关键词: 纤维蛋白原, 基因多态性, 结直肠癌

Abstract: Background and purpose: Patients with colorectal cancer are often accompanied by the increase of plasma fibrinogen concentration. This study aimed to investigate the distribution characteristics of beta-fibrinogen gene-448G/A, -148C/T, -1420G/A and -854G/A polymorphism and plasma fibrinogen (Fg) concentration in patients with colorectal cancer. Furthermore, we analyzed their effects on the occurrence and development of cancer. Methods: The level of plasma Fg was quantified by using Clauss clotting method. FGBβ gene polymorphisms were identified by real-time fluorescence quantitative PCR (RTFQ-PCR) in 194 colorectal cancer patients and 74 healthy controls. Results: The plasma Fg levels in tumor metastasis group and non-metastasis group were significantly higher than that in control group, respectively (P<0.05). Compared with control group, the frequencies of -148T allele and mutation genotype were notably higher in disease group (P<0.05). In all the groups, the plasma Fg levels of those with -148T allele were higher than those without -148T allele (P<0.05). In stage Ⅳ patients, there was no difference in PFS between -148T wild genotype group and mutation genotype group (P>0.05). Conclusion: Plasma Fg concentration in patients with colorectal cancer was significantly raised, which suggests that Fg may play a role in the occurrence and development of colorectal cancer. The beta-fibrinogen gene -148C/T polymorphism is one of the reasons that cause plasma Fg elevation, but has no correlation with prognosis of patients with stage Ⅳ colorectal cancer. cancer was significantly raised, which suggests that Fg may play a role in the occurrence and development of colorectal cancer. The beta-fibrinogen gene -148C/T polymorphism is one of the reasons that cause plasma Fg elevation, but has no correlation with prognosis of patients with stage Ⅳ colorectal cancer.

Key words: Fibrinogen, Gene polymorphism, Colorectal cancer