最新刊期
卷 27 ， 期 2 ， 2017
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- 摘要：Background and purpose: Adenosine triphosphate-binding cassette superfamily G member 2 (ABCG2), which has been found over-expressed in a variety of cancer cells, takes part in the drug resistance of cancer through efflux of anticancer drugs. The purpose of this study was to investigate the mechanisms of human glioblastoma cells sensitivity to pyropheophorbide-a methyl ester (MPPa)-mediated photodynamic therapy (PDT) eradicating tumour cells and its relationship to ABCG2. Methods: U87 and A172 glioma cell lines in the logarithmic growth phase were selected and exposed to the treatment of MPPa-PDT and MPPa-PDT+fumitremorgin C (FTC) respectively. The cell viability was measured with the use of CCK-8 assay. The expression of ABCG2 was detected by Western blot. The intracellular contents of MPPa in each group without illumination were tested by flow cytometry. Flow cytometry with Annexin Ⅴ-FITC/PI double staining was used to detect the cell apoptotic rate. DCFH-DA staining was used to assess the generation of intracellular reactive oxygen species (ROS). Results: The MPPa-mediated PDT could eradicate A172 and U87 cancer cells in an energy-dependent manner. The light energy density in A172 was 8 times of that in U87 when the cell viability reached median lethal dose after MPPa-mediated PDT. The high expression of ABCG2 in A172 cells affected the accumulation of intracellular MPPa. Inhibition of ABCG2, not only could enhance the eradicating effect of MPPa-PDT on A172 cells, but also could increase the yield of ROS triggered by MPPa-PDT and the accumulation of intracellular MPPa. Conclusion: The human glioblastoma cell line A172 is insensitive to MPPa-mediated PDT. The mechanism may relate to ABCG2, which decreases the MPPa content in cancer cells through efflux of MPPa, resulting in decline of cytotoxicity.关键词：Adenosine triphosphate-binding cassette superfamily G member 2;Pyropheophorbide-a methyl ester;Photodynamic therapy;Sensitivity;Glioma2|2004|0更新时间：2025-12-31
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Bone marrow mesenchymal stem cells prevent pulmonary tumor metastasis after surgery in a mouse model
摘要：Background and purpose: In recent years, the studies indicated that postoperatively induced myeloid-derived suppressor cells (MDSCs) were qualified with potent proangiogenic and tumor-promotive ability. Bone marrow mesenchymal stem cells (BMSCs) significantly inhibited the induction and proliferation of MDSCs. However, the relationship of MDSCs and tumor metastasis during perioperative period, and whether BMSCs could prevent tumor metastasis through inhibiting MDSCs are not clarified. This study aimed to investigate the change of MDSCs during perioperative period and its correlation with tumor metastasis after surgery, and the influence of BMSCs on the induction of MDSCs and the development of postoperative tumor metastasis. Methods: LLC cells were injected intravenously into C57BL/6 mice. Two hours later, these mice were divided into 4 groups: controls (C group); mice given anesthesia (A group); mice given anesthesia and laparotomy (AL group) and mice given anesthesia, laparotomy, and hepatic lobectomy (ALH group). The AL mice were divided into 2 groups after surgical operation: the AL mice without treatment (ALL group) and the AL mice treated with syngeneic BMSCs (ALB group). The percentage of Gr- 1+CD11b+ cells in peripheral blood mononuclear cells (PBMCs) was detected by flow cytometry. The numbers of metastases on the lung surface were counted on the 14th day after LLC infusion. BMSCs were also co-cultured in vitro with myeloid cells in order to illustrate the effects of BMSCs on the generation of MDSCs. Results: The numbers of lung metastases in AL and ALH group significantly increased as compared with C and A group (P<0.01). The number of lung metastases in ALH group significantly increased as compared with AL group (P<0.05). The percentage of Gr-1+CD11b+ cells in PBMCs during postoperative period significantly increased in AL and ALH group as compared with C and A group, and the percentage of Gr-1+CD11b+ cells in ALH group also significantly increased as compared with AL group. The numbers of lung metastases in AL and ALB group were (38.00±9.57) and (6.54±1.49), the difference was statistically significant (P<0.01) on day 14 after LLC infusion. Meanwhile, the percentage of Gr-1+CD11b+ cells in ALB group significantly decreased as compared with AL1 group. This study also demonstrated that BMSCs inhibited the induction and proliferation of MDSCs from myeloid cells in vitro. Conclusion: Surgery stress induces MDSCs and promotes tumor metastasis. Syngeneic BMSCs could inhibit the generation of MDSCs and further suppress tumor metastasis after surgery.关键词：Mesenchymal stem cell;Myeloid cells;Postoperative period;Neoplasm metastasis2|1957|0更新时间：2025-12-31 - 摘要：Background and purpose: miR-17-92 gene cluster overexpression has been observed in various cancers, such as lung cancer, liver cancer, gastric cancer and prostate cancer. In this study, we established the stable cell line overexpressing miR-17-92 to explore the influence of miR-17-92 on the migration, invasion abilities and cisplatin resistance of the prostate cancer DU145 cells. Methods: miR-17-92 overexpression vectors were constructed. DU145 cells were infected with the viral supernatants produced by Phoenix A packaging system. Real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) was conducted to detect the expression level of miR-17-92 in the cells. The migration and invasion abilities were measured by a real-time xCELLigence system. The scratch healing assay was carried out to investigate the migration abilities. The expression of integrin β1 was detected by Western blot, and the activities of matrix metalloprotein-2 (MMP-2) and matrix metalloprotein-9 (MMP-9) were measured by gelatin zymography experiment. The cell growth of the two cell lines after the treatment of cisplatin was detected by a real-time xCELLigence system. The mRNA expression of ERCC1 was measured by RTFQ-PCR. Western blot was conducted to investigate the protein expressions of ERCC1, ERK1/2 and pERK1/2. Results: DU145-miR-17-92 cells migrated faster than DU145-control cells during the 24 h continuous monitoring (P<0.01). The scratch healing assay indicated that DU145-miR-17-92 cells migrated from the edge towards the scratch center faster than DU145-control cells. DU145-miR-17-92 cells invaded through matrigel markedly faster than DU145-control cells (P<0.01). The protein expression level of integrin β1 and the MMP-9 activities in DU145-miR-17-92 cells were increased than those in DU145-control cells. After the treatment of cisplatin, DU145-miR-17-92 cells grew faster than DU145-control cells, presenting cisplatin resistance (P<0.01). The phosphorylation of ERK1/2 in DU145-miR-17-92 cells was constantly at a high level regardless of the treatment of cisplatin. Compared with DU145-control cells, the expression of drug resistance-related gene ERCC1 was dramatically increased in DU145-miR-17-92 cells after the treatment of cisplatin. Conclusion: miR-17- 92 overexpression increases the migration and invasion abilities of the prostate cancer DU145 cells, which is associated with the upregulated expression of integrin β1 and the increased activity of MMP-9. Besides, miR-17-92 overexpression enhances the cisplatin resistance of DU145, which is correlated with the increased phosphorylation level of ERK and the upregulated expression of ERCC1 at both the mRNA and protein levels.关键词：miR-17-92;Prostate cancer;DU145;Invasion;Migration;Cisplatin2|1558|0更新时间：2025-12-31
- 摘要：Background and purpose: Aneuploidy is associated with tumor formation. This study aimed to study the relationship between aneuploidy and tumorigenicity of tumor cells. Methods: The proliferations of C3 and C5 cell lines were tested by CCK-8 assay. Regular chromosome assay was used to analyze the karyotypes of the 2 cell lines. The tumorigenicity of C3 and C5 cell lines was tested by soft agar colony formation assay in vitro. The invasion and metastasis of C3 and C5 cell lines were detected by transwell chamber. The tumorigenicity of C3 and C5 cell lines was tested by xenograft in vivo. Results: The proliferative ability of C3 was stronger than that of C5. The difference was statistically significant. The karyotype assay showed that the karyptypes of the C3 and C5 cell lines were all aneuploid and their model numbers were 38-78 for C3 cell line and 28-50 for C5 cell line respectively. The proportions of C3 hypodiploid, diploid and hyperdiploid were 20.33%, 8.47%, and 71.2%, respectively. The proportions of C5 hypodiploid, diploid and hyperdiploid were 11.11%, 86.42%, and 2.47 %, respectively. The proportions of aneuploid C3 and C5 were 95.73% and 13.58%, respectively. C3 cell line could form colonies in soft agar, while C5 cell line failed to form colonies. The invasion and metastasis of C3 was stronger than that of C5 in vitro. The results showed that the tumorigenicity of C3 cell line was stronger than that of C5 cell line in vivo. Liver and kidney metastases from C3 were observed, while the C5 only spread to the kidneys. Conclusion: Aneuploidy may not be the only reason for the tumorigenicity of cancer cells. There is a correlation between aneuploidy and tumorigenicity of tumor cells. Aneuploidy contributes to malignant transformation and tumorigenesis of tumor cells.关键词：Aneuploidy;Tumorigenicity;Karyotype;Proliferation;Invasion and metastasis2|2480|0更新时间：2025-12-31
- 摘要：Background and purpose: The incidence of cervical cancer is rather high in Xinjiang, which is closely associated with the infection of human papilloma virus type 16 (HPV-16). The purpose of this study was to analyze the variants and function of HPV-16 upstream regulatory region (URR) in the tissues of cervical cancer biopsies from Xinjiang. Methods: The DNAs were extracted from the tissues of cervical epithelial atypical hyperplasia (CIN) and cervical cancer biopsies. HPV-16 URR segments were amplified by PCR. Based on the sequence analysis of the URR, the representative URR variants were selected and cloned into pGL3-Basic. The recombinant plasmids were transfected into Vero cell lines respetively. Luciferase activity of transfected cells was detected 48 h after transfection. Results: Fifty-five HPV-16 URR DNA fragments were obtained through PCR, and 44 mutations were found from the URR fragments. 4 of these mutations, including nt7192(G→T) , nt7433(- →T), nt7435 (C→G) and nt7863 (A→-) occurred in all sequences. The mutation at nt7520 (G→A) occurred in 54 URR sequences, and the 39 other mutations were present in different samples. Based on the location and frequency of the mutations in the URR fragments, 9 URR variants were selected and cloned into pGL3-Basic. Then the luciferase activity of the cells transfected with pGL3- URR plasmids was detected respectively. Promoter activity of URR mutants from cervical cancer are significantly higher than that of URR mutants from CIN (P<0.01). Promoter activity of URR fragments from some cervical cancer was significantly higher than that of the URR fragments from SiHa and Caski cells. Conclusion: Multiple mutations occurred in HPV-16 URR of cervical cancer patients from Xinjiang. The promoter activity and carcinogenicity of some URR mutants have been enhanced.关键词：Human papillomavirus type 16;Upstream regulatory region;Mutation;Promoter2|2142|0更新时间：2025-12-31
- 摘要：Background and purpose: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. Intrahepatic recurrence is the main factor affecting its medium-term survival rate. Therefore, the search for the markers of metastasis is essential. This study aimed to evaluate the relationship of expression of tyrosine kinase phosphorylation Tyr416 of sarcoma (SRC pY416) in HCC with clinical parameters and prognosis. Methods: Immunohistochemical method and Western blot were used to detect the expression of non-receptor tyrosine kinase (SRC pY416) in 112 cases of HCC tissues and 40 cases of corresponding cancer adjacent normal liver tissues. Hepatitis B virus (HBV) DNA and alpha fetoprotein (AFP) in patients were detected with chemiluminescence. In the 12 months Follow-up of the study, the association between SRC pY416 expression and clinical parameters was analyzed. Results: SRC pY416 expressions in HCC (65.40±15.69) were higher than those in cancer adjacent normal liver tissues (11.25±2.73, P<0.001). The expressions of SRC pY416 were all associated with the age, the liver cirrhosis, the complete capsule, the tumor differentiation, the HBV DNA and the AFP value of the patients (P<0.01). 12 months after operation, single factor analysis showed that the recurrence was associated with the tumor differentiation, the HBV DNA, the AFP value and the expression of SRC pY416 of the patient (P<0.01). Multivariate analysis showed that the expression of SRC pY416 was an independent prognostic factor for recurrence and metastasis in patients with HCC in 12 months. Conclusion: SRC pY416 may play an important role in the metastasis of HCC. The expression of SRC pY416 may be the marker for HCC liver metastasis.关键词：Liver neoplasms;Hepatocellular carcinoma;Tyrosine kinase phosphorylation Tyr416 of sarcoma2|1934|0更新时间：2025-12-31
- 摘要：Background and purpose: Triple negative breast cancer (TNBC) is with high invasion, poor prognosis and lack of usefull treatment. This study investigated expression status of ICAM-1 protein in TNBC in order to explore its relationship with clinicopathological features and outcome in patients. Methods: Fifty-nine tissue samples of TNBC were collected while 50 cases of para-carcinoma tissue samples were used as negative controls. Immunohistochemical staining was conducted to detect expression level of ICAM-1 protein. The relationship of ICAM-1 protein expression with clinicopathological features (age, tumor size, subtype, grade, status of lymph node metastasis, TNM stage, vascular tumor thrombus, nerve infiltration, Ki-67, p53 and E-cadherin expression) and outcome in patients were analyzed. Results: The ICAM-1 protein expression of TNBC was significantly higher than that in adjacent tissues (P=0.000). ICAM-1 expression was related to status of lymph node metastasis, grade and TNM stage (with a P-value of 0.036, 0.027 and 0.048, respectively), while demonstrated an undefined relationship with tumor size, subtype, vascular tumor thrombus and expression of Ki-67, p53 and E-cadherin. The disease-free survival (DFS) of ICAM-1 high expression set was shorter than that of the lower one but has nothing to do with overall survival (OS). In addition, Cox proportional hazards model showed that ICAM-1 expression and lymph node metastasis were independent risk factors of DFS in patients (HR=3.2, 95%CI: 1.6 to 6.4, HR=2.7, 95%CI: 1.28 to 5.9, P<0.05). Conclusion: ICAM-1 could serve as a predictive factor for differentiation status of TNBC. The high expression of ICAM-1 in TNBC may indicate poorer prognosis.关键词：Triple-negative breast cancer;Intercellular adhesion molecule-1;Immunohistochemistry;Prognosis4|3611|0更新时间：2025-12-31
- 摘要：Background and purpose: Radiomics is an emerging field that generates large amounts of valuable clinical information through extracting quantitative imaging features. The purpose of this study was to use the radiomics approach to assess the value of features captured from PET and CT in predicting the therapeutic effect in stage Ⅰ non-small cell lung cancer (NSCLC) after stereotactic ablative radiotherapy (SABR). Methods: Patients with stage Ⅰ NSCLC confirmed by pathology and treated with SABR were included retrospectively. The gross tumor volume (GTV) was defined by two radiologists. PET and CT scan images were collected, and radiomic features were further extracted and analyzed. Non-negative matrix factorization was used to distinguish patients with or without local control. Results: Sixteen patients were eligible for analysis. This study identified two PET features (LL_GLCM_Maximal_Correlation_Coefficient and HL_GLRMS_LRE) captured from PET/CT as having significance in classifying patients with or without disease development. This study not find similar results in CT scans. Conclusion: It seems feasible to use radiomics information effects from PET/CT to predict therapeutic effects of SABR in stage Ⅰ NSCLC. Further investigation is needed.关键词：Radiomics;PET/CT;Lung cancer;Stereotactic ablative radiotherapy2|2421|0更新时间：2025-12-31
- 摘要：Background and purpose: New treatment methods should be explored for non-small cell lung cancer (NSCLC) patients with acquired resistance to epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI). This study compared the curative effect of pemetrexed with gefitinib or pemetrexed alone in advanced NSCLC with acquired resistance to EGFR-TKI. Methods: This study included 62 NSCLC patients with advanced EGFR gene mutation and acquired resistance to EGFR-TKI. Among those, 32 patients were treated with pemetrexed and gefitinib, and 30 patients treated with gefitinib alone. The differences in outcomes between the two strategies were assessed. Results: Objective response rate (ORR) was 46.9% for those treated with pemetrexed and gefitinib and 20% for those treated with pemetrexed alone(χ2=4.933, P<0.05). There was no significant differences between the two groups on disease control rate (DCR) (P>0.05). The median progression-free survival (PFS) was 8.0 months on pemetrexed and gefitinib group and 6.3 months on pemetrexed alone (χ2=8.063, P<0.05). There was no significant differences between the two groups on overall survival (OS) (P>0.05). Higher occurrence of leukocytopenia and rash was observed in the pemetrexed and gefitinib group than in the pemetrexed group (P<0.05). There was no significant differences between the two groups on grade 3-4 toxicities (P>0.05). Conclusion: This study was to demonstrate that continuation of EGFR-TKI with pemetrexed in patients with acquired resistance improves outcomes compared with pemetrexed alone. An improved response rate and PFS were observed in this study. A larger prospective clinical trial is needed to further evaluate this promising strategy.关键词：培美曲塞;吉非替尼;非小细胞肺癌\表皮生长因子受体-酪氨酸激酶抑制剂2|2012|0更新时间：2025-12-31
- 摘要：Background and purpose: The local recurrence is one of the main causes of treatment failure for rectal carcinomas after radical resection. This study aimed to investigate the feasibility, safety, and efficacy of CT guided cryoablation in local recurrence after radical resection of rectal carcinomas. Methods: From Oct. 2013 to Jan. 2015, a total of 22 patients were enrolled in this study, and a follow-up of 12-32 months were performed. The image follow-up was by contrast-enhanced CT or MRI of pelvic. The technical success rate, complications and managements, and 1-year local control rate were recorded. The degree of pain between preablation and postablation was compared by scores of numerical rating scale (NRS). Results: The technical success rate was 100%. The main complication rate was 18.5%, including dysuresia, muscle strength decreased of affected side, abscess formation, severe frostbite. The 1-year local control rate was 72.7%, and the time to progression of local lesion were (11.1±4.3) months. Scores of NRS had significant difference between preablation and 3-day postablation, and preablation and 6-month postablation (P<0.05). There was no significant difference between preablation and 12-month postablation (P=0.854). Conclusion: CT-guided cryoablation as a new method for local recurrence after radical resection of rectal carcinomas was safe and feasible, which had high rate of local control, and relieved pain obviously for a period of time.关键词：Rectal carcinoma;Local recurrence;Cryoablation2|3112|0更新时间：2025-12-31
- 摘要：Papillary thyroid cancer (PTC) is the most common pathological type of thyroid cancer, always with favorable prognosis. However, the incidence of thyroid cancer recently appeared to be an increasing trend. Because of the increased amount of patients, refractory thyroid cancer was not rare anymore. Hence, we are facing a big challenge how to manage and treat the increasing number of patients. BRAF V600E mutation is a classic DNA-relative biomarker for PTC, and widely used in preoperative diagnosis and evaluation of prognosis. As a potential therapeutic target, it attracted more and more attention. Recognizing BRAF V600E mutation can help us to know oncogenesis and biological behavior of PTC better and provide profitable treatment and management.关键词：BRAFV600E mutation;Papillary thyroid cancer;Oncogenesis;Aggressiveness;Prognosis;Survival analysis2|9303|0更新时间：2025-12-31
- 摘要：The WNT/β-catenin signaling pathway plays a critical role in cellular proliferation, differentiation and organogenesis. Aberration activation of WNT/β-catenin pathway and dysregulation of miRNA related with this pathway are involved in oncogenesis and tumor progression in primary lung cancer. Understanding the mechanism of the WNT/β-catenin signaling pathway and illuminating the interaction between miRNA and the members of this pathway may improve the perspectives of using these molecules as potential therapeutic targets for primary lung cancer. This review focused on the participation of the WNT/β-catenin signaling pathway and miRNA in lung cancer and discussion of potential targets for this malignancy therapy in the future.关键词：Lung cancer;WNT/β-catenin signaling pathway;miRNA2|2457|0更新时间：2025-12-31
- 摘要：With the development of science and technology, the diagnosis and treatment of tumor is more and more advanced, which greatly prolongs patients’ survival time and improves the quality of life leading to increased incidence and detection rate of multiple primary malignant tumors. However, misdiagnosis of multiple primary malignant tumors increases the mortality rate of patients. In this review, we mainly discussed the overview, epidemiology, etiology, diagnosis, treatment and prognosis of multiple primary malignant tumors.关键词：Multiple primary malignant tumors;Etiology;Diagnosis2|3738|0更新时间：2025-12-31
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