China Oncology ›› 2023, Vol. 33 ›› Issue (2): 152-161.doi: 10.19401/j.cnki.1007-3639.2023.02.008
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LENG Jie(), QIU Guochun, ZHANG Bo, PU Yan
Received:
2022-08-03
Revised:
2022-12-03
Online:
2023-02-28
Published:
2023-03-22
Contact:
LENG Jie
CLC Number:
LENG Jie, QIU Guochun, ZHANG Bo, PU Yan. Mechanism of breast cancer centrosome regulatory protein SEC23B on tumor invasion and metastasis[J]. China Oncology, 2023, 33(2): 152-161.
Fig. 1
SEC23B is upregulated in metastatic breast cancer A: Kaplan-Meier survival analysis of the correlation between SEC23B expression levels and overall survival of breast cancer patients was performed in an online database (n = 4 929); B: Association between SEC23B expression and breast cancer metastasis was analyzed using the HCMDB database box line (n = 368); C: SEC23B expression in breast cancer cells was detected by Western blot."
Fig. 2
SEC23B promotes breast cancer migration in vitro A: Transfection efficiency of SEC23B overexpression plasmid in MCF-7 cells was examined by Western blot; B-C: Effect of SEC23B overexpression on MCF-7 cell migration was assessed by transwell assay and wound healing assay; D: The expression of sh-SEC23B lentivirus in MDA231-LM2 cells by Western blot; E-F: Effect of SEC23B knockdown on migration of MDA231-LM2 cells was assessed by transwell assay and wound healing assay. ***: P<0.001, compared to control or sh-NC groups."
Fig. 3
SEC23B induces autophagy in breast cancer cells A: Effect of SEC23B overexpression plasmid on p62, LCA/B expression in MCF-7 cells detected by Western blot; B: Representative images and quantitative analysis of the effect of SEC23B overexpression on autophagic granules in MCF-7 cells detected by immunofluorescence staining; C: Effect of SEC23B knockdown on p62, LCA/B expression in MDA231-LM2 cells by Western blot; D: Representative images and quantitative analysis of the effect of SEC23B knockdown on autophagic granules in MDA231-LM2 detected by immunofluorescence staining; E: Effect of CQ on migration of SEC23B overexpressing MCF-7 cells was assessed by transwell assay. **: P<0.01, compared with control or sh-NC groups; ***: P<0.001, compared with control or sh-NC groups."
Fig. 4
ERK/mTOR pathway is involved in SEC23B-induced autophagy in breast cancer cells A: Effect of SEC23B overexpression plasmid on ERK/mTOR pathway expression in MCF-7 cells detected by Western blot; B: Effect of SEC23B knockdown on ERK/mTOR pathway expression in MDA231-LM2 cells detected by Western blot; C: Effect of EGF on ERK/mTOR pathway expression in MCF-7 cells detected by Western blot, effects of EGF on ERK/mTOR pathway and p62, LCA/B expression in SEC23B overexpressing MCF-7 cells detected by Western blot; D: Representative images and quantitative analysis of the effects of EGF on autophagic granules in SEC23B overexpressing MCF-7 cells detected by immunofluorescence staining. **: P<0.01, compared with the control group; #: P<0.05, compared with the SEC23B group."
Fig. 5
SEC23B knockdown inhibits breast cancer metastasis and autophagy in vivo A: Tumors were excised 21 days after implantation and final tumor weight was recorded. B: Histological examination by H-E staining was performed to determine tumor morphology and structural changes. Tumor necrosis was indicated by a red arrow. C: Lung metastases were examined by H-E staining. Tumor metastases in lung tissue were indicated by yellow arrows. D: Lung tissue was excised and fixed in Bouin solution 21 days after implantation, and the number of metastatic nodules was recorded for each mouse. Metastatic nodules were indicated by black arrows. E: LC3 expression in tumor tissues was examined by H-E staining. **: P<0.01, compared with sh-NC group; ***: P<0.001, compared with sh-NC group."
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