中国癌症杂志 ›› 2017, Vol. 27 ›› Issue (4): 262-267.doi: 10.19401/j.cnki.1007-3639.2017.04.004

• 论著 • 上一篇    下一篇

干扰FBI-1表达对人乳腺癌细胞增殖的影响及其作用机制研究

王 丽,覃庆洪,谭启杏,练 斌,杨伟萍,韦长元   

  1. 广西医科大学附属肿瘤医院乳腺外科,广西 南宁 530021
  • 出版日期:2017-04-30 发布日期:2017-05-05
  • 通信作者: 韦长元 E-mail: weicy63@aliyun.com
  • 基金资助:
    广西自然科学基金项目(2015GXNSFAA139204);国家自然科学基金(81360396)。

Effect of downregulation of FBI-1 on proliferation of human breast carcinoma cell line and its mechanism

WANG Li, QIN Qinghong, TAN Qixing, LIAN Bin, YANG Weiping, WEI Changyuan   

  1. Department of Breast Surgery, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Online:2017-04-30 Published:2017-05-05
  • Contact: WEI Changyuan E-mail: weicy63@aliyun.com

摘要: 背景与目的:人类免疫缺陷病毒短转录诱导物连接因子1(factor that binds to the inducer of short transcripts of human immunodeficiency virus-1,FBI-1)在多种恶性肿瘤中都高表达,可能与肿瘤增殖分化、血管发生及转移等生物学行为密切相关,其与乳腺癌的关系尚未完全阐明。该研究旨在探讨FBI-1在乳腺癌细胞中的表达,并研究靶向干扰FBI-1基因表达对乳腺癌细胞增殖的影响及其可能的作用机制。方法:应用实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,RTFQ-PCR)和蛋白[质]印迹法(Western blot)检测人正常乳腺上皮细胞株MCF-10A、人乳腺癌细胞株MCF-7中FBI-1的表达水平;采用shRNA干扰技术抑制MCF-7细胞中FBI-1基因的表达,采用CCK-8增殖实验及平板克隆形成实验检测细胞增殖能力,采用RTFQ-PCR和Western blot检测干扰FBI-1表达前后MCF-7细胞中FBI-1及NF-κBp65的表达。结果:FBI-1mRNA及蛋白在人乳腺癌细胞中高表达(P<0.05)。采用shRNA干扰技术抑制MCF-7细胞的FBI-1表达后,MCF-7细胞的增殖能力明显减弱(P<0.05);同时,抑制FBI-1表达后,NF-κBp65的mRNA及蛋白表达水平均显著下降(P<0.05)。结论:FBI-1在乳腺癌细胞中高表达,下调FBI-1的表达可以抑制乳腺癌细胞的增殖,其作用机制可能与抑制NF-κB信号通路有关。

关键词: 人类免疫缺陷病毒短转录诱导物连接因子1, 乳腺癌, 细胞增殖, NF-&kappa, B

Abstract: Background and purpose: Factor that binds to the inducer of short transcripts of human immunodeficiency virus-1 (FBI-1) in a variety of malignant tumors showed high expression levels, which may be closely related to tumor proliferation and differentiation, angiogenesis, metastasis, but its relationship with breast cancer has not been fully elucidated. The purpose of this study was to investigate the expression of FBI-1 in breast cancer cells, and to study the effect of FBI-1 gene expression on the proliferation of breast cancer cells and its possible mechanism. Methods: Real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot analysis were applied to detect FBI-1 expression in normal human mammary epithelial cell line MCF-10A and breast cancer cell MCF-7. RNA interference method was used to down-regulate FBI-1 expression in MCF-7 cells. The cell proliferation was measured by CCK-8 kit and colony formation assay. RTFQ-PCR and Western blot were used to detect the expression of FBI-1 and NF-κBp65 in MCF-7 cells before and after the interference of FBI-1 expression. Results: The expression of FBI-1 was higher in breast cancer cells than that in normal human mammary epithelial cells (P<0.05). The effects of FBI-1 down-regulation inhibited proliferation in MCF-7 cells (P<0.05). At the same time, after inhibition of FBI-1, the NF-κBp65 mRNA and protein expression levels were significantly decreased (P<0.05). Conclusion: FBI-1 is highly expressed in breast cancer cells. Down-regulated FBI-1 expression can inhibit the proliferation of breast cancer cells, and its mechanism may be related to the inhibition of NF-κB signaling pathway.

Key words: Factor that binds to the inducer of short transcripts of human immunodeficiency virus-1, Breast cancer, Cell proliferation, NF-κB