关键词: 喉癌, BTG1, 增殖, 凋亡, 细胞周期

Abstract: Background and purpose: B-cell translocation gene 1(BTG1) can inhibit cell proliferation, promote cell apoptosis and regulate cell cycle progression and differentiation in a variety of cell types. This study aimed to explore the influence on cell proliferation, apoptosis and cell cycle and its related mechanism of laryngeal cancer Hep - 2 cell lines through BTG1 overexpression by in vitro experiments. Methods: The BTG1 expression plasmids were constructed and transfected into Hep-2. They were divided into experimental group (transfected BTG1 of Hep-2 cells) and control group (transfected empty plasmid of Hep-2 cells). Western blot method was used to identify BTG1 protein expression levels of cells; proliferation activity of cells was detected by MTT assay; flow cytometry was used to analyze the cell cycle distribution and Annexin Ⅴ-FITC/PI cell apoptosis; Western blot was also used to assay cell cycle regulatory protein and apoptosis-related protein expression. Results: The pEGFP-N1-BTG1 plasmid was constructed successfully, and the expression of BTG1 protein was higher in experimental group than that in control group (0.921±0.091 vs 0.308±0.047, P<0.05). Compared with the two group of laryngeal cancer Hep-2 cells, the cell growth in experimental group was slowed down and the proliferation was reduced (P<0.05); Cyclin D1 protein expression level was decreased (0.436±0.023 vs 0.916±0.092, P<0.05), the proportion of G₀/G₁ phase cell cycle was increased [(85.1±5.2)% vs (63.8±3.1)%, P<0.05], the proportion of S phase cell was decreased [(8.3±1.1)% vs (23.1±1.5)%, P<0.05], phosphatidylserine ectropion in experimental group was increased, cell early apoptosis was significant [(10.3±1.1)% vs (2.8±0.3)%, P<0.05] and anti-apoptotic protein Bcl-2 expression level was reduced(0.167±0.009 vs 0.834±0.084, P<0.05). Conclusion: BTG1 high expression could inhibit the proliferation growth of laryngeal Hep-2 cells and promote its apoptosis, and the possible mechanisms are interrelated with BTG1 involved in cell cycle regulation and causing cell apoptosis.

Key words: Laryngeal cancer, BTG1, Proliferation, Apoptosis, Cell cycle