The relationship between genetic characteristics and clinical characteristics and the efficacy of 131I therapy in children and adolescents with locally advanced or metastatic differentiated thyroid cancer

doi:10.19401/j.cnki.1007-3639.2022.05.002

Abstract

Abstract:

Background and purpose: The molecular characteristics of differentiated thyroid cancer (DTC) in children and adolescents and its role in clinical practice remain unclear. This study intended to explore the genetic characteristics and its relationship with clinical characteristics and efficacy of ¹³¹I therapy in locally advanced or metastatic pediatric and adolescent DTC patients. Methods: A thyroid cancer-related gene panel (ThyroLead®) was used to test the primary tumors from children and adolescents with DTC treated in Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College from December 2020 to July 2021. The samples were sequenced, the clinicopathological characteristics and ¹³¹I treatment history were retrospectively collected, and the relationship between their genetic characteristics and clinicopathological characteristics and the efficacy of ¹³¹I therapy was analyzed. Results: All the 39 children present locally advanced or metastatic disease. All the children with accessible data had lymph node metastasis, 91.4% (32/35) of whom had lateral lymph node metastasis, and 61.5% (24/39) patients had distant metastasis. Thyroid cancer-related gene variants were detected in 61.5% (24/39) of the patients, among which RET fusion (38.5%, 15/39) and BRAF V600E point mutation (12.8%, 5/39) were the most common. No statistically significant differences were found in clinical characteristics between the mutation group and the non-mutation group (P>0.05). Most of the children (91.7%, 22/24) with distant metastases remained structural incomplete response (SIR) status after ¹³¹I treatment. Nine patients were considered radioactive iodine-refractory (RAIR) status, and 8 of them had thyroid cancer driver mutations, among which NCOA4/RET accounted for 62.5% (5/8). Among cases with RET genetic variations, NCOA4/RET fusion-positive patients tended to present more distant metastasis than those with other forms of RET fusion (33.3% vs 88.9%, P=0.089). Conclusion: Compared with point mutations, fusion gene mutations, especially RET fusions, are more common in locally advanced or metastatic pediatric and adolescent DTC patients. NCOA4/RET fusion-positive tumor has more aggressive behaviors and are more likely to become RAIR.

Key words: Pediatric and adolescent thyroid cancer, Locally advanced, Metastases, Genetic alterations, RET fusions, Radioactive iodine-refractory

CLC Number:

R736.1

SUN Di, SUN Yuqing, ZHANG Xin, HUANG Lisha, LIN Yansong. The relationship between genetic characteristics and clinical characteristics and the efficacy of ¹³¹I therapy in children and adolescents with locally advanced or metastatic differentiated thyroid cancer[J]. China Oncology, 2022, 32(5): 380-387.

Figures/Tables 4

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Tab. 2

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References 28

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Clinical characteristic	All patients	Mutation negative	Mutation positive	P value	U value
Age at diagnosis/year median (IQR)	12.1 (9.1-16.2)	11.9 (7.3-14.1)	13.1 (10.0-16.8)	0.172	228.000
Adolescence or not				0.323^*
Children	21	10	11
Adolescent	18	5	13
Gender				0.742^*
Male	18	6	12
Female	21	9	12
Number of surgery median (IQR)	2 (1-2)	1 (1-2)	2 (1-2)	0.618	198.000
Tumor size D/cm median (IQR)	2.7 (1.5-3.9)	3.0 (1.4-3.5)	2.5 (1.5-4.0)	0.558	130.500
Histology				1.000^*
PTC	38	15	23
FTC	1	0	1
T stage				0.471	145.500
T₁+T₂	16	7	9
T₃+T₄	17	5	12
NA	6	3	3
N stage				0.601	158.500
N_1a	3	2	1
N_1b	32	11	21
NA	4	2	2
M stage				1.000^*
M₀	15	6	9
M₁	24	9	15
Number of RAI therapy median (IQR)	2 (1-2)	1 (1-3)	2 (1-2)	0.943	177.500
Cumulative RAI dose D/mCi median (IQR)	180 (125-300)	160 (70-225)	260 (150-300)	0.202	207.000
RAIR or not				0.228^*
No	30	11	19
Yes	9	1	8
Follow-up period t/month median (IQR)	40.0 (21.0-57.0)	36.0 (21.0-72.0)	41.5 (23.0-56.8)	0.700	193.500
ATA response classification at last follow-up				0.853	186.500
ER	5	2	3
IDR	8	3	5
BIR	4	2	2
SIR	22	8	14

No.	Age at diagnosis/year	Gender	Number of surgery	Tumor size D/cm	Histology (subtype)	T stage	N stage	M stage (site)	Number of RAI therapy	Cumulative RAI dose D/mCi	Follow-up period t/month	Classification of RAIR	Genetic variation
1	16.0	Male	2	4	PTC (classic)	3b	1b	1 (lung)	2	270	51	4^*	NCOA4/RET fusion
2	7.0	Male	1	2.8	PTC (follicular)	3b	1b	1 (lung)	2	250	57	4^*	TP53 R213E mutation, NCOA4/RET fusion
3	13.2	Female	1	4.0	PTC (NA)	2	1b	1 (lung)	3	425	43	4^*	NCOA4/RET fusion
4	14.8	Male	2	3.5	PTC (classic)	2	1b	1 (lung)	2	300	52	4^*	NCOA4/RET fusion
5	11.9	Female	3	2.5	PTC (NA)	4a	1b	1 (lung）	2	270	45	2^#	NCOA4/RET fusion
6	8.9	Male	2	2.5	PTC (DSV)	2	1b	1 (lung)	5	280	78	4^*	CCDC6/RET fusion
7	9.1	Female	1	1.5	PTC (classic)	3b	1b	1 (lung)	2	165	40	1^△	TFG/NTRK1 fusion
8	16.8	Male	3	4.5	PTC (classic)	4b	1b	1 (lung & bone)	2	180	134	1^△	BRAF V600E mutation
9	11.2	Female	2	1.4	PTC (NA)	1b	1b	1 (lung)	3	315	106	1^△	negative

The relationship between genetic characteristics and clinical characteristics and the efficacy of ¹³¹I therapy in children and adolescents with locally advanced or metastatic differentiated thyroid cancer

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