环状RNA hsa_circ_0012779在鼻咽癌中的表达及其对细胞生物学行为影响的机制研究

doi:10.19401/j.cnki.1007-3639.2023.05.004

摘要/Abstract

摘要：

背景与目的：环状RNA（circular RNA，circRNA）在多种肿瘤的发展过程中发挥重要的调节作用。然而，circRNA在鼻咽癌中的异常表达和生物学功能仍不清楚。本研究旨在探讨hsa_circ_0012779对鼻咽癌细胞生物学行为的影响及其分子机制。方法：通过实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction，RTFQ-PCR）检测正常人鼻咽上皮细胞NP69及鼻咽癌细胞系CNE2、5-8F、HNE1、SUNE1中hsa_circ_0012779的表达。采用细胞计数试剂盒-8（cell counting kit-8，CCK-8）实验和transwell侵袭实验检测hsa_circ_0012779对鼻咽癌细胞增殖和侵袭能力的影响。采用蛋白质印迹法（Western blot）检测hsa_circ_0012779对ELAV样蛋白1（ELAV like protein 1，ELAVL1）表达的影响，通过RNA下拉实验验证hsa_circ_0012779与ELAVL1的直接结合。结果：hsa_circ_0012779在鼻咽癌组织和细胞中高表达，敲减hsa_circ_0012779能抑制鼻咽癌细胞增殖和侵袭，hsa_circ_0012779与RNA结合蛋白ELAVL1结合促进其表达并且共定位于细胞质中。同时，敲减hsa_circ_0012779对鼻咽癌细胞的影响能被ELAVL1过表达逆转。结论：hsa_circ_0012779促进ELAVL1的表达，进而促进鼻咽癌细胞增殖和侵袭，影响鼻咽癌的发生、发展。

关键词: 环状RNA, 鼻咽癌, hsa_circ_0012779, ELAV样蛋白1, 增殖, 侵袭

Abstract:

Background and purpose: Circular RNA (circRNA) plays an important regulatory role in the development of a variety of tumors. However, the abnormal expression and biological function of circRNA in nasopharyngeal carcinoma remain unclear. This study aimed to explore the effect of hsa_circ_0012779 on the biological behavior of nasopharyngeal carcinoma cells and its molecular mechanism. Methods: The expression of hsa_circ_0012779 in human immortalized nasopharyngeal epithelial cell line NP69 and nasopharyngeal carcinoma cell lines CNE2, 5-8F, HNE1 and SUNE1 was detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Cell counting kit-8 (CCK-8) assay and transwell invasion assay were used to detect the effect of hsa_circ_0012779 on the proliferation and invasion ability of nasopharyngeal carcinoma cells. The protein level of ELAV like protein 1 (ELAVL1) in NPC cells with hsa_circ_0012779 knockdown was detected by Western blot. The binding of hsa_circ_0012779 and ELAVL1 was verified by RNA pull-down assay. Results: hsa_circ_0012779 was highly expressed in nasopharyngeal carcinoma tissues and cells. Knockdown hsa_circ_0012779 could inhibit the proliferation and invasion of nasopharyngeal carcinoma cells. hsa_circ_0012779 bound to RNA-binding protein ELAVL1 to promote its expression and colocalization in cytoplasm. In the meanwhile, the effect of knockdown hsa_circ_0012779 on nasopharyngeal carcinoma cells could be reversed by the overexpression of ELAVL1. Conclusion: hsa_circ_0012779 promote the expression of ELAVL1 and thus promote the proliferation and invasion of nasopharyngeal carcinoma, and influence the occurrence and development of nasopharyngeal carcinoma.

Key words: Circular RNA, Nasopharyngeal carcinoma, hsa_circ_0012779, ELAV like protein 1, Proliferation, Invasion

中图分类号:

R739.6

章平川, 杜鸣宇, 姚成云, 何侠, 尹丽. 环状RNA hsa_circ_0012779在鼻咽癌中的表达及其对细胞生物学行为影响的机制研究[J]. 中国癌症杂志, 2023, 33(5): 445-451.

ZHANG Pingchuan, DU Mingyu, YAO Chengyun, HE Xia, YIN Li. Mechanism of circular RNA hsa_circ_0012779 expression in nasopharyngeal carcinoma and its influence on cell biological behavior[J]. China Oncology, 2023, 33(5): 445-451.

图/表 4

参考文献 13

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