Fig. 1

The correlation between the expression level of ITGB3 and the clinical features of lung cancer A-B: Relative mRNA expression of ITGB3 in different lung cancer tissues and lung adenocarcinoma stages was analyzed by TCGA database; C-E: Plasma exosomes of lung adenocarcinoma patients were identified by electron microscopy, NTA and Western blot, and the expression level of ITGB3 in exosomes was detected by Western blot. F: Determination of plasma exosome ITGB3 by ELISA. LUAD: Lung adenocarcinoma. LUSC: Lung squamous cell carcinoma. ****: P<0.000 1, compared with each other; ***: P<0.001, compared with each other."

Fig. 2

Construction of A549 ITGB3 overexpression stable cell line A: Western blot was performed to detect ITGB3 expression in different lung cancer cell lines; B-C: ITGB3 overexpression level of A549 cells was detected by flow cytometry and Western blot; D-F: Exosomes secreted by lung adenocarcinoma cells were identified by electron microscopy, NTA and Western blot, and the expression level of ITGB3 in exosomes was detected by Western blot."

Fig. 3

The effect of ITGB3 overexpression on A549 cell cycle and clone formation A: Flow cytometry was used to detect cell cycle in different experimental groups; B: Cell clone formation in different experimental groups. ns: No statistical difference, compared with the control group."

Fig. 4

The effect of ITGB3 overexpression on the invasion and migration ability of A549 cells A-B: Transwell chamber experiment was used to detect cell invasion and migration ability; C: Western blot experiment was used to detect cell expression of MMP9, MMP2 and GAPDH protein. ***: P<0.001, compared with control group."

Fig. 5

TRAP staining shows the effect of exosomes secreted by lung adenocarcinoma cells on the differentiation of osteoclasts. *: P<0.05, compared with control group."