Abstract: Background and purpose: Cervical cancer is the fourth most common cancer in females worldwide. In recent years, the morbidity and mortality are rising in China. The continued infection with high-risk human papillomavirus (hrHPV) is closely related to the invasive cervical carcinoma (ICC) and the precancerous lesions. The application of HPV prophylactic vaccine has become an effective measure for the prevention of cervical cancer. This study aimed at providing more evidence for the estimation of the potential impact of 9-valent HPV vaccine, by analyzing the distribution of 7 hrHPV types (HPV16/18/31/33/45/52/58), included in the vaccine, in histologyconfirmed ICCs and precancerous lesions from local women. Methods: Cervical samples with histology follow-up from Fudan University Shanghai Cancer Center were collected for HPV genotyping by polymerase chain reaction-reverse dot blot (PCR-RDB). Relative contribution of HPV types in ICCs and precancerous lesions was expressed as the percentages of type-specific cases among HPV-positive cases. Multiple infections were incorporated into single infection using the method of proportional weighting attribution (PWA). Results: A total of 624 cases were obtained, including 117 cases of negative for intraepithelial lesion or malignancy (NILM), 45 low-grade squamous intraepithelial lesion (LSIL), 268 high-grade squamous intraepithelial lesion (HSIL) and 194 ICC. The HPV prevalences of all, and of LSIL, HSIL and ICC were 84.29%, 71.11%，95.90% and 91.75%, respectively. The PWA of HPV 16/18 to LSIL, HSIL and ICC were 50.00%, 70.44% and 91.71%, whereas the attribution of HPV16/18/31/33/45/52/58 to LSIL, HSIL and ICC were increased to 86.34%，98.61% and 94.89%, respectively. The addition of 5 hrHPV types (HPV31/33/45/52/58) resulted in the reduction of relative contribution in LSIL, HSIL and ICC, as 36.34%, 28.17% and 3.18%, respectively. Conclusion: With the inclusion of additional 5 hrHPV types, the 9-valent HPV vaccine would likely prevent the occurrence of more ICC and precancerous lesions, and would especially benefit the prevention of LSIL and HSIL in China.

Key words: Genotype, 9-valent human papillomavirus vaccine, Squamous intraepithelial lesion, uterine cervical cancer