Abstract: Background and purpose: At present, the incidence of primary breast cancer in female populations in China is gradually increasing, which has a great impact on women's physical and mental health. Therefore, the characteristics of malignant calcified mammography and their correlation with expressions of vascular endothelial growth factor (VEGF) and E-cadherin in primary breast cancer were systematically analyzed to provide targeted guidance for clinical diagnosis and treatment. Methods: A retrospective analysis of 243 patients with primary breast cancer confirmed by surgery and pathology was performed in Changzhou Second People’s Hospital from Mar. 2015 to Apr. 2019. All patients received mammography before surgery. And after the operation, pathological and immunohistochemical examinations were carried out, and the surgical and pathological results were used as the gold standard to analyze the diagnosis results of this group. Results: In this group of 243 patients, there were 118 cases with malignant calcification and 125 cases without malignant calcification. The results showed that the Twist protein was significantly correlated with breast cancer malignant calcification, morphology and quantity. Besides, the Twist protein expression was positively correlated with the VEGF expression in breast cancer tissues (P<0.05), and the positive expression of Twist protein was negatively correlated with the positive expression of E-cadherin (P<0.05). Conclusion: The expressions of Twist protein, VEGF and E-cadherin can provide a biological basis for the diagnosis of malignant calcified molybdenum and palladium in breast cancer, which has clinical significance.

Key words: Primary breast cancer, Malignant calcification, Molybdenum target mammography, Twist protein, Vascular endothelial growth factor, E-cadherin