China Oncology ›› 2023, Vol. 33 ›› Issue (7): 664-672.doi: 10.19401/j.cnki.1007-3639.2023.07.004
• Article • Previous Articles Next Articles
ZHOU Cong1(), HE Lina1, CHENG Xiaojiao1, HUANG Tinglei2, TU Shuiping1()
Received:
2022-10-30
Revised:
2023-04-25
Online:
2023-07-30
Published:
2023-08-10
Contact:
TU Shuiping.
CLC Number:
ZHOU Cong, HE Lina, CHENG Xiaojiao, HUANG Tinglei, TU Shuiping. Effect of RSPO3 on inhibiting the growth of colorectal cancer transplanted tumors and increasing NK cell infiltration in vivo[J]. China Oncology, 2023, 33(7): 664-672.
Fig. 1
Expression of RSPO3 in pancarcinoma A, B: The Oncomine database suggests that RSPO3 is relatively low expressed in a variety of human solid tumors, including colorectal cancer; C: Expression of RSPO3 in various tumor cell lines in CCLE database; D: TISIDB database showed the correlation between RSPO3 expression and various immunoinfiltrated lymphocytes, and RSPO3 expression in colorectal cancer was significantly positively correlated with NK cells. TIL: Tumor infiltrating lymphocytes. *: P<0.05; **: P<0.01; ***: P<0.001."
Fig. 2
Construction of RSPO3 knockdown or overexpressed colon cancer cell lines A: RSPO3 expression in human colon epithelial cells NCM460 and a variety of colon cancer cell lines; B: shRNA knockdown RSPO3 expression in SW480 cell line; C: Lentivirus infection induced RSPO3 overexpression in HCT116 cell line. KD: Knock down; OE: Overexpression."
Fig. 3
Effects of RSPO3 on the growth of SW480 or HCT116 cell line in vitro and in vivo A, B: The effects of RSPO3 knockdown or overexpression on cell proliferation of SW480 or HCT116 cell line were analyzed by CCK-8; C, D: Flow cytometry was used to detect the effects of RSPO3 knockdown or overexpression on SW480 or HCT116 cell cycles; E, F, G: Effects of RSPO3 knockdown on the growth of SW480 subcutaneous xenograft tumors; H, I, J: Effects of RSPO3 overexpression on the growth of HCT116 subcutaneous xenograft tumors.*: P>0.05."
Fig. 4
RSPO3 affects the proportion of NK cells in vivo A, B: Flow cytometry was used to analyze the proportion of NK cells in single cell suspension of spleen after transplantation of SW480-RSPO3-KD or SW480-Ctrl subcutaneous xenograft tumor; C, D: Flow cytometry was used to analyze the proportion of NK cells in the subcutaneous tumor of SW480-RSPO3-KD or SW480-Ctrl; E, F: Flow cytometry was used to analyze the proportion of NK cells in spleen single-cell suspension after transplantation of HCT116-RSPO3-OE or HCT116-Ctrl subcutaneous xenograft tumor; G, H: Flow cytometry was used to analyze the proportion of NK cells in the subcutaneous xenograft tumor of HCT116-RSPO3-OE or HCT116-Ctrl."
Fig. 5
Correlation analysis of RSPO3 expression level with NK cell markers and NK activated markers A: Spearman correlation analysis based on TCGA colorectal cancer database showed that RSPO3 was highly positively correlated with CD56 (NCAM1), r= 0.58, P<0.05; B: RSPO3 was highly positively correlated with CD16 (FCGR3A), r= 0.64, P<0.05; C: RSPO3 was highly positively correlated with CD69, r= 0.51, P<0.05; D: RSPO3 was moderately positively correlated with KLRB1 (r= 0.37, P<0.05)."
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