China Oncology ›› 2023, Vol. 33 ›› Issue (1): 45-53.doi: 10.19401/j.cnki.1007-3639.2023.01.005
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WANG Xiaoxiao1(), CHEN Xi1, LI Minmin1, SONG Ning1, SUN Dongyuan1, JIANG Yingying1,2()
Received:
2022-06-01
Revised:
2022-08-03
Online:
2023-01-30
Published:
2023-02-13
Contact:
JIANG Yingying
CLC Number:
WANG Xiaoxiao, CHEN Xi, LI Minmin, SONG Ning, SUN Dongyuan, JIANG Yingying. Effects of NOL8 on cell proliferation, migration and invasion of oral squamous cell carcinoma[J]. China Oncology, 2023, 33(1): 45-53.
Fig. 1
The relative expression of NOL8 in OSCC tissues and cell lines A: TIMER database showed the expression of NOL8 in head and neck squamous cell carcinoma and adjacent normal tissues from green box. B: Expression of NOL8 in HNSC shown in GEPIA2 database; T: Tumor; N: Nornal. C: NOL8 expression in HNSC shown from ENCORI database. D: UALCAN database showed the expression of NOL8 in HNSC. E: RTFQ-PCR assay was used to detect the expression of NOL8 in HN6, CAL-27 and normal cell. *: P<0.05; **: P<0.01; ***: P<0.001."
Fig. 2
Effect of knocking down NOL8 expression on the cell proliferation of OSCC A: The relative expression of NOL8 in the CAL-27 cells was detected by RTFQ-PCR after knocking down NOL8. B: CCK-8 assays were used to detect the proliferation ability of CAL-27 cells after NOL8 knockdown. **: P<0.01, compared with NC; ****: P<0.000 1, compared with si-NOL8-1 and si-NOL8-2."
Fig. 3
Effect of knocking down NOL8 expression on cell migration and invasion of OSCC A: Scratch healing assay showed that the migration ability of CAL-27 cells after NOL8 was inhibited. B: Transwell assay showed that the invasion ability of CAL-27 cells after NOL8 was inhibited. **: P <0.01, compared with NC; ****: P<0.0001, compared with NC; ***: P<0.001, compared with NC."
Fig. 4
Effect of overexpression of NOL8 on cell proliferation of OSCC A: The relative expression of NOL8 in the CAL-27 and HN6 cells was detected by RTFQ-PCR after over-expressing NOL8. B: CCK-8 assays were used to detect the proliferation ability of CAL-27 and HN6 cell after NOL8 was overexpressed. **: P<0.01, ****: P<0.000 1."
Fig. 5
Effect of overexpression of NOL8 on cell migration and invasion of OSCC A: Scratch healing assay showed that the migration ability of CAL-27 and HN6 cells after overexpression of NOL8. B: Transwell assay showed that the invasion ability of CAL-27 and HN6 cells after overexpression of NOL8. ***: P<0.001, compared with control; ****: P<0.000 1, compared with control."
Fig. 6
Effect of the change of NOL8 expression on EMT-related proteins in CAL-27 and HN6 cells Western blot assay showed the protein level of N-cadherin, E-cadherin and vimentin after inhibition of NOL8 expression in CAL-27 cells and overexpression of NOL8 in CAL-27 and HN6 cells. **: P <0.01, compared with control; ***: P<0.001, compared with NC or control; ****: P<0.000 1, compared with NC or control."
Fig. 7
Effect of NOL8 expression on the growth of subcutaneously xenograft tumor in nude mice A: OSCC xenograft tumor formed by CAL-27 cells after overexpression of NOL8. B: The weight of OSCC xenograft tumor. C: H-E and Ki-67 staining of OSCC xenograft tumor tissue of CAL-27 cells after overexpression of NOL8. *: P<0.05, compared with control."
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