干扰LASP1基因表达对胃癌细胞BGC823增殖、迁移及侵袭能力的影响

doi:10.19401/j.cnki.1007-3639.2017.11.002

中国癌症杂志 ›› 2017, Vol. 27 ›› Issue (11): 847-852.doi: 10.19401/j.cnki.1007-3639.2017.11.002

干扰LASP1基因表达对胃癌细胞BGC823增殖、迁移及侵袭能力的影响

刘玉珍，张梦雪，吕世军，郑洁

潍坊医学院临床病理系，神经疾病与再生修复实验室，山东潍坊 261053

出版日期:2017-11-30 发布日期:2017-12-12
通信作者: 郑洁 E-mail：zj1978824@163.com
基金资助:
国家自然科学基金(81472365)；山东省自然科学基金(ZR2015HL091，ZR2014HM086，ZR2016HL20)；山东省医药卫生科技发展计划(2013WS0279，2014WS0182)；山东省高等学校科技计划(J15LK11)。

Effect of silencing LASP1 on proliferation, migration and invasion of human gastric cancer cell line BGC823

LIU Yuzhen, ZHANG Mengxue, LÜ Shijun, ZHENG Jie

Department of Clinical Pathology, Key Lab of Neurological Disease and Regeneration＆Repair, Weifang Medical University, Weifang 261053, Shandong Province, China

Published:2017-11-30 Online:2017-12-12
Contact: ZHENG Jie E-mail: zj1978824@163.com

摘要/Abstract

摘要： 背景与目的：LASP1是一种新的肌动蛋白结合蛋白，参与细胞骨架的重组调控及细胞迁移，在多种恶性肿瘤中高表达，并与肿瘤的增殖、侵袭和转移密切相关。但目前LASP1在胃癌发生、发展中的作用少见报道。该研究旨在探讨LASP1在胃癌增殖和侵袭中的作用及分子机制。方法：利用实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction，RTFQ-PCR)和蛋白［质］印迹法(Western blot)检测LASP1在不同胃癌细胞系中的表达，应用RNA干扰技术抑制BGC823细胞中LASP1的表达，采用RTFQ-PCR和Western blot检测转染后LASP1的表达，应用体外增殖、迁移和侵袭实验检测转染后细胞的增殖、迁移和侵袭能力，通过F-actin聚合实验检测细胞的F-actin的聚合能力，采用Western blot检测转染前后BGC823细胞中Akt的磷酸化情况。结果：LASP1在胃癌BGC823细胞中高表达。RNA干扰技术沉默LASP1基因后，干扰组与对照组相比，LASP1的mRNA和蛋白表达水平均明显下降。细胞增殖实验显示，干扰组细胞的增殖率低于对照组(P<0.05)；Transwell迁移和侵袭实验发现，与对照组相比，干扰组细胞的迁移和侵袭能力均明显降低(P<0.05)；在干扰组细胞内的F-actin聚合量比对照组减少(P<0.05)；在干扰组细胞内Akt磷酸化受到抑制。结论：LASP1的表达降低可以抑制胃癌BGC823细胞的体外增殖、迁移和侵袭能力，LASP1通过调节F-actin的聚合和Akt磷酸化从而影响胃癌细胞的侵袭、转移。

关键词: LASP1, 人胃癌BGC823细胞, 增殖, 迁移和侵袭

Abstract: Background and purpose: LASP1 is a novel actin-binding protein and involved in the process of cytoskeleton reorganization and cell migration. Expression of LASP1 is significantly up-regulated in several malignant tumors and is closely related to the tumor proliferation, invasion and metastasis. However, the specific mechanism of LASP1 is not clear in gastric carcinogenesis. This study aimed to detect the effect and mechanism of LASP1 in the proliferation and invasion of gastric cancer cells. Methods: Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) and Western blot were employed to determine the expression of LASP1 in gastric cancer cell lines, as well as human normal gastric epithelial cell line GES-1. RNA interference (RNAi) was used to silence the expression of LASP1 in gastric cancer cell BGC823. RTFQ-PCR and Western blot were used to analyze the expression of LASP1 after siRNA transfection. In vitro proliferation, migration and invasion assay were performed to detect the effect of LASP1 on BGC823 cells. F-actin polymerization assay was used to detect F-actin recognition ability in cells. Western blot was used to analyze phosphorylation of Akt. Results: Overexpression of LASP1 was detected in human gastric cancer BGC823 cells. Down-regulated expression of LASP1 at both mRNA and protein level was detected after siRNA transfection. Cell proliferation assay showed that the growth rate was significantly reduced in RNAi group (P<0.05). In transwell assay, the cell migration and invasion were significantly decreased (P<0.05). F-actin content was reduced compared with the control group (P<0.05). The phosphorylation of Akt was significantly reduced after siRNA transfection. Conclusion: LASP1 was highly expressed in gastric cancer cells. Down-regulation of LASP1 suppressed the capacities of proliferation, migration and invasion of human gastric cancer BGC823 cells in vitro. LASP1 can induce migration and invasion of gastric cancer through regulation of F-actin polymerization and Akt phosphorylation.

Key words: LASP1, Human gastric cancer BGC823 cells, Proliferation, Migration and invasion

刘玉珍,张梦雪,吕世军,等. 干扰LASP1基因表达对胃癌细胞BGC823增殖、迁移及侵袭能力的影响[J]. 中国癌症杂志, 2017, 27(11): 847-852.

LIU Yuzhen, ZHANG Mengxue, Lü Shijun, et al. Effect of silencing LASP1 on proliferation, migration and invasion of human gastric cancer cell line BGC823[J]. China Oncology, 2017, 27(11): 847-852.

[1]	杨士鹏, 刘颖, 王馨悦, 杨洋, 全吉淑, 林贞花 . PKLR在胰腺癌中的表达水平及临床病理学意义[J]. 中国癌症杂志, 2021, 31(8): 704-713.
[2]	李海洲, 张艳炜, 许英杰, 杨　门, 张　磊, 韩京军 . miR-933调控KLF6基因影响非小细胞肺癌的作用研究[J]. 中国癌症杂志, 2021, 31(7): 581-588.
[3]	曹达龙, 朱文恺, 施国海, 张海梁, 王子良, 叶定伟. PRUNE2点突变影响前列腺癌DU145细胞增殖、凋亡、侵袭和迁移[J]. 中国癌症杂志, 2021, 31(6): 441-446.
[4]	胡雅琼, 白　俊, 陈　琳, 陈新璐, 张丽萍, 周丹丹, 王　玉, 尹崇高, 李洪利, 刘雨清 . miR-625-5p通过靶向调控PRKACA促进肺腺癌细胞的增殖和侵袭[J]. 中国癌症杂志, 2021, 31(6): 447-454.
[5]	陈　杰, 王　嫣, 张维敏, 赵　笛, 张凌瑗, 詹启敏. 缺氧诱导食管鳞癌细胞中白细胞介素-7表达促进肿瘤进展[J]. 中国癌症杂志, 2021, 31(5): 361-367.
[6]	王春建 , 聂　刚 , 毛　艳 . TRIP6在乳腺癌中的作用及其机制研究[J]. 中国癌症杂志, 2021, 31(3): 161-169.
[7]	贺国洋, 陈庆庆, 邓美静, 王高翔, 王贝玺, 王永霞, 李　巍, 千新来, 朱会芳, . DIAPH3对胃癌细胞增殖、迁移和侵袭的影响及分子机制[J]. 中国癌症杂志, 2021, 31(12): 1174-1184.
[8]	张进忠, 李悦淇, 石　科, 杨　亮, 郭　丹 . PLK4基因在人食管鳞癌组织中的表达及对癌细胞增殖、侵袭迁移影响的研究[J]. 中国癌症杂志, 2021, 31(12): 1185-1193.
[9]	吴　冉, 王桂珍, 程　昕, 周光飚 . 铜离子对肺癌细胞增殖的影响及相关基因的临床意义[J]. 中国癌症杂志, 2021, 31(11): 1072-1080.
[10]	王　佳, 艾中中, 周　菁 . 下调SLC6A3对肾透明细胞癌细胞SNU-349增殖、迁移的影响[J]. 中国癌症杂志, 2021, 31(1): 20-26.
[11]	陈劭赓, 何荣琦, 张万飞, 林贤钻, 陈河山, 许荣誉. miR-122-5p通过靶向CREB1抑制食管癌细胞及移植瘤的生长[J]. 中国癌症杂志, 2021, 31(1): 35-44.
[12]	程　玉, 郝美玲, 薛　晶, 齐洁敏 . 节律因子BMAL1通过SHH信号转导通路影响胃癌MGC-803细胞的增殖[J]. 中国癌症杂志, 2020, 30(9): 661-666.
[13]	田连芳, 李丽玮, 张小冲, 郑丽春, 左江华, 孙春秀, 刘登湘. miR-26b-3p对乳腺癌细胞生物学行为的影响及作用机制研究[J]. 中国癌症杂志, 2020, 30(9): 667-673.
[14]	祝开忠, 王挺锐, 陈焕雄, 陈　涛, 钟贞浩. 骨肉瘤中O-GlcNAc糖基转移酶的表达及其对增殖和顺铂敏感性的影响[J]. 中国癌症杂志, 2020, 30(9): 682-688.
[15]	文　静, 黄　洁, 李云云, 张中卒, 周　勤 . 肿瘤相关巨噬细胞相关性miR-99a对子宫内膜癌细胞生长和侵袭的调控作用[J]. 中国癌症杂志, 2020, 30(8): 561-569.

干扰LASP1基因表达对胃癌细胞BGC823增殖、迁移及侵袭能力的影响

Effect of silencing LASP1 on proliferation, migration and invasion of human gastric cancer cell line BGC823

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价