Abstract: Background and purpose: It was reported that Vav3 gene was expressed aberrantly in many cancers. But the relationship between Vav3 and angiogenesis genes of gastric cancer is still not clear. The purpose of this research was to investigate the effect and significance of Vav3 to angiogenesis of human gastric cancer cell line BGC823. Methods: The expressions of Vav3 in human gastric cancer cell line BGC823, gastric epithelial cell line GES-1 were tested by fluorecence quantitative RT-PCR and Western blot. Then Vav3-siRNA was synthesized and transfected into BGC823. Activity of BGC823 was measured with fluorecence quantitative RT-PCR and MTT assay. The expressions of angiogenesis related genes VEGF-A, VEGF-C, VEGF-D, angiogenin-1 (Ang-2), vasohibin-1 were determined by qPCR and Western blot. Results: Expressions of Vav3 were detected higher in gastric cancer BGC823 cells than in GES-1 (P<0.01). Expression of Vav3 was inhibited by Vav3-siRNA (P<0.05). Activity of BGC823 was obviously inhibited after Vav3-siRNA transfected with MTT assay (P<0.05). The expressions of VEGF-C, Ang-2 were lower in cells after transfected by Vav3-siRNA(both P<0.05); expression of Vasohibin-1 increased after Vav3-siRNA transfected into BGC823 (P<0.05). Conclusion: Vav3 gene can regulate some angiogenesis related genes, and Vav3 may promote angiogenesis of gastric cancer cells with this fuction.

Key words: Gastric cancer, Vav3 gene, Angiogenesis, RNA interference