Fig. 1

Expression of PCIF1 in gastric cancer tissues and cells A: The expressions of PCIF1 in gastric cancer tissues and normal control tissues were evaluated by GEPIA database; B: The relative expression levels of PCIF1 in para-cancerous tissues (P) and gastric cancer tissues (T) were examined by Western blot; C: The relative expression levels of PCIF1 in gastric cancer tissues and non-gastric cancer tissues were examined by RTFQ-PCR; D: GEPIA database analysis revealed that the high expression of PCIF1 indicated that the overall survival prediction of gastric cancer patients was better; E: The expression level of PCIF1 in gastric cancer cell lines was examined by Western blot. **: P<0.01, compared with para-cancerous tissues."

Fig. 2

Knockout PCIF1 inhibits the proliferation, migration and invasion of gastric cancer in vitro A: RTFQ-PCR was used to evaluate the expression of PCIF1 knocked down by shRNA in SNU5 cells; B: CCK-8 assay was performed to evaluate the viability of SNU5 cells silenced by PCIF1; C: EdU assay was performed to evaluate the proliferation ability of SNU5 cells silenced by PCIF1; D-F: The effect of PCIF1 knock-down on migration and invasion of SNU5 cells was detected by transwell assay. *: P<0.05, compared with sh-NC group; **: P<0.01, compared with sh-NC group; ***: P<0.001, compared with sh-NC group."

Fig.3

PCIF1 promotes the proliferation, migration and invasion of gastric cancer in vitro A: RTFQ-PCR was used to evaluate the expression of PCIF1 upregulated by lentivirus in AGS cells; B: CCK-8 assay was performed to evaluate the activity of AGS cells up-regulated by PCIF1; C: EdU assay was performed to evaluate the proliferation ability of AGS cells up-regulated by PCIF1; D-F: Transwell was used to detect the effect of PCIF1 upregulation on the migration and invasion of AGS cells. *: P<0.05, compared with NC group;**: P<0.01, compared with NC group; ***: P<0.001, compared with NC group."

Fig. 4

PCIF1 regulates the expression of ACOT8 in gastric cancer A: The correlation between PCIF1 and ACOT8 expression was analyzed by GEPIA database. B: RTFQ-PCR was used to evaluate the effect of overexpressing PCIF1 on ACOT8 expression in AGS cells (***: P<0.001, compared with NC group). C: RIP-qPCR was used to detect the enrichment of PCIF1 combined with ACOT8 in AGS cells (*: P<0.05, compared with IgG in NC group; #: P<0.05, compared with sh-PCIF1 in NC group). D: Overexpression of PCIF1 prolonged the half-life of ACOT8 mRNA in AGS cells (*: P<0.05, compared with NC group)."

Fig. 5

PCIF1 exerts its tumor-promoting effect through ACOT8 A: RTFQ-PCR was used to confirm the efficiency of sh-ACOT8 in PCIF1 overexpressing AGS cells; B: CCK-8 assay was performed to assess the effect of ACOT8 silencing on the proliferative capacity of PCIF1 overexpressing AGS cells; C: EdU analysis of the effect of ACOT8 silencing on the proliferation of PCIF1 overexpressing AGS cells; D-F: Transwell assessment of the effect of ACOT8 silencing on migration and invasion of PCIF1 overexpressing AGS cells. *: P<0.05, compared with NC group;**: P<0.01, compared with NC group;***: P<0.001, compared with NC group; #: P<0.05, compared with PCIF1 group; ##: P<0.01, compared with PCIF1 group; ###: P<0.001, compared with PCIF1 group."

Fig. 6

PCIF1 promotes the growth of gastric cancer in vivo A: Representative tumors after transplantation of PCIF1 overexpression or negative control AGS cells into nude mice; B: Comparison of tumor volumes in PCIF1 overexpressing or negative AGS cell transplanted tumor nude mice; C: Comparison of tumor weights; D: Expression levels of PCIF1 and ACOT8 in tumor tissues were determined using immunohistochemistry. *: P<0.05, compared with NC group; **: P<0.01, compared with NC group."