China Oncology ›› 2022, Vol. 32 ›› Issue (11): 1105-1114.doi: 10.19401/j.cnki.1007-3639.2022.11.009
• Review • Previous Articles Next Articles
WANG Ruotong(), WANG Xin, SHEN Bo()
Received:
2022-03-15
Revised:
2022-05-07
Online:
2022-11-30
Published:
2022-12-14
Contact:
SHEN Bo
CLC Number:
WANG Ruotong, WANG Xin, SHEN Bo. Application and progress of organoids in tumor translational medicine[J]. China Oncology, 2022, 32(11): 1105-1114.
Tab. 1
Comparison of commonly used models for malignant tumors"
Item | Cancer cell line | PDX | Tumor spheroid | Organoid |
---|---|---|---|---|
Tumor microenvironment | No | Limited | No | No |
Physiologic representation | Limited | Semiphysiologic | Limited | Semiphysiologic |
Difficulty | Easy | Hard | Easy | Easy |
Repopulation activity | Good | Medium | Poor | Good |
Genome editing | Yes | No | Yes | Yes |
High-throughput screening | Yes | No | Yes | Yes |
Tumor heterogeneity | No | Yes | No | Yes |
Library establishment | Yes | Limited | Yes | Yes |
Tab. 2
Common organoid medium components summary"
Tumor | Author and reference | Purposes | Key resources | Buffers/media | Nultrition | Antibiotic | Supplements | Others |
---|---|---|---|---|---|---|---|---|
Lung cancer | Shi R,et al[ | Drug response including targeting drugs; Establishing a platform | Advanced DMEM/F12, glutamax, HEPES, antibiotic-antimycotic, B-27 supplement, N-acetylcysteine, rec. hEGF, rec. hFGF-10, rec. hFGF-4, rec. hNoggin, SAG (smoothened ligand), A83-01 (TGF-b receptor inhibitor), Y-27632, CHIR 99021(GSK-3 inhibitor) | Advanced DMEM/F12, HEPES | GlutaMAX, B-27 supplement, N-acetylcysteine | Antibiotic-antimycotic | Rec. hEGF, rec. hFGF-10, rec. hFGF-4, rec. hNoggin, SAG (smoothened ligand) | A83-01 (TGF-b receptor inhibitor), Y-27632, CHIR 99021 (GSK-3 inhibitor) |
Liver cancer | Broutier L, et al[ | Prognosis indicators; drug susceptibility prediction; Identification of new targets | Advanced DMEM/F12, 1% penicillin/streptomycin, 1% glutamax, HEPES, B27 supplement (without vitamin A), N2 supplement, N-acetylcysteine, R-spondin1 (conditioned medium), nicotinamide, rec. h[Leu15]-gastrin Ⅰ, rec. hEGF, rec. hFGF10, rec. hHGF, forskolin (CAMP inhibitor), A8301, Y27632 (remove after passaging) and dexamethasone (remove after passaging) | Advanced DMEM/F12, HEPES | 1% glutamax, B27 supplement (without vitamin A), N2 supplement, N-acetylcysteine, R-spondin1 (conditioned medium), nicotinamide | 1% Penicillin/Streptomycin | Rec. h[Leu15]-gastrin Ⅰ, rec. hEGF, rec. hFGF10, rec. hHGF | Forskolin (CAMP inhibitor), A8301, Y27632 (remove after passaging) and dexamethasone (remove after passaging) |
Rectal cancer | Ganesh K, et al[ | Establishing a platform, radiotherapy, chemotherapy and targeted drug response prediction | Advanced DMEM/F12 supplemented with antibiotic-antimycotic, B27, N2, glutamax, gastrin Ⅰ, HEPES, N-acetylcysteine, nicotinamide, 50% Wnt-3A (conditioned medium and remove after passaging), 20% R-spondin-1 (conditioned medium and remove after passaging), rec. mNoggin (remove after passaging), rec. hEGF, A83-01, SB202190 | advanced DMEM/F12, HEPES | B27, N2, glutamax, N-acetylcysteine, nicotinamide | Antibiotic-antimycotic | Gastrin Ⅰ, 50% Wnt-3A (conditioned medium and remove after passaging), 20% R-spondin-1 (conditioned medium and remove after passaging), rec. mNoggin (and remove after passaging), rec. hEGF | A83-01, SB202190 |
Gastric cancer | Bartfeld S, et al[ | Culture protocol, modeling organogenesis | Advanced DMEM/F12 supplemented with penicillin/ streptomycin, HEPES, glutamax, B27, N-acetylcysteine, EGF, Noggin (conditioned medium) 10%, R-spondin1 (conditioned medium) 10%, Wnt (conditioned medium) 50%, FGF10, gastrin, TGF-βi (A-83-01). nicotinamide*, RHOKi (Y-27632), IGF*, p38 inhibitor (SB202190)*, GSK3β inhibitor (CHIR99021)*, PGE2* | advanced DMEM/F12, HEPES | Glutamax, B27, N-Acetylcysteine | Penicillin/streptomycin | EGF, Noggin conditioned medium 10%, 10% R-spondin1 (conditioned medium), 50% Wnt (conditioned medium), FGF10, gastrin, IGF* | TGFβi (A-83-01), RHOKi (Y-27632), p38 inhibitor (SB202190)*, GSK3β inhibitor (CHIR99021)*, PGE2* |
Gastric cancer | Yan H H N, et al[ | Tumor heterogeneity; drug susceptibility prediction | Modifying the medium of Bartfeld S by adding nutlin-3a (p53/MDM2 inhibitor) | advanced DMEM/F12, HEPES | Glutamax, B28, N-Acetylcysteine | Penicillin/streptomycin | Rec. hEGF, rec. hFGF10, Wnt3A (conditioned medium), R-spondin1 (conditioned medium), Noggin (conditioned medium) | Nutlin-3a (p53/MDM2 inhibitor) |
Pancreatic cancer | Tiriac H,et al[ | Biomarker, genome character, prognosis, drug response prediction | Advanced DMEM/F12, HEPES, glutamax, A83-01, hEGF, mNoggin, hFGF10, hgastrin Ⅰ, N-acetylcysteine, nicotinamide, PGE2, B27 supplement, R-spondin1 (conditioned media), Afamin/Wnt3A (conditioned media) | Advanced DMEM/F12, HEPES | Glutamax, N-acety-lcysteine, nicotinamide, B27 supplement | NM | Rec. hEGF, Afamin/Wnt3A, rec. hFGF10, rec. hgastrin I, PGE2, R-spondin1 (conditioned media), Afamin/Wnt3A (conditioned media) | A83-01 |
Prostate cancer | Chua C W, et al[ | Modeling prostate organoid | Hepatocyte culture medium, EGF, Y-27632, glutamax, 5% activated carbon stripped FBS | Hepatocyte culture medium | 5% activated carbon stripped FBS, glutamax | NM | EGF | Y-27632 |
Bladder cancer | Lee S H,et al[ | Establish PDO biobank and describe character | Hepatocyte culture medium, EGF, Y-27632, glutamax, 5% activated carbon stripped FBS | Hepatocyte culture medium | 5% activated carbon stripped FBS, glutamax | NM | EGF | Y-27632 |
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