China Oncology ›› 2019, Vol. 29 ›› Issue (3): 171-177.doi: 10.19401/j.cnki.1007-3639.2019.03.003

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Expression of EphA7 in osteosarcoma and its effect on proliferation, migration and apoptosis of osteosarcoma MG-63 cells

LIU Zheng1,2, WANG Jing3, SHEN Wei1,2, ZHAO Shuxuan1,2, YUAN Wenhua1,2, ZHOU Haiyu1   

  1. 1. Department of Orthopedics, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China; 2. Key Laboratory of Bone and Joint Diseases of Gansu Province, Lanzhou 730030, Gansu Province, China; 3. Department of Special Medicine, The 943th Hospital of the Chinese People’s Liberation Army, Wuwei 733000, Gansu Province, China
  • Online:2019-03-30 Published:2019-04-26
  • Contact: ZHOU Haiyu E-mail: gslzzhy2004@163.com

Abstract: Background and purpose: EphA7 protein is a membrane protein that plays an important role in the normal physiological processes of the human body, and often plays various regulatory roles in the cell membrane. However, the role of this protein in osteosarcoma remains unclear. Methods: Osteosarcoma and adjacent normal tissues of 45 patients were collected. Human osteosarcoma MG-63 cells were cultured and transfected with siRNA, and then divided into siRNA-EphA7 group (EphA7-siRNA transfection), siRNA-Control group (negative control siRNA transfection) and blank group (untransfected). Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) and Western blot were used to assess the mRNA and protein expression of EphA7 in tissues and cells. Cell proliferation viability was determined by cell counting kit-8 (CCK-8). Cell migration ability was measured by cell scratch assay. Apoptosis was detected by flow cytometry. Results: RTFQ-PCR and Western blot showed that the mRNA expression and protein levels of EphA7 were significantly higher in osteosarcoma than in adjacent normal tissues (P<0.05). The mRNA expression and protein levels of EphA7 in the siRNA-EphA7 group were significantly lower than those in the blank group and the siRNA-Control group (P<0.05). CCK-8 assay showed that the D value of the siRNA-EphA7 group was significantly lower than those of the blank group and the siRNA-Control group (P<0.05). The cell scratch assay showed that the healing rate of the siRNA-EphA7 group was significantly reduced after scratching compared with the blank group and the siRNA-Control group (P<0.05). Flow cytometry showed a significant increase in apoptotic rate in the siRNA-EphA7 group compared with the blank group and the siRNA-Control group (P<0.05). Conclusion: EphA7 is up-regulated in osteosarcoma, and down-regulation of EphA7 expression in MG-63 cells can inhibit the proliferation and migration of osteosarcoma cells and promote their apoptosis.

Key words: Osteosarcoma, EphA7, siRNA, Proliferation, Migration, Apoptosis