China Oncology ›› 2022, Vol. 32 ›› Issue (9): 827-835.doi: 10.19401/j.cnki.1007-3639.2022.09.010
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LÜ Ye1(), YING Yexia2, YANG Wenjing1, HOU Yujin1, YUAN Chunxiu1, CAO Xiangmei2()
Received:
2022-01-07
Revised:
2022-06-01
Online:
2022-09-30
Published:
2022-10-24
Contact:
CAO Xiangmei
CLC Number:
LÜ Ye, YING Yexia, YANG Wenjing, HOU Yujin, YUAN Chunxiu, CAO Xiangmei. The effect of DZNep on tumor microenvironment through regulating exosomes derived from invasive breast carcinoma[J]. China Oncology, 2022, 32(9): 827-835.
Fig. 1
Changes of Exosomes in Breast Cancer before and after DZNep Treatment A: Compared with the control group, the molecular weights of CD9, CD63 and TSG101 in the DZNep treatment group were 25×103, 26×103 and 44×103, respectively. B: NTA results showed that the detection concentration of the control group was 2.7×107 particles/mL, the peak particle size was 180.5 nm, and the measured average particle size was 185.8 nm; The detection concentration of DZNep intervention group was 3.3×107 Particles/mL, the peak particle size was 161.4 nm, and the measured average particle size was 162.6 nm. C: Compared with the control group, the number and volume of exosomes in DZNep group were reduced."
Fig. 2
Analysis and comparison of EZH2 expression in various tumors A: TCGA database detection analysis showed that EZH2 expression was higher in breast cancer than in adjacent non-cancerous tissues; B: Wayne diagram showed that high EZH2 expression was positively correlated with upregulation of both immune cells and stromal cells; C: The relationship with EMT-related molecule expression showed that high EZH2 expression was positively correlated with E-cadherin, N-cadherin, and vimentin expression in BRCA, positively correlated with BRCA-Luminal B E-cadherin, negatively correlated with N-cadherin, and positively correlated with BRCA-Luminal A N-cadherin and vimentin expression."
Fig. 3
Expression of EZH2 and EMT-related proteins A: EZH2 expression was down-regulated in the DZNep treatment group compared with the control group; B: DZNep treatment group, EMT-related protein E-cadherin expression was increased and vimentin expression was decreased.*: P<0.05, compared with NC group."
Fig. 4
Expression of intercellular junction proteins in response to DZNep A: Compared with the control group, the expression of collagen Ⅵ, a microenvironment-related protein, was significantly increased in the DZNep treatment group; B: Microenvironment-associated protein claudin 5 expression was markedly increased; C: The expression of ZO-1 and aquaporin 4 was significantly decreased; D: The expression of connexin 43 and laminin was significantly decreased; E: The expression of occludin was significantly lower. *: P<0.05, compared with NC group."
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