蟾蜍灵对宫颈癌细胞的增殖抑制作用及其机制研究

doi:10.3969/j.issn.1007-3969.2015.10.004

中国癌症杂志 ›› 2015, Vol. 25 ›› Issue (10): 780-784.doi: 10.3969/j.issn.1007-3969.2015.10.004

蟾蜍灵对宫颈癌细胞的增殖抑制作用及其机制研究

刘　霏¹，李浩然¹，程　玺¹，李梦娇¹，王子良²，杨　莉³

1. 复旦大学附属肿瘤医院妇科，复旦大学上海医学院肿瘤学系，上海 200032 ；
2. 复旦大学附属肿瘤医院肿瘤研究所，复旦大学上海医学院肿瘤学系，上海 200032 ；
3. 浙江省肿瘤医院妇科，浙江杭州 310002

出版日期:2015-10-30 发布日期:2015-12-17
通信作者: 程　玺　E-mail:cheng_xi1@hotmail.com
基金资助:
国家自然科学基金青年科学基金项目(NSFC1212)；上海市卫生局中医药科研基金(WS-ZY1201)。

Research on the cytostatic effect of bufalin on cervical carcinoma cells and the related mechanism

LIU Fei¹, LI Haoran¹, CHENG Xi¹, LI Mengjiao¹, WANG Ziliang², YANG Li³

1.Department of Gynecological Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Department of Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai 200032, China; 3.Department of Gynecological Oncology, Zhejiang Cancer Hospital, Hangzhou 310002, Zhejiang, China

Published:2015-10-30 Online:2015-12-17
Contact: CHENG Xi E-mail: cheng_xi1@hotmail.com

摘要/Abstract

摘要： 背景与目的：宫颈癌仍然是妇科肿瘤致死病因的第二位，部分原因为化疗耐药。蟾蜍灵是传统中药蟾酥的成分之一，目前在国内广泛应用于肿瘤的治疗中。该研究旨在探讨蟾蜍灵对宫颈癌细胞ME180和C33A的增殖抑制作用及相关机制。方法：采用CCK8(cell counting kit-8)法检测蟾蜍灵对宫颈癌细胞增殖的抑制作用，采用葡萄糖检测分析试剂盒(glucose assay kit)进行细胞内糖代谢检测分析，采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction，qRT-PCR)检测细胞内葡萄糖转运蛋白1(glucose transporter 1，GLUT1)和己糖激酶2(hexokinase 2，HK2)的mRNA的表达水平，采用蛋白［质］印迹法(Western blot)检测原癌基因C-MYC和缺氧诱导因子1α(Hif-1α)表达的变化。结果：CCK8法结果显示，蟾蜍灵能明显抑制宫颈癌细胞ME180和C33A细胞增殖(P=0.027，P=0.018)。糖代谢检测结果显示，蟾蜍灵处理组葡萄糖代谢水平显著降低(P=0.034，P=0.036)。qRT-PCR检测结果显示，蟾蜍灵处理组糖代谢相关指标GLUT1(P=0.019，P=0.016)和HK2(P=0.039，P=0.041)的表达水平明显下调。Western blot检测结果显示，蟾蜍灵处理后宫颈癌细胞内C-MYC和Hif-1α蛋白表达水平显著下调。结论：蟾蜍灵可以通过降低宫颈癌细胞ME180和C33A糖代谢水平，从而抑制细胞增殖。

关键词: 蟾蜍灵, 宫颈癌, 细胞增殖, 葡萄糖代谢

Abstract: Background and purpose: Cervical cancer remains the second leading cause of death in gynecologic malignancies partially because of resistance to chemotherapy. Bufalin, a component of the traditional Chinese medicine Chansu, has been widely used in cancer treatment in China. This study aimed to investigate the effects of bufalin on inhibiting the proliferation of ME180 and C33A and explore its possible mechanism. Methods: The cytostatic effects of bufalin on ME180 and C33A cells were evaluated by CCK8 assay (cell counting kit-8). Glucose levels in ME180 and C33A cells were measured using glucose assay kit. Then the alterations of GLUT1 (glucose transporter 1) and HK2 (hexokinase 2) gene expression were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The expressions of proto-oncogene C-MYC and HIF1α (hypoxia-inducible factor 1α) were determined by Western blot. Results: According to the results of CCK-8, bufalin can significantly inhibit the proliferation of carcinoma cells ME180 and C33A (P=0.027, P=0.018). Test on glycometabolism indicated that glucose uptake in cells treated with bufalin decreased (P=0.034, P=0.036). Results from real-time PCR showed that the expression of glycometabolism related indicators GLUT1 (P=0.019) and HK2 (P=0.016) levels were significantly down-regulated in bufalin treated group. Western blot showed that the expression of C-MYC and HIF1αin cells with bufalin treatment was down-regulated markedly. Conclusion: Bufalin can inhibit the proliferation of the cervical carcinoma cells ME180 and C33A through inhibition of their glucose metabolism.

Key words: Bufalin, Cervical neoplasm, Cellular proliferation, Glucose metabolism

刘　霏,李浩然,程　玺,等. 蟾蜍灵对宫颈癌细胞的增殖抑制作用及其机制研究[J]. 中国癌症杂志, 2015, 25(10): 780-784.

LIU Fei, LI Haoran, CHENG Xi, et al. Research on the cytostatic effect of bufalin on cervical carcinoma cells and the related mechanism[J]. China Oncology, 2015, 25(10): 780-784.

[1]	方艳惠, 马彩娟, 张纯丽, 翟嘉威, 朱巧英, 刘茵, 赵喜娃. miR-496通过SFMBT1抑制子宫颈癌HeLa细胞增殖、迁移和侵袭的相关研究[J]. 中国癌症杂志, 2021, 31(8): 697-703.
[2]	龙行涛, 周琦, 王冬, 陈月梅, 金福军 . 子宫颈癌2018年FIGO新分期ⅢC期患者预后分析[J]. 中国癌症杂志, 2021, 31(8): 725-733.
[3]	李海洲, 张艳炜, 许英杰, 杨　门, 张　磊, 韩京军 . miR-933调控KLF6基因影响非小细胞肺癌的作用研究[J]. 中国癌症杂志, 2021, 31(7): 581-588.
[4]	中国抗癌协会妇科肿瘤专业委员会. 子宫颈癌诊断与治疗指南（2021年版）[J]. 中国癌症杂志, 2021, 31(6): 474-489.
[5]	董诗洁, 胡晓欣, 王　葳, 杨　孟, 岳　磊, 童　彤, 顾雅佳. 基于多序列MRI与多体系影像组学模型预测子宫颈癌淋巴结转移的研究[J]. 中国癌症杂志, 2021, 31(6): 460-467.
[6]	朱　俊, 吴小华. 2020年度妇科恶性肿瘤最新研究进展及展望[J]. 中国癌症杂志, 2021, 31(4): 250-256.
[7]	吴　冉, 王桂珍, 程　昕, 周光飚 . 铜离子对肺癌细胞增殖的影响及相关基因的临床意义[J]. 中国癌症杂志, 2021, 31(11): 1072-1080.
[8]	程　玉, 郝美玲, 薛　晶, 齐洁敏 . 节律因子BMAL1通过SHH信号转导通路影响胃癌MGC-803细胞的增殖[J]. 中国癌症杂志, 2020, 30(9): 661-666.
[9]	文　静, 黄　洁, 李云云, 张中卒, 周　勤 . 肿瘤相关巨噬细胞相关性miR-99a对子宫内膜癌细胞生长和侵袭的调控作用[J]. 中国癌症杂志, 2020, 30(8): 561-569.
[10]	燕飞虎 , 邹　瑞 , 王一尧 , 王芳芳 , 冉浩男 , 王　燕 . PRMT5在胃癌中的表达及其对胃癌细胞增殖、凋亡、迁移能力的影响[J]. 中国癌症杂志, 2020, 30(4): 275-283.
[11]	沈　洁, 莫　淼, 袁　晶, 周昌明, 王泽洲, 张志红, 温　灏, 吴小华, 郑　莹 . 1 313例局部晚期宫颈癌患者的长期生存报告：以医院为基础的真实世界研究[J]. 中国癌症杂志, 2020, 30(3): 192-198.
[12]	陈晓慧, 王佳舟, 胡伟刚, 彭佳元, 翟　鹏. 加速器模型和治疗计划系统对知识库计划模型的影响：一项基于宫颈癌适形调强放射放疗计划的研究[J]. 中国癌症杂志, 2020, 30(10): 821-825.
[13]	王　利,任玉兰,王华英 . 宫颈癌根治术后肺栓塞1例报道[J]. 中国癌症杂志, 2019, 29(8): 599-601.
[14]	刘山，房娟，王黎黎，张映红，杜文杰，朱桃花. 宫颈癌周围神经侵犯与复发高危因素的关联研究[J]. 中国癌症杂志, 2019, 29(6): 439-444.
[15]	张能，苏鹏，陈书练，李晓光，黄翔，罗旭. 前列腺癌中锌指蛋白ZIC2表达及对细胞增殖和凋亡的影响[J]. 中国癌症杂志, 2019, 29(4): 250-256.

蟾蜍灵对宫颈癌细胞的增殖抑制作用及其机制研究

Research on the cytostatic effect of bufalin on cervical carcinoma cells and the related mechanism

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价