Abstract: Background and purpose: LINC00152 is a long non-coding RNA (lncRNA) that is abnormally expressed in malignant tumors such as liver cancer, gastric cancer and colon cancer. LINC00152 is involved in biological activities such as proliferation, migration and apoptosis. This study mainly investigated the role and mechanism of LINC00152 in temozolomide (TMZ)-induced gliosis of glioblastoma stem cell (GSC). Methods: Glioma U251 cells were cultured in the neural stem cell culture medium, and the GSC growing into the sphere were extracted as a research object. The expression level of the model cell LINC00152 was externally up-regulated using the lentiviral transfection method, and we screened the stably expressed cell line with puromycin. The expression level of LINC00152 was detected by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). The cell viability was detected by cell counting kit-8 (CCK-8) method. The cell cycle was detected by flow cytometry. The expressions of P53, Cyclin A, CDC25A and CDK2 proteins were detected by Western blot. Results: The extracted GSC grew in a spherical shape, and the cell spheres CD133 and Oct-4 were positively expressed. Lentiviral transfection significantly up-regulated the expression level of LINC00152 in GSC (P<0.000 1). The treatment of TMZ (200 μg·mL -1 ) for 48 h significantly reduced the viability of GSC (P<0.000 1), while the overexpression of LINC00152 was resistant to the induction effect of TMZ compared with the no-load control (P<0.000 1). TMZ treatment for 48 h significantly increased the S phase ratio of GSC (P<0.000 1), while LINC00152 overexpression was resistant to TMZ induction compared with the empty control (P<0.000 1). The expressions of Cyclin A, CDC25A and CDK2 in GSC were significantly down-regulated by TMZ for 48 h (P<0.000 1), while the expression of P53 protein was up-regulated (P<0.000 1). Compared with the empty control, overexpression of LINC00152 was resistant to the induction of TMZ (P<0.000 1). Conclusion: Overexpression of lncRNA LINC00152 inhibited TMZ-induced up-regulation of P53 protein expression and down-regulation of Cyclin A, CDC25A and CDK2 protein expressions, and antagonized S phase arrest of GSC.

Key words: Long non-coding RNA, LINC00152, Temozolomide, Cell cycle, Glioma