Fig. 1

The expression of YTHDF2 is up-regulated in GBM tissue and cells A: Results predicted by GEPIA website; B: Results predicted by GSCA website; C: CGGA database prediction results; D, E: The expression level of YTHDF2 in GBM tissue and adjacent normal tissue was detected by RTFQ-PCR and Western blot (n=40); F: The expression level of YTHDF2 in GBM cells (LN229 and U251) and NHAs cells was detected by RTFQ-PCR. *: P<0.05, compared with Normal group; &: P<0.05, compared with NHAs cell group."

Fig. 2

Knockdown of YTHDF2 can induce GBM cell cycle arrest and apoptosis A: The knockdown efficiency of YTHDF2 was detected by RTFQ-PCR; B. C: Cell cycle distribution was detected by flow cytometry; D, E: The level of apoptosis rate was detected by flow cytometry. *: P<0.05, compared with si-NC group."

Fig. 3

YTHDF2 promotes the degradation of IGFBP7 via recognizing m6A modified IGFBP7 A: Intersection of mRNA that have binding sites with YTHDF2 and genes that negatively correlated with YTHDF2; B: The expression level of IGFBP7 in GBM tissue and adjacent normal tissue was detected by RTFQ-PCR (n=40); C: The expression level of IGFBP7 in GBM (LN229 and U251) cells and NHAs cells was detected by RTFQ-PCR; D: Results predicted by RMVAR database; E: RIP test results; F-H: RTFQ-PCR was used to detect the mRNA and protein expression of IGFBP7 in GBM cells in si-NC and si-YTHDF2 group; I: The stability of IGFBP7 mRNA was detected. *: P<0.05, compared with Normal group; &: P<0.05, compared with NHAs cell group; #: P<0.05, compared with IgG group; ^: P<0.05, compared with si-NC group."

Fig. 4

Downregulation of IGFBP7 could partially rescue the regulatory effects of YTHDF2 knockdown on GBM cell cycle distribution and apoptosis A: The expression of IGFBP7 was detected by RTFQ-PCR; B. C: cell cycle distribution was detected by flow cytometry; D. E: The level of apoptosis was detected by flow cytometry. ^: P<0.05, compared with si-NC group; $: P<0.05, compared with si-YTHDF2+shNC group."

Fig. 5

YTHDF2 induces PI3K/AKT activation by inhibiting IGFBP7 A: GEPIA website forecast results; B: Prediction results from Linkedomics website; C-F: The protein expression level of p-AKT, AKT, p-PI3K and PI3K was detected by Western blot. TPM: Transcripts per million. *: P<0.05, compared with oe-NC group; &: P<0.05, compared with shNC group; ^: P<0.05, compared with si-NC group; $: P<0.05, compared with si-YTHDF2+shNC group."