Fig. 1

Expression of PRMT7 in bladder cancer cell lines Expression of total PRMT7 protein in bladder cancer cell lines 5637, T24, RT112, UM-UC-3 and ureteral epithelial immortalized cells SV-HUC-1. Compared with SV-HUC-1, total protein of PRMT7 was significantly higher in 5637 cells and significantly lower in T24 cells. *: P<0.05, compared with SV-HUC-1; **: P<0.01, compared with SV-HUC-1."

Fig. 2

Transfection efficiency of PRMT7 was verified Silenced and overexpressed PRMT7 protein and mRNA levels in bladder cancer 5637 and T24 cells. The results showed that the histone and mRNA expression levels of siPRMT7#1 and siPRMT7#2 were significantly decreased compared with si-NC, and the histone and mRNA expression levels of p-PRMT7 were significantly increased compared with p-PCMV3 group. **: P<0.01, compared with si-NC; ***: P<0.001, compared with si-NC or p-PCMV3."

Fig. 3

PRMT7 inhibits the proliferation and migration of bladder cancer cells PRMT7 gene was silenced in 5637 cells, and PRMT7 gene was overexpressed in T24 cells. A: CCK-8 assay showed that compared with the control group, down-regulation of PRMT7 promoted cell growth, while overexpression of PRMT7 was opposite. B: Colony formation experiment showed that compared with the control group, down-regulation of PRMT7 promoted cell proliferation, while overexpression of PRMT7 was opposite. C: Transwell experiment showed that compared with the control group, down-regulation of PRMT7 promoted cell migration, while overexpression of PRMT7 was opposite. D: Western blot results showed that compared with the control group, the expression of Cyclin D1, CDK4 and MMP9 increased after down-regulation of PRMT7, while the overexpression of PRMT7 was on the contrary. *: P<0.05, compared with p-PCMV3; **: P<0.01, compared with si-NC or p-PCMV3; ***: P<0.001, compared with si-NC."

Fig. 4

Notch signaling pathway related proteins were detected PRMT7 gene was silenced in 5637 cells, and PRMT7 gene was overexpressed in T24 cells. Compared with the control group, the expression of Notch1, HEY1 and Hes1 increased after down-regulation of PRMT7, while the overexpression of PRMT7 was on the contrary. *: P<0.05, compared with si-NC or p-PCMV3; **: P<0.01, compared with si-NC or p-PCMV3."

Fig. 5

PRMT7 inhibits the proliferation and migration of bladder cancer cells by regulating Notch signaling pathway 5637 cells were transfected with siPRMT7#2 and treated with DAPT. A: CCK-8 experiment showed that DAPT treatment inhibited cell growth. B: Colony formation experiment showed that DAPT treatment inhibited cell proliferation. C: Transwell experiment showed that DAPT treatment inhibited cell migration. D: Western blot results showed that compared with si-NC group, DAPT reversed the expression of Notch1, HEY1, Hes1 and the downstream proliferation and migration related target proteins Cyclin D1, CDK4, MMP9. *: P<0.05, compared with each other; **: P<0.01, compared with each other; ***: P<0.001, compared with each other."