中国癌症杂志 ›› 2020, Vol. 30 ›› Issue (8): 586-592.doi: 10.19401/j.cnki.1007-3639.2020.08.004

• 论著 • 上一篇    下一篇

FAM83A在肺腺癌组织中高表达且促进肺腺癌细胞的侵袭和转移

王晓栋 1 ,周丹丹 2 ,张丽萍 2 ,郑 荃 2 ,牟青杰 3 ,尹崇高 4 ,李洪利 5   

  1. 1. 潍坊医学院生命科学与技术学院,山东 潍坊 261053 ;
    2. 潍坊医学院病理学教研室,山东 潍坊 261053 ;
    3. 潍坊医学院临床学院,山东 潍坊 261053 ;
    4. 潍坊医学院护理学院,山东 潍坊 261053 ;
    5. 潍坊医学院医学研究实验中心,山东 潍坊 261053
  • 出版日期:2020-08-30 发布日期:2020-09-03
  • 通信作者: 李洪利 E-mail: lihongli@wfmc.edu.cn
  • 基金资助:
    国家自然科学基金(81702932);山东省自然科学基金(ZR2019MH033);山东省高等学校青创人才引育计划:潍坊市科学技术发展计划(高校部分)项目(2018GX077);潍坊市科技局医学类项目(2019YX029);山东省大学生创新创业训练计划项目(S201910438001);潍坊医学院大学生科技创新基金(KX 2019017,KX2019078)。

FAM83A is highly expressed in lung adenocarcinoma tissues and promotes invasion and metastasis of lung adenocarcinoma cells

WANG Xiaodong 1 , ZHOU Dandan 2 , ZHANG Liping 2 , ZHENG Quan 2 , MU Qingjie 3 , YIN Chonggao 4 , LI Hongli 5   

  1. 1. College of Life Science and Technology, Weifang Medical University, Weifang 261053, Shandong Province, China; 2. Department of Pathology, Weifang Medical University, Weifang 261053, Shandong Province, China; 3. College of Clinical Medicine, Weifang Medical University, Weifang 261053, Shandong Province, China; 4. College of Nursing, Weifang Medical University, Weifang 261053, Shandong Province, China; 5. Medicine Research Center, Weifang Medical University, Weifang 261053, Shandong Province, China
  • Online:2020-08-30 Published:2020-09-03
  • Contact: LI Hongli E-mail: lihongli@wfmc.edu.cn

摘要: 背景与目的:越来越多的研究表明,序列相似性为83的家族成员A(family with sequence similarity 83 member A,FAM83A)与肿瘤的侵袭和转移相关,但FAM83A在肺腺癌中的作用尚不清楚。探讨FAM83A在肺腺癌组织中的表达情况,与肺腺癌患者预后的关系以及对肺腺癌细胞增殖、侵袭转移能力的影响。方法:运用人类蛋白质图谱(Human Protein Atlas,HPA)和基因表达谱动态分析(Gene Expression Profiling Interactive Analysis,GEPIA)数据库分析并选取与肺腺癌预后相关的基因;运用UALCAN和GEPIA数据库研究FAM83A的表达量对肺腺癌生存率的影响;采用UALCAN数据库分析FAM83A在肺腺癌组织中的表达及其与肺腺癌患者的性别、淋巴结的分期和转移的关系;采用蛋白质印迹法(Western blot)检测人正常肺上皮细胞BEAS-2B和肺腺癌细胞A549中FAM83A蛋白的表达情况及FAM83A的转染效率;采用细胞计数试剂盒(cell counting kit-8,CCK-8)检测转染后敲减FAM83A对肺腺癌细胞增殖能力的影响;采用transwell侵袭实验检测FAM83A对肺腺癌细胞侵袭能力的影响。结果:采用HPA、GEPIA和UALCAN数据库分析发现高表达的FAM83A与肺腺癌不良预后相关且FAM83A在肺腺癌组织中高表达,差异有统计学意义(P<0.05);FAM83A的表达与肺腺癌的临床分期和淋巴结转移相关;Western blot检测结果显示,FAM83A在肺腺癌细胞中高表达且敲减质粒转染成功;CCK-8细胞增殖实验结果提示FAM83A可促进细胞的增殖能力(P<0.05);Transwell侵袭实验提示FAM83A促进A549的侵袭能力(P<0.05)。结论:FAM83A在肺腺癌组织和细胞中高表达且促进肺腺癌细胞的增殖、侵袭和转移。

关键词: FAM83A, 肺腺癌, 预后, 增殖, 侵袭, 转移

Abstract: Background and purpose: A growing number of studies have shown that family with sequence similarity 83 member A (FAM83A) is correlated with the invasion and metastasis of tumor, however, the role of FAM83A in lung adenocarcinoma is unclear. This study aimed to explore FAM83A expression in lung adenocarcinoma tissues, and its relationship with prognosis of patients with lung adenocarcinoma and the abilities of lung adenocarcinoma cancer cell proliferation, invasion and metastasis. Methods: The Human Protein Atlas (HPA) and Gene Expression Profiling Interactive Analysis (GEPIA) databases were used to analyze and to select the genes related to the prognosis of lung adenocarcinoma. The UALCAN and GEPIA databases were used to study the correlation between FAM83A expression level and survival time of patients with lung adenocarcinoma. The UALCAN database was used to analyze the expression of FAM83A in lung adenocarcinoma tissues and its correlation among the gender, lymph node stage and metastasis in lung adenocarcinoma patients. The expression and transfection efficiency of FAM83A protein in human normal lung epithelial cells BEAS-2B and lung adenocarcinoma cells A549 were detected by Western blot. The effect of FAM83A knockdown after transfection on the proliferation of lung adenocarcinoma cells was detected by cell counting kit-8 (CCK-8) cell proliferation assay. Transwell invasion assay examined the effect of FAM83A on the invasion ability of lung adenocarcinoma cells. Results: HPA, GEPIA and UALCAN databases were used to analyze the correlation between FAM83A and the prognosis of lung adenocarcinoma, and FAM83A was highly expressed in lung adenocarcinoma tissues. The survival rate of lung adenocarcinoma patients with high expression of FAM83A was low (P<0.05). The expression of FAM83A was correlated with the clinical stage and lymph node metastasis of lung adenocarcinoma, but not with the patient's gender. Western blot results showed that FAM83A was highly expressed in lung adenocarcinoma cells and transfected successfully with reduced plasmid. The results of CCK-8 cell proliferation assay showed that the proliferation capacity of A549/siFAM83A cells in the knockdown group was significantly reduced compared with A549/Scr cells in the control group (P<0.05). Transwell invasion experiments showed that the invasion capacity of A549 was significantly reduced after FAM83A was subtracted (P<0.05). Conclusion: FAM83A was highly expressed in lung adenocarcinoma tissues and cells and promoted the proliferation, invasion and metastasis of lung adenocarcinoma cells.

Key words:  , FAM83A, Lung adenocarcinoma, Prognosis, Proliferation, Invasion, Metastasis