环状RNA hsa_circ_0012779在鼻咽癌中的表达及其对细胞生物学行为影响的机制研究

doi:10.19401/j.cnki.1007-3639.2023.05.004

中国癌症杂志 ›› 2023, Vol. 33 ›› Issue (5): 445-451.doi: 10.19401/j.cnki.1007-3639.2023.05.004

环状RNA hsa_circ_0012779在鼻咽癌中的表达及其对细胞生物学行为影响的机制研究

章平川¹^,²(), 杜鸣宇¹, 姚成云¹, 何侠¹^,², 尹丽¹^,²()

1.南京医科大学附属肿瘤医院，江苏省肿瘤医院，江苏省肿瘤防治研究所放疗科，江苏南京 210009
2.南京医科大学第四临床医学院，江苏南京 210000

收稿日期:2022-11-04 修回日期:2023-03-04 出版日期:2023-05-30 发布日期:2023-06-16
通信作者: 尹丽（ORCID: 0009-0004-2669-4087），博士，副教授。
作者简介:章平川（ORCID: 0009-0005-3185-5612），硕士。
基金资助:
江苏省卫生健康委员会面上项目(M2022108);南京医科大学肿瘤个体化医学省部共建协同创新中心基金(JZ21449020210616)

Mechanism of circular RNA hsa_circ_0012779 expression in nasopharyngeal carcinoma and its influence on cell biological behavior

ZHANG Pingchuan¹^,²(), DU Mingyu¹, YAO Chengyun¹, HE Xia¹^,², YIN Li¹^,²()

1. Department of Radiotherapy, The Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing 210009, Jiangsu Province, China
2. The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing 210000, Jiangsu Province, China

Received:2022-11-04 Revised:2023-03-04 Published:2023-05-30 Online:2023-06-16
Contact: YIN Li

摘要/Abstract

摘要：

背景与目的：环状RNA（circular RNA，circRNA）在多种肿瘤的发展过程中发挥重要的调节作用。然而，circRNA在鼻咽癌中的异常表达和生物学功能仍不清楚。本研究旨在探讨hsa_circ_0012779对鼻咽癌细胞生物学行为的影响及其分子机制。方法：通过实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction，RTFQ-PCR）检测正常人鼻咽上皮细胞NP69及鼻咽癌细胞系CNE2、5-8F、HNE1、SUNE1中hsa_circ_0012779的表达。采用细胞计数试剂盒-8（cell counting kit-8，CCK-8）实验和transwell侵袭实验检测hsa_circ_0012779对鼻咽癌细胞增殖和侵袭能力的影响。采用蛋白质印迹法（Western blot）检测hsa_circ_0012779对ELAV样蛋白1（ELAV like protein 1，ELAVL1）表达的影响，通过RNA下拉实验验证hsa_circ_0012779与ELAVL1的直接结合。结果：hsa_circ_0012779在鼻咽癌组织和细胞中高表达，敲减hsa_circ_0012779能抑制鼻咽癌细胞增殖和侵袭，hsa_circ_0012779与RNA结合蛋白ELAVL1结合促进其表达并且共定位于细胞质中。同时，敲减hsa_circ_0012779对鼻咽癌细胞的影响能被ELAVL1过表达逆转。结论：hsa_circ_0012779促进ELAVL1的表达，进而促进鼻咽癌细胞增殖和侵袭，影响鼻咽癌的发生、发展。

关键词: 环状RNA, 鼻咽癌, hsa_circ_0012779, ELAV样蛋白1, 增殖, 侵袭

Abstract:

Background and purpose: Circular RNA (circRNA) plays an important regulatory role in the development of a variety of tumors. However, the abnormal expression and biological function of circRNA in nasopharyngeal carcinoma remain unclear. This study aimed to explore the effect of hsa_circ_0012779 on the biological behavior of nasopharyngeal carcinoma cells and its molecular mechanism. Methods: The expression of hsa_circ_0012779 in human immortalized nasopharyngeal epithelial cell line NP69 and nasopharyngeal carcinoma cell lines CNE2, 5-8F, HNE1 and SUNE1 was detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Cell counting kit-8 (CCK-8) assay and transwell invasion assay were used to detect the effect of hsa_circ_0012779 on the proliferation and invasion ability of nasopharyngeal carcinoma cells. The protein level of ELAV like protein 1 (ELAVL1) in NPC cells with hsa_circ_0012779 knockdown was detected by Western blot. The binding of hsa_circ_0012779 and ELAVL1 was verified by RNA pull-down assay. Results: hsa_circ_0012779 was highly expressed in nasopharyngeal carcinoma tissues and cells. Knockdown hsa_circ_0012779 could inhibit the proliferation and invasion of nasopharyngeal carcinoma cells. hsa_circ_0012779 bound to RNA-binding protein ELAVL1 to promote its expression and colocalization in cytoplasm. In the meanwhile, the effect of knockdown hsa_circ_0012779 on nasopharyngeal carcinoma cells could be reversed by the overexpression of ELAVL1. Conclusion: hsa_circ_0012779 promote the expression of ELAVL1 and thus promote the proliferation and invasion of nasopharyngeal carcinoma, and influence the occurrence and development of nasopharyngeal carcinoma.

Key words: Circular RNA, Nasopharyngeal carcinoma, hsa_circ_0012779, ELAV like protein 1, Proliferation, Invasion

中图分类号:

R739.6

章平川, 杜鸣宇, 姚成云, 何侠, 尹丽. 环状RNA hsa_circ_0012779在鼻咽癌中的表达及其对细胞生物学行为影响的机制研究[J]. 中国癌症杂志, 2023, 33(5): 445-451.

ZHANG Pingchuan, DU Mingyu, YAO Chengyun, HE Xia, YIN Li. Mechanism of circular RNA hsa_circ_0012779 expression in nasopharyngeal carcinoma and its influence on cell biological behavior[J]. China Oncology, 2023, 33(5): 445-451.

图/表 4

参考文献 13

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环状RNA hsa_circ_0012779在鼻咽癌中的表达及其对细胞生物学行为影响的机制研究

Mechanism of circular RNA hsa_circ_0012779 expression in nasopharyngeal carcinoma and its influence on cell biological behavior

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