The effect of down-regulated gene PTTG1 on proliferation, apoptosis, migration and invasion of human glioma cell SHG44

doi:10.19401/j.cnki.1007-3639.2019.05.003

China Oncology ›› 2019, Vol. 29 ›› Issue (5): 338-344.doi: 10.19401/j.cnki.1007-3639.2019.05.003

The effect of down-regulated gene PTTG1 on proliferation, apoptosis, migration and invasion of human glioma cell SHG44

CUI Lishan¹, LIN Ting², XU Lanxi², WANG Guanling², LIN Jianbin¹, FENG Sanping¹, CAO Yang¹, CAO Ying¹, SONG Zhengmao¹, JIN Xin²

1. Department of Neurosurgery, Xiamen Fifth Hospital, Xiamen 361101, Fujian Province, China; 2. Department of Basic Medicine, School of Medicine, Xiamen University, Xiamen 361102, Fujian Province, China

Online:2019-05-30 Published:2019-06-03
Contact: JIN Xin E-mail: xinjin@xmu.edu.cn

Abstract

Abstract: Background and purpose: Some studies have showed that pituitary tumor-transforming gene 1 (PTTG1) is highly expressed in many cancers. This study aimed to investigate the effects of PTTG1 on the proliferation, apoptosis, migration and invasion of glioma cells SHG44. Methods: PTTG1 siRNA was used to interfere with the gene expression of SHG44 in glioma cells. Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) and Western blot were used to evaluate the PTTG1 silencing efficiency at mRNA and protein levels. Furthermore, we tested the effects of PTTG1 on proliferation, apoptosis, migration and invasion of SHG44 cells. Results: The results showed that silencing PTTG1 gene expression significantly inhibited the proliferation (P<0.05), migration (P<0.01) and invasion (P<0.001) ability of SHG44 cells, and increased its apoptosis (P<0.05). Conclusion: Decreasing the expression of PTTG1 can inhibit the degree of glioma deterioration, and it is expected to become a new target for the treatment of glioma.

Key words: Pituitary tumor-transforming gene 1, SHG44, Proliferation, Apoptosis, Migration, Invasion

CUI Lishan, LIN Ting, XU Lanxi, WANG Guanling, LIN Jianbin, FENG Sanping, CAO Yang, CAO Ying, SONG Zhengmao, JIN Xin. The effect of down-regulated gene PTTG1 on proliferation, apoptosis, migration and invasion of human glioma cell SHG44[J]. China Oncology, 2019, 29(5): 338-344.

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The effect of down-regulated gene PTTG1 on proliferation, apoptosis, migration and invasion of human glioma cell SHG44

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