the correlation between psammoma bodies and fusion-positive lung adenocarcinomas. Methods: In this study, we performed a comprehensive histologic analysis of 44 fusion-positive (including 15 RET, 20 ALK and 9 ROS1) lung adenocarcinomas and 111 fusion-negative [including 20 epidermal growth factor receptor (EGFR), 20 Kirsten rat sarcoma viral oncogene (K-ras), 71 pan-negative] lung adenocarcinomas. Results: ALK, RET and ROS1 fusionpositive lung adenocarcinomas were more prevalent in solid or acinar predominant adenocarcinoma. Multivariate analysis showed that tumors harboring a fusion gene had significantly higher prevalence of the presence of signet ring cells (P=0.000), micropapillary component (P=0.044), mucinous cribriform pattern (P=0.000) and extracellular mucin (P=0.010). The incidence of psammoma bodies was higher in the lung adenocarcinomas with a gene fusion than in tumors without gene fusions (P=0.000). Psammoma bodies were more likely to be found in tumors with any micropapillary component and/or mucinous cribriform pattern than in tumors lacking a micropapillary component and/or mucinous cribriform pattern (P=0.000). Conclusion: Our data showed that the presence of psammoma bodies, micropapillary component, mucinous cribriform pattern, extracellular mucin or signet ring cells may be either sensitive or specific to predict tumors harboring a fusion gene. These distinct morphologic features may be helpful in selecting cases for further accurate molecular testing.关键词：Fusion gene;Lung adenocarcinomas;Psammoma bodies2|1746|0更新时间：2025-12-31
- 摘要：Background and purpose: Alpha fetoprotein (AFP)-producing gastric cancer (AFPGC) is considered to be a special type of gastric cancer. Currently, the effect on AFPGC is not as good as the common AFP-negative gastric cancer. Therefore, it is very important to explore clinicopathological features of AFPGC cancer, to improve diagnosis and individualized treatment. This study is to investigate the expressions of hepatocyte growth factor receptor c (c-Met), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2 (HER-2) in the AFPGC. Methods: A total number of 44 cases of AFPGC (serum AFP≥10 μg/L) tissues were selected as a test group. There were 30 cases of serum AFP≥200 μg/L. This study collected 30 cases of gastric cancer with normal AFP and 30 cases of hepatocellular carcinoma with increased AFP (serum AFP≥200 μg/L) as 2 control groups. The cases of the 3 groups had the same clinical stage basically. The expressions of c-Met, VEGF, EGFR and HER-2, CD34 were detected by immunohistochemistry (EnVision staining method). Tumor microvessel density (MVD) was calculated by marking CD34, and the results were analyzed. The clinicopathologic parameters were recorded accurately: gender, age, highest level of serum AFP, tumor differentiation, tumor stage, tumor location, lymph node metastasis, liver metastasis, Lauren classification, etc. These patients were followed up regularly. The clinical pathological features of AFPGC patients were investigated. Results: AFPGC clinical characteristics showed that, among 44 cases of AFPGC group, 86.36% (38/44) had lymph node metastasis, 54.55% (24/44) had hepatic metastasis, intestinal type was 36.36% (16/44), diffuse type was 56.82% (25/44), mixed type was 6.82% (3/44). The positive expression rates of c-Met protein in AFPGC, gastric cancer with normal AFP, hepatocellular carcinoma with increased AFP were 73.33% (22/30), 70.00% (21/30) and 53.33% (16/30), respectively. The positive expression rates of VEGF protein were 76.67% (23/30), 56.67% (17/30) and 66.67% (20/30), respectively. The positive expression rates of EGFR protein were 53.33% (16/30), 40.00% (12/30) and 73.33% (22/30), respectively. The “++ and +++” expression rates of HER-2 in AFPGC, gastric cancer with normal AFP and hepatocellular carcinoma with increased AFP were 38.64% (17/44), 23.33% (7/30) and 26.67% (7/30), respectively. The MVD values in AFPGC, gastric cancer with normal AFP and hepatocellular carcinoma with increased AFP were 23.03±10.24, 21.92±11.45 and 19.43±7.83, respectively. Compared with gastric cancer with normal AFP, expression of VEGF protein was significantly higher in the AFPGC (P<0.05). Compared with hepatocellular carcinoma with increased AFP, the expression of c-Met protein was significantly higher in AFPGC (P<0.05). Conclusion: AFPGC is prone to lymph node metastasis and hepatic metastasis. The major part is the diffuse type in Lauren classification. The positive expression rates of VEGF protein in AFPGC were significantly higher than the gastric cancer with normal AFP. The positive expression rates of c-Met protein in AFPGC were significantly higher than the hepatocellular carcinoma with AFP increased.关键词：Gastric carcinoma;Alpha fetoprotein;Vascular endothelial growth factor;c-Met2|3391|0更新时间：2025-12-31
- 摘要：Background and purpose: Osteosarcoma is the most common primary malignant bone tumor in the children and young adults. Until now, there is no prognostic indicator with high specificity and high sensitivity. This study investigated the relationship between miR-92a level in palsma and clinical pathological characteristics, as well as the prognosis in osteosarcoma. Methods: The plasma from 30 healthy volunteers who underwent physical examination and from 45 cases of osteosarcoma before and after operation were collected. The miR-92a level was detected by realtime fluorescent quantitative polymerase chain reaction (RTFQ-PCR) method. According to the relative expression ≥5 of miR-92a, the osteosarcoma patients were divided into 2 groups: miR-92a high expression group and low expression group. Results: The miR-92a level in palsma from osteosarcoma is significantly higer than that from healthy volunteers. Before surgery, the miR-92a level was markedly higher than after surgery. There was no correlation between the miR-92a level and the gender, age, tumor location, tumor size and histological type. However, significant correlation was found with Enneking grade, tumor cell necrosis rate and lung metastasis. Compared with the low-expression group, high miR-92a expression was associated with a significantly shorter overall survival using Kaplan-Meier analysis (P=0.035). Conclusion: The results suggest a significant relationship between miR-92a and survival ratio. miR-92a expression level may be a useful prognostic indicator in osteosarcoma.关键词：Osteosarcoma;miR-92a;Real-time fluorescent quantitative polymerase chain reaction2|1726|0更新时间：2025-12-31
- 摘要：Background and purpose: Visceral pleural invasion (VPI) and vessel invasion (VI) are poor prognostic factors in patients with non-small cell lung cancer (NSCLC). The primary initial recurrence site may be local recurrence in VPI and distant metastasis in VI. The purpose of this study was to validate the prognostic impact and effect of the initial recurrence site of VPI and VI on survival outcomes for NSCLC. Methods: Two hundred and ninety patients who were diagnosed as having NSCLC and underwent lobectomy between Jan. 2007 and Dec. 2013 were retrospectively analyzed. VPI was identified in 51 patients as VPI group, the other 239 patients without VPI as non-VPI group. VI was identified in 29 patients as VI group, the other 261 patients without VI as non-VI group. Clinical characteristics, overall survival (OS), disease-free survival (DFS) were compared. Results: There were statistically significant differences between VPI group and non-VPI group in tumor size, lymph node metastasis, TNM stage and initial recurrence site (P<0.05). Furthermore, there were statistically significant differences between VI group and non-VI group in lymph node metastasis and TNM stage (P<0.05). The 1-, 3- and 5-year OS rates in VPI group (88.2%, 56.7% and 52.7%) were lower than those in non-VPI group (95.8%, 83.7% and 74.0%, P<0.001). The 1-, 3- and 5-year OS rates in VI group (79.3%, 56.8% and 48.7%) were lower than those in non-VI group (96.1%, 81.3% and 72.3%, P=0.001). Cox regression showed TNM stage was a significant prognostic factor for DFS, whereas lymph node metastasis and VPI were significant prognostic factors in patients with NSCLC. Conclusion: The primary initial recurrence site in VPI patients is local recurrence. Patients with VPI or VI may need more postoperative therapy because of their poor prognosis.关键词：Non-small cell lung cancer;Visceral pleural invasion;Vessel invasion;Initial recurrence site2|3903|0更新时间：2025-12-31
- 摘要：Background and purpose: Perioperative hypothermia will affect the prognosis of cancer patients. Amino acid infusion can increase the core temperature by endogenous thermogenesis. And the forced-air warming system has gained high acceptance as a measure for rewarming. This study aimed to find out whether amino acid infusion was effective to treat postoperative hypothermia and how well the treatment effect was when compared with the forced-air warming system. Methods: Fifty-seven ASA Ⅰ or Ⅱ patients aged 18-60 years undergoing elective esophageal or gastric cancer operation under epidural-general anesthesia and whose core temperature were below 36 ℃. When admitted to the recovery room were randomly divided into 3 groups (n=19): Group Ⅰ received intravenous infusion of mixed amino acid at a rate of 2 mL·kg-1·h-1 (A); Group Ⅱ received a forced-air system (B); group Ⅲ received no therapy (C). Rectal temperature and thermal comfort were recorded per 5 min during the first 1 h and oral temperature and thermal comfort were recorded at the 2, 6 and 24 h. ABG was recorded when patients were admitted to the recovery room and at the first hour. Results: At the first hour, the rectal temperature and thermal comfort of groups A and B were higher when compared with group C (P<0.05), and there was no difference between groups A and B (P>0.05). At the second and sixth hour, the temperature and thermal comfort of group A were higher when compared with group B and C (P<0.05), and there was no difference between groups B and C (P>0.05). At the 24th hour, there were no statistically significant differences in the temperature and thermal comfort among the three groups (P>0.05). Conclusion: The rewarming effect of infusion of mixed amino acid is better than that of the forced-air warming system. It is the more effective and convenient method to rewarm the postoperative hypothermia.关键词：Mixed amino acid;Forced-air warming system;Postoperative hypothermia;Tumor metastasis2|1770|0更新时间：2025-12-31
- 摘要：Background and purpose: The incidence of lung cancer with brain metastasis tends to go up. The technique of intensity-modulated radiotherapy (IMRT) with simultaneous integrated boost (SIB) for tumor bed is a new treatment choice for patients with brain metastasis. This study aimed to investigate the feasibility of IMRT combined with SIB for tumor bed in the treatment of brain metastases of lung cancer by exploring its short-term clinical effect and treatment-related toxicities. Methods: Thirty-two patients who were diagnosed as having lung cancer with 1-4 brain metastases were prospectively collected. All the brain metastases were treated with whole brain 5 fields IMRT (40 Gy/20 f) combined with SIB (50 Gy/20 f). The dosage distribution within the target volume and dose-volume histogram were evaluated. Meanwhile, the overall response rate of radiotherapy, the local tumor control rate, 1-year survival rate, treatment-related acute and late toxicities were also calculated. Results: All patients were treated with IMRT successfully. The rate of Grade 2 vomitting, nausea, epilepsy was 9.4%, 15.6%, and 12.5%, respectively. Two cases had Grade 2 cognitive impairment, 4 cases had Grade 2 memory injury, and 2 cases had Grade 3 memory injury. There were no any Grade 4 events of acute and late toxicities. The overall response rate of radiotherapy was 71.9%. The local tumor control rate was 96.9%, and 1-year survival rate was 44%. Conclusion: It is feasible to use 5 fields IMRT (40 Gy/20 f) combined with SIB (boosting 10 Gy, i.e. total dose 50 Gy/20 f) for the treatment of brain metastases of lung cancer.关键词：Intensity-modulated radiotherapy;Lung cancer;Brain metastasis;Simultaneous integrated boost2|2176|0更新时间：2025-12-31
- 摘要：Background and purpose: Fallopian tube carcinosarcoma is a rare malignant tumor that is prone to metastasis and recurrence. Patients with the malignancy often have poor prognosis. This paper retrospectively analyzed the clinical manifestation, pathology, treatment and prognosis of fallopian tube carcinosarcoma. Methods: The clinical manifestations, pathological features, treatment and survival time of 7 cases of fallopian tube carcinosarcoma were retrospectively analyzed. Results: The most common symptoms of 7 cases of fallopian tube carcinosarcoma were irregular vaginal bleeding (4/7, 57.1%). Among 7 cases, one case was stage Ⅰa, 1 stage Ⅱa, 1 stage Ⅱb, 1 stage Ⅲb, and 3 stage Ⅲc. Four cases (57.1%) were heterologous and found to have cartilage components whereas 3 cases (42.9%) were homologous. Seven cases were performed with tumor volume reduction surgery, and 6 cases with chemotherapy, while no one received radiotherapy. Five patients survived and 2 were dead. The survival time of 3 patients was more than 5 years. The longest survival time to the end of the follow-up was 114 months. Conclusion: The pathological examination is the diagnostic means of fallopian tube carcinosarcoma. The prognosis of the patients with the pathological characteristics of the homologous is better than that of the heterologous. Satisfactory reduction of tumor, postoperative chemotherapy with adequate TP regimen, combined with systemic chemotherapy and intraperitoneal chemotherapy may be an effective treatment for fallopian tube carcinosarcoma.关键词：Fallopian tube carcinosarcoma;Clinical manifestation;Pathology;Treatment;Literature review2|2960|0更新时间：2025-12-31
- 摘要：The cancer stem cell theory suggests that cancer develops from a subset of tumor cells that possess characteristics of stem cells. Breast cancer stem cells comprise a sub-population, which possesses the capacity of selfrenewal and the potential for differentiation and high tumorigenicity. Evidence from both in vitro and in vivo studies demonstrates breast cancer stem cells are responsible for tumor relapse, invasion and metastasis, chemo- and radioresistance and epithelial-mesenchymal transition (EMT). Herein, this review highlighted the recent advances in breast cancer stem cells.关键词：Breast cancer stem cells;Stem cell markers;Molecular isoforms;Relapse and metastasis2|3174|0更新时间：2025-12-31
- 摘要：Long non-coding RNA (lncRNA) is a class of RNA molecules, transcripted by RNA polymerase Ⅱ, which consists of more than 200 nucleotides and protein-coding function. Many studies have indicated that lncRNA plays an important role in epigenetics, transcription and post-translational processing. The abnormal expression of lncRNA significantly correlates with occurence, development, and metastasis of renal cell carcinoma (RCC) and prognosis of the patients with RCC. This paper summarizes the advances in the research on lncRNA in RCC to reveal the mechanisms of the disease at the molecular level, in order to provide new methods of prevention, diagnosis, treatment and prognostic assessment of RCC.关键词：Long non-coding RNA;Renal cell carcinoma;Targeted therapy2|2120|0更新时间：2025-12-31
- 关键词：Breast cancer;Ovarian function suppression;Endocrine therapy2|3465|0更新时间：2025-12-31
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