中国癌症杂志 ›› 2013, Vol. 23 ›› Issue (9): 703-708.doi: 10.3969/j.issn.1007-3969.2013.09.002

• 论著 • 上一篇    下一篇

微小RNA-187*在结直肠癌组织中表达及其对结肠癌细胞凋亡的影响

刘博,田延锋,赵增仁,樊智彬,张丽静,贺新奇,高利飞   

  1. 河北医科大学第一医院普通外科,河北 石家庄 050031
  • 出版日期:2013-09-25 发布日期:2014-02-20
  • 通信作者: 赵增仁 E-mail:zzr-doctor@163.com
  • 基金资助:
    国家自然科学基金资助项目(No: 81072034);
    河北省自然科学基金资助项目(No: C2011206103);
    河北省科技计划项目(No: 12396105D)

Down-regulation of microRNA-187* expression in colorectal cancer and its roles in promoting cell apoptosis

LIU Bo,TIAN Yan-feng,ZHAO Zeng-ren,FAN Zhi-bin,ZHANG Li-jing,HE Xin-qi,GAO Li-fei   

  1. Department of General Surgery, First Hospital of Hebei Medical University, Shijiazhuang Hebei 050031, China
  • Published:2013-09-25 Online:2014-02-20
  • Contact: ZHAO Zeng-ren E-mail: zzr-doctor@163.com

摘要:

背景与目的:微小RNA(microRNA, miRNA)在细胞分化、细胞周期及凋亡过程中发挥重要作用。miRNA通过扩增、缺失、突变和沉默等机制影响癌症的发生、发展。本研究探讨miR-187*在结直肠癌组织中的表达和临床意义,以及上调miR-187*表达量对结肠癌细胞凋亡的影响。方法:采用实时荧光定量反转录聚合酶链反应(real-time quantitative reverse transcription-PCRreal-time PCR)的方法在40例结直肠癌患者组织标本中检测miR-187*的表达水平,结合病理资料分析其临床意义。HCT116细胞转染miR-187*mimics后,用Annexin- FITC/PI流式细胞术检测miR-187*对凋亡的影响。结果:miR-187*在结直肠癌组织中表达量为0.165(0.1060.428),明显低于正常黏膜组织表达量[0.334(0.2110.712)],差异有统计学意义(P<0.05),且在黏液癌及高龄患者中下调更明显(P<0.05)。将miR-187*mimics转染至HCT116中可提高其表达量,通过流式技术检测发现,与对照组早期凋亡率[(23.010±1.279)%]相比较,实验组早期凋亡率[(26.748±1.098)%]升高,差异有统计学意义(P0.05)结论:miR-187*在结直肠癌组织中低表达,并且与组织学类型及年龄有关;miR-187*表达上调可促进HCT116早期凋亡;miR-187*具有潜在抑癌作用。

关键词: 结直肠肿瘤, 微小RNA, microRNA-187*, 凋亡

Abstract:

Background and purpose: MicroRNAs (miRNAs) play an important role in tumor biological behavior. miRNAs are down-regulated or up-regulated in various cancer types, triggering abnormal cell differentiation, proliferation and apoptosis. This study was designed to investigate the expression and clinical significance of miR-187* in colorectal cancer (CRC), and further to investigate its roles in promoting cell apoptosis. Methods: The expressions of miR-187* in 40 CRC cases were examined by real-time quantitative reverse transcription-PCR (qRT-PCR). The relationship between miR-187* expression and clinical features of CRC was analyzed. HCT116 cells were transfected with a miR-187* mimic and the apoptosis of the transfected cells were examined by flow cytometry (FCM). Results: The expression of miR-187* was down-regulated in CRC tissues 0.165 (0.106, 0.428) compared with those in normal tissues 0.334 (0.211, 0.712) (P<0.05), especially in mucinous carcinoma and older age CRC (P<0.05). Transfection of HCT116 cells with a miR-187* mimic up-regulated the expression of miR-187* and increased cell early apoptosis (P<0.05). Conclusion: The expression level of miR-187* was lower in CRC. miR-187* expression correlates with histological type and age. Transfection of HCT116 cells with a miR-187* mimic accelerates apoptosis of tumor cells, suggesting that miR-187* is a potent tumor suppressor.

Key words: Colorectal cancer, microRNA, microRNA-187*, Apoptosis