δ-catenin与Cdc42在非小细胞肺癌中表达的相关性

doi:10.3969/j.issn.1007-3969.2016.03.004

中国癌症杂志 ›› 2016, Vol. 26 ›› Issue (3): 221-229.doi: 10.3969/j.issn.1007-3969.2016.03.004

δ-catenin与Cdc42在非小细胞肺癌中表达的相关性

张　迪^1,2, 王恩华^1,2

1. 中国医科大学基础医学院病理教研室，辽宁沈阳110122 ；
2. 中国医科大学附属第一医院病理科，辽宁沈阳110001

出版日期:2016-03-30 发布日期:2016-06-13
通信作者: 王恩华　E-mail:wangeh@hotmail.com
基金资助:
国家自然科学基金(81101779)。

Correlation between δ-catenin and Cdc42 expression in non-small cell lung cancer

ZHANG Di^1,2, WANG Enhua^1,2

1.Department of Pathology, the College of Basic Medical Sciences, China Medical University, Shenyang 110122, Liaoning Province, China; 2. Department of Pathology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China

Published:2016-03-30 Online:2016-06-13
Contact: WANG Enhua E-mail: wangeh@hotmail.com

摘要/Abstract

摘要： 背景与目的：δ-catenin是p120 catenin 亚家族中的成员，可与细胞膜上的E-cadherin直接结合，形成E-cadherin/catenin复合体。δ-catenin还可以通过调节Cdc42(Small GTP酶)活性以影响细胞骨架装配。该研究检测非小细胞肺癌(non-small cell lung cancer，NSCLC)中δ-catenin及Cdc42的表达情况并探讨了二者表达的相关性。方法：采用免疫组织化学方法检测122例NSCLC标本中δ-catenin与Cdc42的表达。采用蛋白［质］印迹法(Western blot)及逆转录聚合酶链反应(reverse transcription polymerase chain reaction，RT-PCR)法检测肺癌组织中δ-catenin及Cdc42的蛋白及mRNA表达情况。在肺癌细胞系中，分别上调或干扰δ-catenin的表达后，利用G-LISA及Transwell小室法检测Cdc42活性以及肺癌细胞侵袭能力的改变。结果：δ-catenin和Cdc42在肺癌组织中其蛋白及mRNA表达明显高于正常肺组织。而在122例NSCLC病例中，δ-catenin阳性表达率为65.57%(80/122)，Cdc42过表达率为68.03%(83/122)。δ-catenin阳性表达和Cdc42的过表达具有较好的相关性(P<0.001)。δ-catenin和Cdc42的协同表达与肺癌的高临床分期、低分化、病理类型为腺癌和淋巴结转移相关(P<0.05)，并且与肺癌患者的不良预后明显相关。在肺癌细胞系中通过调节δ-catenin表达，改变Cdc42的表达及活性，影响肺癌细胞的侵袭能力。结论：在肺癌组织中δ-catenin和Cdc42的表达具有相关性，而二者协同表达与患者不良预后相关。

关键词: 　δ-catenin, Cdc42, Small GTP酶, 非小细胞肺癌, 预后

Abstract: Background and purpose: δ-catenin is a member of the p120 catenin subfamily, which can directly bind to E-cadherin on the cell membrane, forming E-cadherin/catenin complex. δ-catenin can also affect the cytoskeleton assembly by regulating the activity of Cdc42 (Small GTPase). Therefore, this study detected the expression of δ-catenin and Cdc42 in non-small cell lung cancer (NSCLC) and investigated the relationship between them. Methods: The expressions of δ-catenin and Cdc42 in 122 cases of NSCLC were detected by immunohistochemistry. This study also used Western blot and real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) to detect the protein and mRNA expressions of δ-catenin and Cdc42 in lung cancer tissues. After up-regulating or downregulating δ-catenin in lung cancer cell line, the activity of Cdc42 and invasion ability of lung cancer cells were detected by G-LISA and Transwell. Results: The mRNA and protein expression of δ-catenin and Cdc42 in lung cancer tissues was significantly higher than that in normal lung tissues. In 122 NSCLC cases, the δ-catenin positive expression rate was 65.57% (80/122), and the Cdc42 overexpression rate was 68.03% (83/122). There was a good correlation between δ-catenin positive expression and Cdc42 overexpression (P<0.001). The co-expression of δ-catenin and Cdc42 was related to the high clinical stage, poor differentiation, adenocarcinoma and lymph node metastasis of lung cancer (P<0.05), and was significantly associated with poor prognosis in patients with lung cancer. In the lung cancer cell line, the expression and the activity of Cdc42 were changed by regulating the δ-catenin expression, which affected invasion ability of the lung cancer cells. Conclusion: The δ-catenin expression was significantly correlated with the Cdc42 expression. The co-expression of δ-catenin and Cdc42 in lung cancer was correlated with the poor prognosis of lung cancer.

Key words: δ-catenin, Cdc42, Small GTPase, Non-small cell lung cancer, Prognosis

张　迪,王恩华. δ-catenin与Cdc42在非小细胞肺癌中表达的相关性[J]. 中国癌症杂志, 2016, 26(3): 221-229.

ZHANG Di, WANG Enhua. Correlation between δ-catenin and Cdc42 expression in non-small cell lung cancer[J]. China Oncology, 2016, 26(3): 221-229.

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δ-catenin与Cdc42在非小细胞肺癌中表达的相关性

Correlation between δ-catenin and Cdc42 expression in non-small cell lung cancer

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