关键词: FAM83A, 肺腺癌, 预后, 增殖, 侵袭, 转移

Abstract: Background and purpose: A growing number of studies have shown that family with sequence similarity 83 member A (FAM83A) is correlated with the invasion and metastasis of tumor, however, the role of FAM83A in lung adenocarcinoma is unclear. This study aimed to explore FAM83A expression in lung adenocarcinoma tissues, and its relationship with prognosis of patients with lung adenocarcinoma and the abilities of lung adenocarcinoma cancer cell proliferation, invasion and metastasis. Methods: The Human Protein Atlas (HPA) and Gene Expression Profiling Interactive Analysis (GEPIA) databases were used to analyze and to select the genes related to the prognosis of lung adenocarcinoma. The UALCAN and GEPIA databases were used to study the correlation between FAM83A expression level and survival time of patients with lung adenocarcinoma. The UALCAN database was used to analyze the expression of FAM83A in lung adenocarcinoma tissues and its correlation among the gender, lymph node stage and metastasis in lung adenocarcinoma patients. The expression and transfection efficiency of FAM83A protein in human normal lung epithelial cells BEAS-2B and lung adenocarcinoma cells A549 were detected by Western blot. The effect of FAM83A knockdown after transfection on the proliferation of lung adenocarcinoma cells was detected by cell counting kit-8 (CCK-8) cell proliferation assay. Transwell invasion assay examined the effect of FAM83A on the invasion ability of lung adenocarcinoma cells. Results: HPA, GEPIA and UALCAN databases were used to analyze the correlation between FAM83A and the prognosis of lung adenocarcinoma, and FAM83A was highly expressed in lung adenocarcinoma tissues. The survival rate of lung adenocarcinoma patients with high expression of FAM83A was low (P<0.05). The expression of FAM83A was correlated with the clinical stage and lymph node metastasis of lung adenocarcinoma, but not with the patient's gender. Western blot results showed that FAM83A was highly expressed in lung adenocarcinoma cells and transfected successfully with reduced plasmid. The results of CCK-8 cell proliferation assay showed that the proliferation capacity of A549/siFAM83A cells in the knockdown group was significantly reduced compared with A549/Scr cells in the control group (P<0.05). Transwell invasion experiments showed that the invasion capacity of A549 was significantly reduced after FAM83A was subtracted (P<0.05). Conclusion: FAM83A was highly expressed in lung adenocarcinoma tissues and cells and promoted the proliferation, invasion and metastasis of lung adenocarcinoma cells.

Key words:  , FAM83A, Lung adenocarcinoma, Prognosis, Proliferation, Invasion, Metastasis