Abstract: Background and purpose: High expression of F2R like thrombin or trypsin receptor 3 (F2RL3) is associated with poor prognosis of renal cancer. However, the function of F2RL3 involved in development of renal cancer has not been reported. The aim of this paper was to investigate the correlation of F2RL3 with proliferation, invasion, metastasis and glycolysis in renal cancer A498 cells. Methods: By knockdown of the expression of F2RL3 gene in renal cancer A498 cells, the effects of F2RL3 on the proliferation, invasion, metastasis, glucose uptake, lactate production and the expression of key glycolytic genes were detected. Results: Decreased F2RL3 inhibited the proliferation, invasion and metastasis of renal cancer A498 cells, and suppressed the glycolytic effect of renal cancer A498 cells by inhibiting glucose uptake and lactate production (P<0.005). Conclusion: F2RL3 positively regulated the proliferation, invasion, metastasis and the transformation of glucose metabolism in renal cancer A498 cells. These results provide important theoretical basis for studying the association of the invasion and metastasis of renal cancer with glucose metabolism.

Key words: Renal cancer, F2R like thrombin or trypsin receptor 3, Invasion, Metastasis, Glycolysis